Semi-bionic extraction process for extracting rhodioloside from rhodiola crenulata

Abstract

The invention discloses a semi-bionic extraction process for extracting rhodioloside from rhodiola crenulata. On one hand, an extracting process of a semi-bionic extraction method accords with traditional Chinese medicine compatibility and clinical medicine characteristics and characteristics of translocation and absorption of oral medicines in gastrointestinal tracts. On the other hand, the loss of effective components is less, and the rhodioloside in the rhodiola crenulata can be extracted to the greatest extent. The effective components in the rhodiola crenulata are dissolved into an extracting solution and the extracting solution is stewed by adopting ethyl ether; the rhodioloside is not dissolved into the ethyl ether and impurity removal treatment can be carried out on the extracting solution, so that the purity of the extracting solution is improved. Moderately polar macroporous resin is used for eluting the extracting solution and then the extracting solution is concentrated, centrifuged and dried; finally, the high-purity rhodioloside is extracted to the greatest extent. A rhodioloside finished product with the yield of 1.6 percent to 2.1 percent and the content of 97 percent or above can be obtained.

Description

technical field

[0001] The invention belongs to the field of traditional Chinese medicine extraction, and in particular relates to a semi-bionic extraction process for extracting salidroside from rhodiola rosea. Background technique

[0002] Rhodiola daflora is the tuber of Rhodiola grandiflora, a plant of the Crassulaceae family, mainly produced in Tibet, northwestern Yunnan, and western Sichuan. Born in hillside grasslands, bushes, and rock crevices at an altitude of 2800-5600 meters. The functions and indications of Rhodiola daflora include: improving sleep, promoting blood circulation, anti-brain hypoxia, anti-fatigue, promoting blood circulation and stopping bleeding, clearing lung and relieving cough, reducing stasis and swelling, antipyretic and antipyretic, nourishing vitality, etc. Rhodiola daflora oral liquid is made by extracting the active ingredients of Rhodiola rosea, which is convenient to carry and take, and has high bioavailability. The main active ingredi...

Examples

Embodiment 1

[0039] A semi-bionic extraction process for extracting salidroside from Rhodiola grandiflora, characterized in that it comprises the following process steps:

[0040] A, raw material pre-treatment: take dry Rhodiola rosea, pulverize to obtain Rhodiola rosea powder;

[0041] B, extracting the Rhodiola grandiflora extract: get the Rhodiola grandiflora powder, and extract by using a semi-bionic extraction method to obtain the extract;

[0042] C. Add ether and let it stand: add ether to the extract, stir and let stand for at least 3 hours; filter after standing to obtain the filtrate;

[0043] D. Elution: The filtrate is adsorbed by a medium-polar macroporous resin, first washed with deionized water, and then eluted with a gradient of methanol, and the eluate is collected to recover methanol; after concentration, it is absorbed by a medium-polar macroporous resin, Rinse with deionized water, gradient elution with methanol, and collect the eluate;

[0044] E. Concentration, cent...

Embodiment 2

[0047] On the basis of Example 1, preferably, in step B of the present invention, the semi-bionic extraction process is carried out under airtight conditions, including the following steps:

[0048] Weakly acidic water extraction: Use weak acid to adjust the extraction water to a pH of 4.5-5.5, weigh Rhodiola rosea powder, add weakly acidic water according to the ratio of solid to liquid 1:12-1:20, and heat to 35 Extract at -45°C for 2-4 hours under reduced pressure, and separate to obtain filtrate 1 and filter residue 1.

[0049] Weak alkaline water extraction: Use a weak base to adjust the extraction water to a pH of 8.0-9.5, take the filtrate 1, add weak alkaline water according to the ratio of solid to liquid 1:8-1:10, and heat to 35- Extract at 45°C for 2-4 hours under reduced pressure, and separate to obtain filtrate 2 and filter residue 2.

[0050] Neutral water extraction: adjust the extraction water to neutral with a weak acid, take the filtrate 2, add neutral water ...

Embodiment 3

[0054] On the basis of Example 1, preferably, the weakly acidic water is made by passing carbon dioxide into the water; the weakly alkaline water is made by passing ammonia gas into the water.

[0055] In the present invention, carbon dioxide and ammonia are used to create a weakly acidic and weakly alkaline environment in the semi-bionic extraction, the acidity and alkalinity are weak, and the damage to the effective components of Rhodiola daflora is reduced. At the same time, acetic acid and ammonia are highly safe and volatile, and can reduce residues in the system after extraction.

technical field

[0001] The invention belongs to the field of traditional Chinese medicine extraction, and in particular relates to a semi-bionic extraction process for extracting salidroside from rhodiola rosea. Background technique

[0002] Rhodiola daflora is the tuber of Rhodiola grandiflora, a plant of the Crassulaceae family, mainly produced in Tibet, northwestern Yunnan, and western Sichuan. Born in hillside grasslands, bushes, and rock crevices at an altitude of 2800-5600 meters. The functions and indications of Rhodiola daflora include: improving sleep, promoting blood circulation, anti-brain hypoxia, anti-fatigue, promoting blood circulation and stopping bleeding, clearing lung and relieving cough, reducing stasis and swelling, antipyretic and antipyretic, nourishing vitality, etc. Rhodiola daflora oral liquid is made by extracting the active ingredients of Rhodiola rosea, which is convenient to carry and take, and has high bioavailability. The main active ingredi...

Examples

Embodiment 1

[0039] A semi-bionic extraction process for extracting salidroside from Rhodiola grandiflora, characterized in that it comprises the following process steps:

[0040] A, raw material pre-treatment: take dry Rhodiola rosea, pulverize to obtain Rhodiola rosea powder;

[0041] B, extracting the Rhodiola grandiflora extract: get the Rhodiola grandiflora powder, and extract by using a semi-bionic extraction method to obtain the extract;

[0042] C. Add ether and let it stand: add ether to the extract, stir and let stand for at least 3 hours; filter after standing to obtain the filtrate;

[0043] D. Elution: The filtrate is adsorbed by a medium-polar macroporous resin, first washed with deionized water, and then eluted with a gradient of methanol, and the eluate is collected to recover methanol; after concentration, it is absorbed by a medium-polar macroporous resin, Rinse with deionized water, gradient elution with methanol, and collect the eluate;

[0044] E. Concentration, cent...

Embodiment 2

[0047] On the basis of Example 1, preferably, in step B of the present invention, the semi-bionic extraction process is carried out under airtight conditions, including the following steps:

[0048] Weakly acidic water extraction: Use weak acid to adjust the extraction water to a pH of 4.5-5.5, weigh Rhodiola rosea powder, add weakly acidic water according to the ratio of solid to liquid 1:12-1:20, and heat to 35 Extract at -45°C for 2-4 hours under reduced pressure, and separate to obtain filtrate 1 and filter residue 1.

[0049] Weak alkaline water extraction: Use a weak base to adjust the extraction water to a pH of 8.0-9.5, take the filtrate 1, add weak alkaline water according to the ratio of solid to liquid 1:8-1:10, and heat to 35- Extract at 45°C for 2-4 hours under reduced pressure, and separate to obtain filtrate 2 and filter residue 2.

[0050] Neutral water extraction: adjust the extraction water to neutral with a weak acid, take the filtrate 2, add neutral water ...

Embodiment 3

[0054] On the basis of Example 1, preferably, the weakly acidic water is made by passing carbon dioxide into the water; the weakly alkaline water is made by passing ammonia gas into the water.

[0055] In the present invention, carbon dioxide and ammonia are used to create a weakly acidic and weakly alkaline environment in the semi-bionic extraction, the acidity and alkalinity are weak, and the damage to the effective components of Rhodiola daflora is reduced. At the same time, acetic acid and ammonia are highly safe and volatile, and can reduce residues in the system after extraction.

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