United and quantitative anti-sperm antibody and Mullerian duct hormone detection card and manufacturing method and usage method thereof

An anti-sperm antibody and quantitative detection technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of inability to quantitatively detect anti-sperm antibodies, easily interfered by external factors, unable to accurately quantitatively detect, etc. Simple, fluorescently stable, and highly sensitive results
CN106338601AActive Publication Date: 2017-01-18GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
Publication Date
2017-01-18

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Abstract

The invention relates to a united and quantitative anti-sperm antibody and Mullerian duct hormone detection card and a manufacturing method and a usage method thereof. The detection card comprises a bottom card and a face card, wherein the bottom card is provided with a sampling zone, a combining zone, a valve, a detection zone and a water liquid zone, the sampling zone is connected with the combining zone, the sampling zone and the combining zone are located at one end of the valve, the detection zone is connected with the waste liquid zone and is located at the other end of the valve, and the face card is provided with a sampling hole and a detection hole. The fluorescent microspheres used by the card are not affected by the outside environment, so that fluorescence is stable. In addition, the flowing speed of blood to be detected on the detection card is controlled through a microfluidic technology, and good conditions are provided for quantitative detection.
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Description

technical field

[0001] The invention relates to the technical field of the combination of microfluidic technology and immunofluorescence technology, in particular to an anti-sperm antibody and anti-Müllerian hormone combined quantitative detection card and its production and use methods. Background technique

[0002] In recent years, infertility caused by autoimmune disorders has attracted more and more attention. Antisperm antibody (AsAb) is the most common factor of immune infertility. Antisperm antibody (AsAb) is a complex pathological product. Its main functions are sperm agglutination and sperm braking, affecting sperm transport, inhibiting sperm penetration of cervical mucus, limiting sperm adhesion to egg zona pellucida, and inhibiting sperm acrosome The activity of sperm makes it difficult for sperm to penetrate the corona radiata and zona pellucida surrounding the egg cell, hinders the fusion of sperm and egg cell membranes, and prevents the combination of sperm and...

Claims

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