Method for measuring ciprofloxacin through graphene-and-hematoxylin-integrally-modified glassy carbon electrode

A graphene composite and glassy carbon electrode technology, which is applied in the direction of material electrochemical variables, etc., to achieve the effects of convenient and fast detection process, improved sensitivity, and environmental protection in the preparation process

Inactive Publication Date: 2017-01-04
SHANGHAI UNIV
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Problems solved by technology

The simultaneous detection of ciprofloxacin using a graphene-composite

Method used

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  • Method for measuring ciprofloxacin through graphene-and-hematoxylin-integrally-modified glassy carbon electrode
  • Method for measuring ciprofloxacin through graphene-and-hematoxylin-integrally-modified glassy carbon electrode
  • Method for measuring ciprofloxacin through graphene-and-hematoxylin-integrally-modified glassy carbon electrode

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Embodiment 1

[0024] The preparation method and steps of the modified glassy carbon electrode in the present embodiment are as follows:

[0025] (1) Pretreatment of the glassy carbon electrode: first, the glassy carbon electrode is coated with 0.05 μm Al 2 o 3 The polishing powder and suede are polished to a mirror surface, and then ultrasonically cleaned with distilled water, dilute nitric acid solution, absolute ethanol and double distilled water in sequence, and then used.

[0026] (2) Preparation of graphene-composite hematoxylin-modified glassy carbon electrode: first, graphene was dissolved in secondary water to prepare a suspension with a concentration of 1 mg / mL, and ultrasonicated for 30 min. A microsampler takes 300 μ in 10 mL of 0.1 mol / L KCl solution, and ultrasonically disperses for 30 minutes to obtain a graphene modification solution. Dissolve hematoxylin in 0.1mol / L (pH=7.0) phosphate buffer solution to prepare a hematoxylin modification solution with a concentration of 0....

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Abstract

The invention relates to a method for measuring ciprofloxacin through a graphene-and-hematoxylin-integrally-modified glassy carbon electrode, and belongs to the technical field of chemical measurement and electrochemical analysis detection. According to the method, graphene is subjected to electrolytic deposition, then the glassy carbon electrode modified by hematoxylin is subjected to electrochemical polymerization, and an even compounded modified film is formed in the surface of the electrode accordingly so that the stability and the sensitivity of the electrode can be improved. The sensitivity of the ciprofloxacin is subjected to quantitative analysis measurement with the square wave voltammetry and the current-time technology; the experimental result shows that in a 0.10-mol/L phosphoric acid buffering solution with pH equal to 3.0, the modified electrode has the obvious catalysis and sensitization effects on the ciprofloxacin; under the optimum condition, the current-time technology method is adopted for measurement, the ciprofloxacin and a peak current of the ciprofloxacin have the good linear relationship from 0.5 micron to 45.0 microns, and the detection limit is 0.20 micron. The method can be directly used for rapid electrochemical measurement of the ciprofloxacin, and has the advantages of being rapid, sensitive, accurate and the like.

Description

technical field [0001] The invention relates to a method for determining ciprofloxacin by a glassy carbon electrode modified by graphene composite hematoxylin, which belongs to the technical field of drug determination and electrochemical detection. Background technique [0002] Ciprofloxacin is a fluoroquinolone compound antibiotic drug. It is a synthetic third-generation quinolone antibacterial drug. It has broad-spectrum antibacterial activity and good bactericidal effect. It can kill bacteria such as anthrax, and cause DNA by interference. The enzyme that reverses transcription after replication prevents the synthesis of DNA and protein, that is, acts on the A subunit of DNA helicase in bacterial cells, inhibits DNA synthesis and replication, and leads to bacterial death. The antibacterial activity against almost all bacteria is 2-4 times stronger than that of norfloxacin and enoxacin. Has an antibacterial effect. Its antibacterial activity is the strongest among the w...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/30G01N27/48
Inventor 丁亚萍王英子李丽杨华崔世强廖兰凤
Owner SHANGHAI UNIV
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