United and quantitative anti-sperm antibody and Mullerian duct hormone detection card and manufacturing method and usage method thereof
An anti-sperm antibody and quantitative detection technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of inability to quantitatively detect anti-sperm antibodies, easily interfered by external factors, unable to accurately quantitatively detect, etc. Simple, fluorescently stable, and highly sensitive results
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[0043] The present invention will be described in detail below according to the accompanying drawings, which is a preferred embodiment of various embodiments of the present invention.
[0044] In a preferred embodiment, an anti-sperm antibody and anti-Müller tube hormone combined quantitative test card is made of polydimethylsiloxane (also called silicone elastomer or silicone rubber, PDMS) material, and is made of bottom It consists of a card and a face card. The bottom card structure includes a sample area 11 covered with filter paper, a binding area 12 coated with anti-sperm secondary antibodies and anti-Müllerian hormone antibodies labeled with different fluorescent microspheres, a parallel microchannel time valve 13, A detection area 14 coated with an anti-sperm secondary antibody and having a special structure, a detection area 15 and a waste liquid area 16 coated with an anti-Müllerian hormone antibody and having a special structure. The face card includes a sample hole an...
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