Device and method for observing micro-droplets

Abstract

The invention relates to a device for observing micro-droplets. The device comprises a micro-fluidic chip, a flickering light source and an image acquisition module, wherein the micro-fluidic chip is provided with microchannels containing cyclically moving micro-droplets; the flickering light source generates flickering light with preset flickering parameters according to a control signal provided by a flickering light source control module; the image acquisition module acquires images of the micro-droplets irradiated by the flickering light and located in the microchannles of the micro-fluidic chip. The flickering light source is controlled by the flickering light source control module to generate flickering light with specific frequency and irradiates the micro-fluidic chip; then, the image acquisition module acquires the images on the micro-fluidic chip; information about sizes, shapes, flux and the like of the micro-droplets can be obtained according to the images and the frequency of the flickering light. Compared with methods using conventional high-speed cameras, phototubes and the like, the device has the advantages that comprehensive information is acquired conveniently and the device is low in cost and easy to establish and can be widely applied to a small micro-fluidic system.

Description

technical field [0001] The invention relates to a device and a method for observing micro-droplets, in particular to a device and method for observing micro-droplets in microfluidics aimed at high-throughput and periodic micro-droplets. Background technique [0002] Microfluidic chip (microfluidic chip), also known as lab on a chip, refers to the integration or basic integration of basic operating units such as sample preparation, reaction, separation, and detection involved in the fields of biology and chemistry. On a chip of a few square centimeters (or even smaller), a network of microchannels is formed, and a controllable fluid runs through the entire system to replace various functions of conventional biological or chemical laboratories. So far, microfluidic chip technology has begun to be applied in different fields of life sciences. Recently, droplet-based microfluidics has attracted increasing attention. Compared with microchannels, microdroplets have the following...

AI Technical Summary

Problems solved by technology

[0004] (a) Observing micro-droplets by using a conventional CCD image sensor; the disadvantage of this method is: due to the limited sampling frame rate of CCD, it is difficult to directly observe high-throughput (high-speed motion) micro-droplets and obtain micro-droplets Information

[0005] (b) By using a high-speed camera to shoot the motion process of the micro-droplets in the microfluidic channel, the relevant information of the micro-droplets can be obtained t

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