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United and quantitative anti-sperm antibody and Mullerian duct hormone detection card and manufacturing method and usage method thereof

An anti-sperm antibody and quantitative detection technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of inability to quantitatively detect anti-sperm antibodies, easily interfered by external factors, unable to accurately quantitatively detect, etc. Simple, fluorescently stable, and highly sensitive results

Active Publication Date: 2017-01-18
GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
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  • Application Information

AI Technical Summary

Problems solved by technology

This method cannot quantitatively detect the content of anti-sperm antibodies, and the sensitivity is low, and it is easily interfered by external factors
[0009] The double-antibody sandwich ELISA method can only qualitatively or semi-quantitatively detect the content of anti-Müllerian hormone in blood samples, but cannot accurately quantitatively detect

Method used

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  • United and quantitative anti-sperm antibody and Mullerian duct hormone detection card and manufacturing method and usage method thereof
  • United and quantitative anti-sperm antibody and Mullerian duct hormone detection card and manufacturing method and usage method thereof
  • United and quantitative anti-sperm antibody and Mullerian duct hormone detection card and manufacturing method and usage method thereof

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Embodiment Construction

[0043] The present invention will be described in detail below according to the accompanying drawings, which is a preferred embodiment among various implementations of the present invention.

[0044]In a preferred embodiment, a joint quantitative detection card for antisperm antibody and anti-Müllerian hormone is made of polydimethylsiloxane (also known as silicone elastomer or silicone rubber, PDMS) material, with a base Composed of a card and a surface card, the bottom card structure includes a sample loading area 11 covered with filter paper, a binding area 12 for anti-sperm secondary antibodies and anti-Müllerian hormone antibodies sprayed with different fluorescent microsphere labels, a parallel microchannel time valve 13, A detection area 14 coated with anti-sperm secondary antibody and having a special structure, a detection area 15 coated with an anti-Müllerian hormone antibody and having a special structure, and a waste liquid area 16 . The surface card includes a sam...

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Abstract

The invention relates to a united and quantitative anti-sperm antibody and Mullerian duct hormone detection card and a manufacturing method and a usage method thereof. The detection card comprises a bottom card and a face card, wherein the bottom card is provided with a sampling zone, a combining zone, a valve, a detection zone and a water liquid zone, the sampling zone is connected with the combining zone, the sampling zone and the combining zone are located at one end of the valve, the detection zone is connected with the waste liquid zone and is located at the other end of the valve, and the face card is provided with a sampling hole and a detection hole. The fluorescent microspheres used by the card are not affected by the outside environment, so that fluorescence is stable. In addition, the flowing speed of blood to be detected on the detection card is controlled through a microfluidic technology, and good conditions are provided for quantitative detection.

Description

technical field [0001] The invention relates to the technical field of the combination of microfluidic technology and immunofluorescence technology, in particular to an anti-sperm antibody and anti-Müllerian hormone combined quantitative detection card and its production and use methods. Background technique [0002] In recent years, infertility caused by autoimmune disorders has attracted more and more attention. Antisperm antibody (AsAb) is the most common factor of immune infertility. Antisperm antibody (AsAb) is a complex pathological product. Its main functions are sperm agglutination and sperm braking, affecting sperm transport, inhibiting sperm penetration of cervical mucus, limiting sperm adhesion to egg zona pellucida, and inhibiting sperm acrosome The activity of sperm makes it difficult for sperm to penetrate the corona radiata and zona pellucida surrounding the egg cell, hinders the fusion of sperm and egg cell membranes, and prevents the combination of sperm and...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/543G01N33/74G01N21/64
CPCG01N21/6402G01N21/6486G01N33/533G01N33/54313G01N33/74
Inventor 周辉
Owner GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
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