33 results about "N linked glycans" patented technology

Provided are compositions comprising one or more isoforms of an erythropoietin (“EPO”) comprising glycans linked thereto, wherein the glycans have Lewis x structures and on average at least six sialic acid moieties per EPO molecule. Further provided are methods for obtaining a composition comprising one or more isoforms of EPO comprising glycans linked thereto, wherein the glycans comprise on average at least six sialic acids per EPO molecule and from zero to two Lewis x structures, the method comprising: a) providing a eukaryotic cell containing a nucleic acid sequence encoding an adenoviral E1A protein in expressible format and a nucleic acid encoding EPO in expressible format, wherein the cell further contains a nucleic acid sequence encoding a sialyltransferase, e.g., an α-2,6-sialyltransferase or an α-2,3-sialyltransferase, under control of a heterologous promoter; b) culturing the cell in a serum-free culture medium and allowing expression of EPO in the cell; c) harvesting the expressed EPO from the cell and / or from the culture medium; and d) purifying and fractionating the EPO to obtain fractions that have an increased average sialic acid content of the N-linked glycans per EPO molecule, to obtain a composition comprising one or more isoforms of an EPO comprising glycans linked thereto, wherein the glycans comprise on average at least six sialic acids per EPO molecule and from zero to two Lewis x structures.

Disclosed herein are glycan-modified anti-CD4 monoclonal antibodies with N-linked glycans attached to the variable region. Expression vectors and cell lines useful for the production of such antibodies, and use of such antibodies for HIV prevention and therapy are also disclosed.

The present invention provides recombinant cells that contain a modification causing altered expression or function of at least one mannosyl transferase enzyme. As a result of the modification the cells produce a glycoprotein or glycopeptide that has an N-linked glycan profile that is simplified or humanized. The glycoprotein or glycopeptide can have at least 25% fewer high mannose structures on than the glycoprotein or glycopeptide produced by a cell that does not have the modification. In some embodiments the modification is a deletion, knock out, or disruption of a gene encoding a mannosyl transferase, which can be in an Alg3 gene. Therefore, the proteins produced avoid many of the problems associated with the therapeutic use of glycoproteins from species having foreign or plant-like patterns of glycosylation. The invention also provides compositions of the glycoproteins or glycopeptides and methods of making them.

A fixed-length alpha-numeric code for representing N-linked glycan structures commonly found in secreted glycoproteins from mammalian cell cultures. The code employs a pre-assigned alpha-numeric index to represent the monosaccharides attached in different branches to the core glycan structure. The present branch-centric representation allows visualization of the structure while the numerical nature of the code makes it machine readable. A difference operator can be defined to quantitatively differentiate between glycan structures for further analysis. The code can be incorporated in a retrievable format into an information management system. A method is also provided for representing the structure of at least a portion of an oligosaccharide, using the fixed-length alpha-numeric code.

Disclosed herein are glycan-modified anti-CD4 monoclonal antibodies with N-linked glycans attached to the variable region. Expression vectors and cell lines useful for the production of such antibodies, and use of such antibodies for HIV prevention and therapy are also disclosed.

A method for detecting a site which can be modified with an N-linked glycan chain and to which an N-linked glycan chain is actually linked in a glycoprotein; and a method for determining the state of an N-linked glycan chain addition at the site are provided. A glycoprotein having an N-linked glycan chain linked thereto is subjected to an N-linked glycan chain removal treatment with a peptide N-glycanase, subsequently a site capable of being modified with an N-linked glycan chain, in which an Asn residue has been changed to an Asp residue by the action of the peptide N-glycanase, is treated with an endo-type peptidase capable of recognizing an Asp residue to thereby produce peptide fragments, and subsequently the mass of the fragments is detected. In this manner, a site which can be modified with an N-linked glycan chain and to which an N-linked glycan chain is actually linked can be detected. Furthermore, the proportion or state of of the N-linked glycan chain addition at the site can be determined from the intensity of a signal generated upon the detection.

The invention discloses a characteristic N-linked sugar chain of a subcellular structure and an application thereof, belonging to the technical field of glycobiology. In the present invention, the bladder YTS1 cells are used as experimental materials, and the protein components of four subcellular structures (microsomes, mitochondria, nuclei and cytoplasm) are obtained by using differential centrifugation, and the method of combining lectin chip and mass spectrometry (MS) The glycosylation modification types and structural changes of identified subcellular structural proteins showed the distribution of sugar chains in subcellular structural differences. The present invention obtains the characteristic N-linked glycans of the subcellular structure of 10 kinds of human bladder YTS1 cells; using the characteristic N-linked glycans, the indirect detection, qualitative or quantitative of other target substances in the corresponding subcellular structure can be realized detection.

The invention provides a high-efficiency separation preparation method for preparing an N-linked polysaccharide from ovalbumin. The main component of the N-linked polysaccharide is an N-linked polysaccharide component linked with only one amino acid asparagine (Asn). The method comprises the following steps: carrying out enzymatic hydrolysis on the ovalbumin by using a non-specific protease, centrifuging the obtained enzymatic hydrolysis solution, concentrating the centrifuged solution, removing polypeptides and residual proteins obtained after the ovalbumin enzymatic hydrolysis on the concentrated solution through using a one-dimensional chromatographic column, and collecting the target fraction; and centrifuging the collected fraction, concentrating the centrifuged fraction, and allowing the concentrated fraction to go through a two-dimensional chromatographic column to remove salt, 2-4 amino acids-linked N-connected polysaccharide components and micro-molecular amino acids in the fraction in order to obtain the N-linked polysaccharide component only linked with asparagines. A result of high resolution mass spectrum identification of the Asn linked N-linked polysaccharide component prepared in the invention shows that the N-linked polysaccharide component has clear composition, and the extraction and separation method has the advantages of high repeatability and good maneuverability, and is suitable for large-scale separation and preparation of the N-linked polysaccharide component from the ovalbumin.