Nanometer titanium dioxide-loaded L-serine imprinted polymer thin layer plate and preparation method thereof

Abstract

The invention provides a nanometer titanium dioxide-loaded L-serine imprinted polymer thin layer plate and a preparation method thereof. According to the invention, nanometer titanium dioxide is used as a carrier, L-serine is used as a template, and an L-serine molecularly imprinted polymer is prepared by using a surface imprinting method; the surface of titanium dioxide is activated by using a nitric acid solution, then gamma-MAPS is used for modifying activated titanium dioxide, so functional groups on the surface of a solid is improved, and the carrier has improved capability in surface grafting with the L-serine molecularly imprinted polymer; and then the thin layer plate is prepared from the obtained molecularly imprinted polymer, n-butanol-glacial acetic acid-water (V / V, 4: 1: 1) of ninhydrin with a concentration of 0.1% is used as a developing solvent, and when the developing solvent is used for separation of chiral compounds L-serine and D-serine, a relative Rf value is greater than 2.0, so L-serine and D-serine are separated. Thus, the problem that conventional thin-layer chromatography and conventional liquid chromatography cannot realize separation of a stationary phase is overcome, and separation of a molecularly imprinted thin layer of chiral amino acid compounds is realized. The invention provides a novel material and method for chiral resolution and compartment analysis of serine.

Description

technical field

[0001] The invention relates to a thin-layer board loaded with L-serine imprinted polymer on the surface of nano-titanium dioxide and a preparation method thereof. Background technique

[0002] Molecularly imprinted polymers (MIPs) are polymers synthesized by molecular imprinting technology that can specifically recognize and selectively adsorb specific target molecules (template molecules) and their structural analogs. MIP preparation principle: mix imprinted molecules (template molecules) with suitable functional monomers, and polymerize them with a cross-linking agent to form a three-dimensional cross-linked polymer network with a high degree of cross-linking around the imprinted molecules, and then The imprinted molecules are then removed by an appropriate method, and the final polymer obtained is a molecularly imprinted polymer. MIP has the properties of recognition, predetermined structure and activity, and practicability, so it has broad application p...

Examples

Embodiment Construction

[0014] The nano-titanium dioxide loaded L-serine imprinted polymer thin-layer plate and its preparation method are described in detail below.

[0015] (1) Pretreatment of nano-titanium dioxide: accurately weigh 6.0g nano-TiO 2 Bake at 353K for 12 hours, cool down, and measure the volume V 1 =20mL concentrated nitric acid solution, moved to a 100mL volumetric flask to prepare a nitric acid solution with a concentration of 3.2mol / L, and then the nano-TiO 2 After soaking in 3.2mol / L nitric acid solution for 12 hours, vacuum filter and wash the precipitate with deionized water to pH 7.0, and dry it in vacuum for 6 hours at 333K, and set aside;

[0016] (2) Surface modification of nano-titanium dioxide: weigh 2.0g of activated nano-TiO 2 , add 4 mL of γ-MAPS-methanol mixed solution [V / V, 1:1], react at 313K for 12 hours, cool, grind, wash with 50 mL of methanol and water to remove solids, dry, and set aside;

[0017] (3) Preparation of nano-titanium dioxide surface-grafted impri...

technical field

[0001] The invention relates to a thin-layer board loaded with L-serine imprinted polymer on the surface of nano-titanium dioxide and a preparation method thereof. Background technique

[0002] Molecularly imprinted polymers (MIPs) are polymers synthesized by molecular imprinting technology that can specifically recognize and selectively adsorb specific target molecules (template molecules) and their structural analogs. MIP preparation principle: mix imprinted molecules (template molecules) with suitable functional monomers, and polymerize them with a cross-linking agent to form a three-dimensional cross-linked polymer network with a high degree of cross-linking around the imprinted molecules, and then The imprinted molecules are then removed by an appropriate method, and the final polymer obtained is a molecularly imprinted polymer. MIP has the properties of recognition, predetermined structure and activity, and practicability, so it has broad application p...

Examples

Embodiment Construction

[0014] The nano-titanium dioxide loaded L-serine imprinted polymer thin-layer plate and its preparation method are described in detail below.

[0015] (1) Pretreatment of nano-titanium dioxide: accurately weigh 6.0g nano-TiO 2 Bake at 353K for 12 hours, cool down, and measure the volume V 1 =20mL concentrated nitric acid solution, moved to a 100mL volumetric flask to prepare a nitric acid solution with a concentration of 3.2mol / L, and then the nano-TiO 2 After soaking in 3.2mol / L nitric acid solution for 12 hours, vacuum filter and wash the precipitate with deionized water to pH 7.0, and dry it in vacuum for 6 hours at 333K, and set aside;

[0016] (2) Surface modification of nano-titanium dioxide: weigh 2.0g of activated nano-TiO 2 , add 4 mL of γ-MAPS-methanol mixed solution [V / V, 1:1], react at 313K for 12 hours, cool, grind, wash with 50 mL of methanol and water to remove solids, dry, and set aside;

[0017] (3) Preparation of nano-titanium dioxide surface-grafted impri...