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Breeding method utilizing sodium azide to induce mutation of peanut

A sodium azide and peanut technology, which is applied in the directions of botanical equipment and methods, application, and plant genetic improvement, to achieve the effect of stable peanut traits and shortening the life span

Inactive Publication Date: 2017-02-15
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some scholars have explored the treatment of peanuts with the chemical mutagen EMS, but there are few reports on the treatment of peanuts with sodium azide

Method used

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  • Breeding method utilizing sodium azide to induce mutation of peanut
  • Breeding method utilizing sodium azide to induce mutation of peanut
  • Breeding method utilizing sodium azide to induce mutation of peanut

Examples

Experimental program
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Effect test

Embodiment 1

[0039] A breeding method utilizing sodium azide to mutate peanuts, comprising the following steps:

[0040] 1) Treat the peanut seeds: select 200 peanut seeds of the variety Yueyou 18 bred by the Crop Research Institute of the Guangdong Academy of Agricultural Sciences, wash with pure water and soak for 4 hours until fully imbibed, and then divide them into 4 groups in equal amounts. Groups were re-soaked in sodium azide solution with a concentration of 0, 1, 2, and 3 mol / L and treated in vacuum for 10 minutes. After the vacuum treatment, they continued to soak in sodium azide solution for 4 hours. , get M0 generation seed;

[0041] 2) Obtain the M1 generation seeds: dry the M0 generation seeds, then sow and raise seedlings, the emergence rate is as shown in Table 2, self-cross and single-plant harvest, and obtain the M1 generation seeds;

[0042] Table 2 The emergence rate of peanut seeds treated with different concentrations of sodium azide

[0043]

[0044] 3) Screenin...

Embodiment 2

[0055] A breeding method utilizing sodium azide to mutate peanuts, comprising the following steps:

[0056] 1) Treat the peanut seeds: select 200 peanut seeds of the variety Yueyou 13 bred by the Crop Research Institute of the Guangdong Academy of Agricultural Sciences, wash with pure water and soak for 4 hours until fully imbibed, and then divide them into 4 groups in equal amounts. Groups were re-soaked in sodium azide solution with a concentration of 0, 1, 2, and 3 mol / L and treated in vacuum for 15 minutes. After the vacuum treatment, they continued to soak in sodium azide solution for 4 hours. , get M0 generation seed;

[0057] 2) Obtain the M1 generation seeds: dry the M0 generation seeds, then sow and raise seedlings, the emergence rate is as shown in Table 5, self-cross and single-plant harvest, and obtain the M1 generation seeds;

[0058] Table 5 The emergence rate of peanut seeds treated with different concentrations of sodium azide

[0059]

[0060] 3) Screening ...

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Abstract

The invention discloses a breeding method utilizing sodium azide to induce mutation of peanut. The breeding method includes: treating peanut seeds; subjecting the treated peanut seeds to sowing and seedling raising to obtain M1-generation seeds; detecting and screening the M1-generation seeds, and then performing sowing and seedling raising to obtain M2-generation seeds. The breeding method specifically includes: 1), using pure water to clean and soak the peanut seeds till full imbibition, using a sodium azide solution to soak, performing vacuum treatment, and continuing using the sodium azide solution to soak after vacuum treatment is finished to obtain M0-generation seeds; 2), subjecting the M0-generation seeds to sowing and seedling raising to obtain the M1-generation seeds; 3), utilizing a near infrared detector to detect and analyze, and screening out target M1-generation seeds according to results of detection and analysis; 4), subjecting the target M1-generation seeds to sowing and seedling raising to obtain the M2-generation seeds. By the breeding method, crude fat content, total protein, oleic acid content and linoleic acid content of the peanut seeds can be increased.

Description

technical field [0001] The invention relates to a breeding method for using sodium azide to mutate peanuts, and belongs to the technical field of plant breeding. Background technique [0002] Peanut, also known as groundnut, is an annual herb and is an important oil and economic crop in my country. my country's peanut planting area and total production rank among the top in the world, and it has strong international competitiveness. However, the germplasm resources of peanuts in my country are relatively limited at present. The traditional peanut breeding time is too long, the efficiency is low and the shape is unstable. Due to serious globalization, it is necessary to improve peanut breeding through new technical means. More than half of the peanuts in our country are used for oil extraction. According to statistics, every 1% increase in peanut oil content will increase the economic benefits of enterprises by about 6%. Some scholars have explored the treatment of peanuts ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H1/04
CPCA01H1/06A01H1/04
Inventor 温世杰李杏瑜李少雄陈小平梁炫强
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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