Preparation of a cyclodextrin-cuag electrochemical immunosensor

An immune sensor and cyclodextrin technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of poor application, low sensitivity, high cost, etc., achieve polymer recognition ability, and short response time , the effect of low detection limit

Active Publication Date: 2015-08-19
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the methods for detecting tumor markers mainly include radioimmunoassay, enzyme-linked immunoassay, chemiluminescence immunoassay, and electrochemical immunoassay. get well applied

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of a Cyclodextrin-CuAg Electrochemical Immunosensor

[0038] A glassy carbon electrode with a diameter of 4 mm was covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0039] Take 6 μL, 0.5 mg·mL -1 Cyclodextrin-functionalized graphene solution is added dropwise to the electrode surface, dried at room temperature, rinsed with ultrapure water, and dried;

[0040] Add dropwise 6 μL, 5 μg·mL -1 The antibody functionalized with adamantanecarboxylic acid, after drying, rinse the surface of the electrode with ultrapure water, and dry it in a refrigerator at 4°C;

[0041] Add 3 μL of BSA solution with a mass fraction of 1% dropwise to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;

[0042] Add 6 μL, 0.0001-20 ng / mL of a series of antigen solutions with different concentrations, and after drying, rinse the surface ...

Embodiment 2

[0044] Example 2 Preparation of a Cyclodextrin-CuAg Electrochemical Immunosensor

[0045] A glassy carbon electrode with a diameter of 4 mm was covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0046] Take 6 μL, 1.0 mg·mL -1 Cyclodextrin-functionalized graphene solution is added dropwise to the electrode surface, dried at room temperature, rinsed with ultrapure water, and dried;

[0047] Add dropwise 6 μL, 7.5 μg·mL -1 The antibody functionalized with adamantanecarboxylic acid, after drying, rinse the surface of the electrode with ultrapure water, and dry it in a refrigerator at 4°C;

[0048] Add 3 μL of BSA solution with a mass fraction of 1% dropwise to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;

[0049] Add 6 μL, 0.0001-20 ng / mL of a series of antigen solutions with different concentrations, and after drying, rinse the surfac...

Embodiment 3

[0051] Example 3 Preparation of a Cyclodextrin-CuAg Electrochemical Immunosensor

[0052] A glassy carbon electrode with a diameter of 4 mm was covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0053] Take 6 μL, 1.5 mg·mL -1 Cyclodextrin-functionalized graphene solution is added dropwise to the electrode surface, dried at room temperature, rinsed with ultrapure water, and dried;

[0054] Add 6 μL, 10 μg·mL dropwise -1 The antibody functionalized with adamantanecarboxylic acid, after drying, rinse the surface of the electrode with ultrapure water, and dry it in a refrigerator at 4°C;

[0055] Add 3 μL of BSA solution with a mass fraction of 1% dropwise to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;

[0056] Add 6 μL, 0.0001-20 ng / mL of a series of antigen solutions with different concentrations, and after drying, rinse the surface...

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Abstract

The invention discloses a method for preparing cyclodextrin-based functionalized graphene, cyclodextrin-Cu@Ag and adamantane formic acid functionalized antibodies and a constructed electrochemical immunosensor for qualitative and quantitative detection of tumor markers, and relates to multiple fields of biotechnology, material chemistry, nano science and the like. By adopting the cyclodextrin-based functionalized nano material, the affinity to biomolecules of antigens, antibodies and the like is enhanced, and the fixed quantity and the fixed effect of the biomolecules are improved; and by using adamantane formic acid functionalized antibody molecules, multifunctional marking of the antibodies is realized, and the detection sensitivity of the sensor is improved.

Description

Technical field [0001] The present invention involves the preparation and application of an cyclone-CUAG electrochemical immune sensor.Specifically, the cycloped-CUAG nuclear shell nanomaterial is used to prepare a variety of sandwiched electrochemical immune sensors that detect tumor logo, which belongs to the field of new functional materials and biosensor detection technology. Background technique [0002] Malignant tumors are commonly known as cancer. When the cells have mutations in the body, it will constantly split, not controlled by the body, and eventually form cancer.The cells of malignant tumors can violate and destroy neighboring tissues and organs.Moreover, cancer cells can be worn from the tumor and enter the blood or lymphatic system, causing cancer metastasis. [0003] Tumor logo is a chemical substance that reflects the existence of tumors. Their existence or quantitative changes can indicate the nature of the tumor to understand the occurrence of tumor tissue, c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N33/574
Inventor 魏琴高健杜斌吴丹张勇马洪敏胡丽华
Owner UNIV OF JINAN
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