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Aspergillus niger moldy bran culture medium and preparation method thereof

A technology of Aspergillus niger bran koji and culture medium, which is applied in the field of Aspergillus niger bran koji culture medium and its preparation, which can solve the problem of increased workload and labor costs for workers, prone to agglomeration of bran culture medium, and untimely loss of heat transfer, etc. problems, to achieve the effect of benefiting heat loss, improving growth quality and spore quantity, and high sterilization efficiency

Inactive Publication Date: 2016-11-23
RIZHAO JINHE BOYUAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the inherent characteristics of wheat bran, during the sterilization process, the bran medium is prone to agglomeration, which affects the sterilization effect. At the same time, the heat transfer and loss during the late Aspergillus niger bran culture process is not timely, resulting in the quality of the culture. However, screening out a large number of unqualified bran koji will bring hidden dangers to subsequent production, and increase the workload and labor costs for workers

Method used

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  • Aspergillus niger moldy bran culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Mix bran (particle size 0.3-6mm, moisture content 60%) and buckwheat husk (particle size 1-6mm) according to the weight ratio of 1:0.5, stir them evenly, add sulfuric acid to adjust the pH value to 6.0;

[0026] The above-mentioned mixed medium was divided into 14 bottles (each bottle contained 100 g), and then sterilized at a pressure of 0.14 MPa, a temperature of 121°C, and a constant temperature of 50 minutes to obtain the sterilized medium;

[0027] Take 10 of the bottles to observe the agglomeration situation and test the sterility; then the remaining 4 bottles are taken as the standard per gram of medium, and the number of Aspergillus niger spores inserted is 1×10 5 pcs / g. After culturing in a common culture environment in this field (environmental temperature 34°C, humidity 65%, flipping every 12 hours, culture for 10 days), the spores were counted. See Table 1 for detailed detection conditions.

Embodiment 2

[0029] Mix bran (particle size 0.3-6mm, moisture content 60%) and buckwheat husk (particle size 1-6mm) at a weight ratio of 1:1, stir them evenly, and then add sulfuric acid to adjust the pH value to 5.0;

[0030] The above-mentioned mixed medium was divided into 14 bottles (each bottle contained 100 g), and then sterilized at a pressure of 0.14 MPa, a temperature of 121°C, and a constant temperature of 50 minutes to obtain the sterilized medium;

[0031] Take 10 of them to observe the caking situation and test the sterility, then the remaining 4 bottles are taken as the standard per gram of medium, and the number of Aspergillus niger spores inserted is 2×10 6 pcs / g. The spores were counted under the common cultivation environment in this field (environmental temperature 37° C., humidity 50-70%, flipping every 12 hours, cultivation for 8 days). The detailed detection conditions are shown in Table 1.

Embodiment 3

[0033] Mix bran (particle size 0.3-6mm, moisture content 60%) and buckwheat husk (particle size 1-6mm) according to the weight ratio of 1:0.6, add citric acid to adjust the pH value to 7.0;

[0034] After it was stirred evenly, the above-mentioned mixed medium was divided into 14 bottles (each bottle contained 100 g), and then sterilized at a pressure of 0.14 MPa, a temperature of 121°C, and a constant temperature for 60 minutes to obtain the sterilized medium;

[0035] Take 10 of them to observe the caking situation, and test the sterility, and then the remaining 4 bottles are taken as the standard per gram of medium, and the number of Aspergillus niger spores inserted is 7×10 5 pcs / g. The spores were counted under the common culture environment in this field (environmental temperature 34°C, humidity 70%, retake every 12 hours, culture 11 days). The detailed detection conditions are shown in Table 1.

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Abstract

The invention relates to an aspergillus niger moldy bran culture medium and a preparation method thereof. The aspergillus niger moldy bran culture medium comprises bran and buckwheat hulls, the weight ratio of the bran to the buckwheat hulls is 1: (0.5-1.0), and the pH (potential of hydrogen) value of the culture medium is 5.0-7.0. The culture medium is high in sterilization efficiency and free from caking in the early sterilization process, heat generated by growth can be dissipated in the growth process of aspergillus niger, the growth quality and the spore amount of aspergillus niger moldy bran can be improved, and growth stability is ensured.

Description

technical field [0001] The invention relates to the field of strain cultivation, in particular to an Aspergillus niger bran koji culture medium and a preparation method thereof. Background technique [0002] Citric acid, scientific name 2-hydroxypropane-1,2,3-tricarboxylic acid, molecular formula C 6 h 8 o 7 (anhydrous), is an organic acid widely used in food, medicine and chemical industry. With the development of the economy, the demand for citric acid in all walks of life will rise steadily. Of course, the challenges and opportunities faced by various enterprises are also increasing. It is mainly used in food industry, pharmaceutical industry and chemical industry, and it is also widely used in industrial fields such as electronics, textiles, petroleum, leather, construction, photography, plastics, casting and ceramics. [0003] At present, in the citric acid industry, Aspergillus niger is the most commonly used citric acid fermentation strain, and the preparation of ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/685
CPCC12N1/14
Inventor 寇光智李昌涛刘春山蒋水星
Owner RIZHAO JINHE BOYUAN BIOCHEM
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