Isaria farinose HS05 and application thereof

A powder stick and strain technology, applied in the field of biotechnology prevention and control, can solve problems such as affecting human health, single species of pesticides, accumulation of pesticide residues, etc., and achieve the effect of strong broad-spectrum pathogenicity

Pending Publication Date: 2016-10-12
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, most of the pesticides used to prevent and control forestry pests are chemical insecticides, and there are only a single variety of pesticides, and many pests have developed serious resistance to many varieties.
In addition, due to the long-term use

Method used

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  • Isaria farinose HS05 and application thereof
  • Isaria farinose HS05 and application thereof
  • Isaria farinose HS05 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The separation and identification of embodiment 1 I. clavula HS05

[0022] I. clavum HS05 was isolated from the larvae of the dead oak in Huangshan Scenic Area. Methods as below:

[0023] Pick the spores of the larvae of the larvae of the larvae of Alpinia spp. with mycelia collected in Huangshan, transfer them to the SDAY culture with antibiotics, and culture them at 25°C for 5 days. After colonies are formed on the culture medium, pick the hyphae in the center of the colonies and transfer them to SDAY medium newly added with antibiotics for purification. A bacterial strain obtained by screening was numbered HS05.

[0024] 1) Morphological identification: Inoculate the purified strains on SDAY medium, put them in an incubator at 25°C for 5 days, take them out, observe the front and back of the colonies, and stick a few of them in the culture dish with transparent tape. For the mycelia, distilled water was used as a floating carrier, and a temporary glass slide was p...

Embodiment 2

[0029] Use SDAY medium as the basic medium, make plates after autoclaving, and transfer to the center of each plate The purpose of biocontrol bacteria cake. Cultured at 6 different temperature gradients of 5°C, 10°C, 15°C, 20°C, 25°C, and 30°C, respectively, with 4 plates for each replicate, 3 replicates in total, taken out after 5d and 10d, and used the cross method The colony diameter was measured, and the calculation results are shown in Table 1.

[0030] Table 1 HS05 bacterial strain different temperature environment bacterial colony growth velocity measurement result

[0031] Processing temperature (℃)

[0032] The results show that I. clavoniae HS05 can grow at a temperature of 5°C to 30°C, and the optimum growth temperature is 25°C. It can be seen from the data that the bacteria can still grow bacteria at a low temperature of 5°C to 10°C. Silk, combined with the forest climate, the fungus can colonize the forest.

Embodiment 3

[0034] Use SDAY medium as the basic medium, make plates after autoclaving, and transfer to the center of each plate The purpose of biocontrol bacteria cake. Cultured at 6 different temperature gradients of 5°C, 10°C, 15°C, 20°C, 25°C, and 30°C, respectively, with 4 plates for each replicate, 3 replicates in total, took out after 7 days, and scraped the growth on the plate completely Put the mycelium into a conical flask, add 50mL of sterile water to break up in a magnetic stirrer, filter out the mycelium, and make a fresh spore suspension. The number of spores was measured with a hemocytometer, and the calculation results are shown in Table 2.

[0035] Table 2HS05 bacterial strain different temperature environment bacterial colony growth velocity measurement result

[0036] Processing temperature (℃)

[0037] The results showed that the spore yield of I. clavoniae HS05 varied greatly at different temperatures after 7 days, and the optimum culture temperature was a...

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Abstract

The invention discloses isaria farinose HS05 and application. The classification of isaria farinose is named as Isaria farinose HS05, the strain is preserved in China General Microbiological Culture Collection Center on May, 9, 2016, the preservation number is CGMCC No.12247, and the preservation address is No.3, Courtyard No.1, Beichen West Road, Chaoyang District, Beijing. The isaria farinose HS05 is high in sporulation capacity and high in insecticidal activity on larvae of aphrodisium sauteri matsushita, and the fatality rate is larger than 80%. By means of the isaria farinose HS05, the population quantity of the aphrodisium sauteri matsushita can be effectively controlled. In addition, the isaria farinose has the excellent control effect on other forest pests such as dendrolimus spp., monochamus alternatus hope and hyphantria cunea, and very good application and popularization value is achieved.

Description

technical field [0001] The invention belongs to the technical field of biotechnology prevention and control, and in particular relates to the preparation of Isaria farinosa HS05 and its bacterial agent and its application in preventing and controlling forestry insect pests. Background technique [0002] Forestry pests are the most important factor affecting forestry output in modern forestry production. According to the State Forestry Administration, there are more than 8,000 species of forestry pests in my country, and more than 200 species can cause serious damage. Since 2007, my country's forestry pests have occurred in an area of ​​more than 175 million mu every year, accounting for 50.69% of the total area of ​​forestry disasters, dozens of times the area of ​​forest fires, and causing an average annual loss of 110.1 billion yuan. With the adjustment of forestry industrial structure, the control and prevention of insect pests are paid more and more attention by people. ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 周立峰陈凤毛吴小扁王文秀
Owner NANJING FORESTRY UNIV
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