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Novel method for gastric cancer diagnosis

A technology for gastric cancer and analysis methods, which can be applied in the fields of material inspection products, instruments, analysis materials, etc., and can solve the problem that the sugar chain structure does not reveal the accurate scientific basis and so on.

Inactive Publication Date: 2016-10-12
KOREA ADVANCED INST OF SCI & TECH
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Problems solved by technology

It is well known that glycosylation, one of the processes of post-translational modification, can mark the development of tumors, but so far the exact scientific basis for the structure of sugar chains in tumors has not been revealed

Method used

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  • Novel method for gastric cancer diagnosis
  • Novel method for gastric cancer diagnosis
  • Novel method for gastric cancer diagnosis

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Embodiment Construction

[0101] The present inventors observed that specific fucosylation and high antennary structures in haptoglobin were significantly increased in not only patients with early gastric cancer but also patients with advanced gastric cancer as compared with the normal control group. Abnormal glycosylation of haptoglobin in gastric cancer patients After immunoaffinity purification of serum-derived haptoglobin, according to lectin blotting, MALDI-TOF MS, chip-based nano-LC / TOF-MS (Chip / TOF) and The combination of multiple glycomic analyzes such as targeted MS / MS of fragmentation by collision-induced dissociation (CID) was demonstrated.

[0102] The results of the present invention clearly show that compared with the normal control group, the AAL and PHA-L lectin imprinting in the serum haptoglobin of patients with gastric cancer are higher ( figure 1 ). In order to identify proteins with abnormal sugar chains in serum, the present inventors performed a method that combined SDS-PAGE, mu...

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Abstract

The present invention relates to a method for gastric cancer diagnosis through the detection of glycan changes, and to a kit for gastric cancer diagnosis. More specifically, based on, among the changes in N-linked glycosylation of the gastric cancer patient-derived haptoglobin, which are detected through lectin and mass spectrometry, the increase in fucosylation, the increases or significant changes in particular glycan structures according to the classification of antennary, or the reduction in high-mannose structure of the N-linked glycan compared with normal persons, the discovered N-glycosylation structures can be favorably used for a method for gastric cancer diagnosis using lectin, as a diagnostic marker, or mass spectrometry, and a kit for diagnosing gastric cancer.

Description

technical field [0001] The present invention relates to a novel method for diagnosing gastric cancer by detecting changes in sugar chains, a kit for diagnosing gastric cancer, and a method for detecting changes in sugar chains for providing diagnostic information for gastric cancer, and more particularly to a blood source using lectins and detected by a mass analysis method A method for diagnosing gastric cancer with changes in the N-sugar chain of haptoglobin and a kit for diagnosing gastric cancer. Background technique [0002] Cancer is the number one cause of death worldwide, and it is also true in Korea. Cancer is formed and developed by genetic and environmental factors, and the number of deaths from cancer and cancer is increasing every year due to changes in dietary life, serious environmental pollution, and severe exposure to environmental and mental stress. Compared with other diseases, cancer is characterized by intractable cure and average low survival rate afte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/574G01N33/68
Inventor 金政会李星铉朴胜烈金镇万安贤珠金载韩吴明珍
Owner KOREA ADVANCED INST OF SCI & TECH
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