A combined dynamic culture instrument for tissue engineering

Abstract

The invention relates to a combined type tissue engineering dynamic culture instrument. The combined type tissue engineering dynamic culture instrument comprises a plurality of culture instrument units, connecting lines and direct current power source receptacles, wherein each culture instrument unit comprises a box body, a driving motor, a rotary support base, an attraction block, a culture bottle and a rotor; two or more than two connecting holes are formed in the side surface of the box body; each direct current power source receptacle is detachably arranged in the corresponding connecting hole, and is respectively connected with the corresponding driving motor through a circuit, and the direct current power source receptacles are mutually connected by a circuit; the two ends of each connecting line are respectively connected with the corresponding culture instrument unit. The combined type tissue engineering dynamic culture instrument has the advantages that the structure is simple, the cost is lower, and the safety and reliability are realized; according to requirements, any number of culture instrument units can be combined; multiple culture instrument units share a power source adapter, and a built-in battery is not needed, so that the suitability is strong.

Description

technical field [0001] The invention relates to the field of biomedical tissue engineering, in particular to a combined dynamic culture instrument for tissue engineering. Background technique [0002] Tissue engineering is an emerging discipline that combines cell biology and material science to construct tissues or organs in vitro or in vivo. Its basic principle is to obtain a small amount of living tissue from the body, use special enzymes or other methods to separate cells (also known as seed cells) from the tissue for culture and expansion in vitro, and then combine the expanded cells with good biological phase. Capacitive, degradable, and absorbable biomaterials (scaffolds) are mixed in a certain proportion to allow cells to adhere to the biomaterials (scaffolds) to form a cell-material complex; a tissue or organ disease in which the complex is implanted into the body As the biomaterials are gradually degraded and absorbed in the body, the implanted cells continue to p...

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Problems solved by technology

In dynamic culture, it is usually necessary to use a dynamic incubator with stirring function and place it in an incubator under a sterile environment for cultivation. However, the current dynamic incubator is expensive, and it is difficult for laboratories in domestic universities , can not be purchased in batches; second, the volume is large, a standard incubator (inner diameter 46x46x48cm) can accommodate up to 4 instruments for a single seat, and 1 instrument for four seats. To give different mechanical stimuli to different tissues, multiple instruments are required. One incubator is difficult to implement. When using four-seat culture instruments, the stirring speed of the four seats is the same, which cannot meet the needs of experimental grouping; third, the base instrument cannot be sterilized by high temperature and high pressure, and it is easy to damage the culture environment; fourth, use 220V AC Power supply, after being placed in the incubator as a whole, the risk is high, the diameter of the wire is large, and it can only enter through the door crack of the incubator, destroying the closed and stable cultivation environment in the incubator, and accelerating the aging of the incubator; the fifth is the stirring of the culture solution The method is generally realized by the rotation of the stirring rod or the stirring blade inserted into the culture bottle, because the volume of the culture bottle is generally not large, and inserting the stirring rod or stirring blade will make the space in the culture bottle more cramped, so that it cannot accommodate larger engineering organization

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