Culture medium for skin keratinocyte and culture method thereof
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A skin keratin and culture method technology, applied in the field of cell culture, can solve the problems of fibroblast contamination, low cell proliferation rate, easy cell differentiation, etc., and achieve the effects of reducing pollution, good refraction, and improving proliferation rate
Active Publication Date: 2016-10-26
广东省华桑丽皙生物技术有限公司
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[0007] In order to overcome the deficiencies in the prior art that keratinocytes are easy to differentiate, easily polluted by fibroblasts, easily oxidized, have a long time to adhere to the wall and low cell proliferation rate in the in vitro culture process of keratinocytes, the purpose of the present invention is to provide a skin Culture medium of keratinocytes and culture method thereof, to solve the above-mentioned problems
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Embodiment 1
[0030] Embodiment 1, a kind of culture medium of skin keratinocyte
[0031] The skin keratinocyte culture medium includes high-glucose DMEM basal medium, and also includes the following components and their concentrations: epidermal growth factor 2ng / ml, transforming growth factor-beta 3ng / ml, vitamin H 1ug / ml, recombinant human insulin 6mg / ml, transferrin 5ug / ml, hesperidin 10umol / L and resveratrol 6umol / L.
[0032] Preparation:
[0033] Add epidermal growth factor, transforming growth factor, vitamin H, recombinant human insulin, transferrin, hesperidin and resveratrol to the DMEM basal medium according to the stated concentration, mix well, and pass through the membrane to eliminate bacteria.
Embodiment 2
[0034] Embodiment 2, a kind of culture medium of skin keratinocyte
[0035] The skin keratinocyte culture medium includes high-glucose DMEM basal medium, and also includes the following components and their concentrations: epidermal growth factor 3ng / ml, transforming growth factor-beta 4ng / ml, vitamin H 2ug / ml, recombinant human insulin 8mg / ml, transferrin 6ug / ml, hesperidin 16umol / L and resveratrol 10umol / L.
[0036] The preparation method is similar to Example 1.
Embodiment 3
[0037]Embodiment 3, a kind of culture medium of skin keratinocyte
[0038] The skin keratinocyte culture medium includes high-glucose DMEM basal medium, and also includes the following components and their concentrations: epidermal growth factor 4ng / ml, transforming growth factor-beta 5ng / ml, vitamin H 3ug / ml, recombinant human insulin 9mg / ml, transferrin 8ug / ml, hesperidin 20umol / L and resveratrol 12umol / L.
[0039] The preparation method is similar to Example 1.
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Abstract
The invention belongs to the field of cell culture, and in particular relates to a culture medium for skin keratinocyte and a culture method thereof. The culture medium for the skin keratinocyte provided by the invention comprises a DMEM (Dulbecco Modified Eagle Medium) basic culture medium, an epidermal growth factor, a transforming growth factor, vitamin H, recombinant human insulin, transferrin, hesperidin and resveratrol. By adopting the culture medium for the skin keratinocyte provided by the invention, the cell attachment time of the keratinocyte can be shortened, differentiation of the keratinocyte is prevented, and the pollution of keratinocyte caused by fibroblasts is lowered; meanwhile, by adopting the culture medium for the skin keratinocyte, the proliferation rate of the keratinocyte can be increased, and the vitality and cell characteristics of the keratinocyte in a passage process are maintained; the culture medium is a relatively ideal in-vitro culture medium for the skin keratinocyte.
Description
technical field [0001] The invention belongs to the field of cell culture, and in particular relates to a medium for skin keratinocytes and a culture method thereof. Background technique [0002] Human skin is composed of three parts: epidermis, dermis and subcutaneous tissue. Keratinocytes are a type of keratinocytes in the epidermis and are an important component of the epidermis. Under normal circumstances, human epidermal cells proliferate, differentiate and shed regularly, maintain the balance of skin sugar, protein, fat, water and electrolytes, and have functions such as self-protection, body temperature regulation, waste excretion and immune regulation. [0003] Keratinocytes are the main component of epidermal cells and are epithelial cells that synthesize keratin. In the process of the evolution of keratinocytes to keratinocytes, they can generally be divided into four layers, namely, basal layer, spinous layer, granular layer and stratum corneum. Some people ref...
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