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Immunoglobulin that binds the human vγ9vδ2 T cell receptor

An immunoglobulin and host cell technology, applied in the direction of anti-animal/human immunoglobulin, immunoglobulin, anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, etc., can solve unintentional activation, excessive Stimulus and other issues

Active Publication Date: 2021-05-04
LAVA THERAPEUTICS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to fighting disease, Vγ9Vδ2 T cells may be overstimulated or inadvertently activated in some diseases or medical treatments

Method used

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  • Immunoglobulin that binds the human vγ9vδ2 T cell receptor
  • Immunoglobulin that binds the human vγ9vδ2 T cell receptor
  • Immunoglobulin that binds the human vγ9vδ2 T cell receptor

Examples

Experimental program
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specific Embodiment approach

[0082] Generation of donor-derived Vγ9Vδ2 T cells

[0083] Healthy donor-derived (human) Vγ9Vδ2 T cells were generated and cultured as previously described (Schneiders FL, et al. Clin Immunol 2012; 142:194-200).

[0084] Generation of Jurkat Vγ9Vδ2 T-cell lines and Jurma Vγ9Vδ2 T-cell lines

[0085] Jurkat and JurMa cell lines expressing the Vγ9Vδ2 TCR were synthesized according to previously described methods (ScholtenKB, et al. Clin Immunol 2006; 119:135-45). Briefly, the protein sequences of cloned G9 Vγ9- and Vδ2-chains isolated by picornavirus-derived 2A sequences (Allison TJ et al. Nature 2001; 411:820-4, Davodeau F et al. Eur J Immunol 1993 ;23:804-8) were codon modified for optimal protein generation and synthesized by GeneART (Life technologies) followed by cloning into LZRS. After transfection into the Phoenix-A packaging cell line, retroviral supernatants were collected to transduce Jurkat or Jurma cells.

[0086] Selection of anti-Vγ9Vδ2 TCR VHH

[0087] 2 llamas...

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Abstract

The invention belongs to the field of medicine and relates to immunology, in particular to human Vγ9Vδ2 T cell receptor binding immunoglobulin molecules. Human Vγ9Vδ2 T cell receptor binding immunoglobulin molecules are especially useful in medical treatment and / or for detection of human Vγ9Vδ2 T cells, wherein human Vγ9Vδ2 T cells are regulatable.

Description

technical field [0001] The invention belongs to the field of medicine and relates to immunology. The present invention relates to immunoglobulins that bind T cells. In particular, the invention relates to immunoglobulins that bind to the human Vγ9Vδ2 T cell receptor. The invention provides an immunoglobulin molecule, such as an antibody, a single chain antibody or a single domain antibody, that binds a human Vγ9Vδ2 T cell receptor, wherein the human Vγ9Vδ2 T cell is regulatable. Background technique [0002] Most γδ peripheral blood lymphocytes (PBLs) in adults express a T-cell receptor (TCR) comprising Vγ9 and Vδ2 domains. Vγ9Vδ2 T cells can respond to a wide range of pathogens and tumor cells. This broad reactivity is understood to be conferred by phosphoantigens capable of specifically activating this T cell subset in a TCR-dependent manner. Broad-spectrum antimicrobial and antitumor reactivity of Vγ9Vδ2 T cells suggests direct involvement in immune control of cancer ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/28
CPCC07K16/2809C07K2317/70C07K2317/569C07K2317/22A61K2039/505C07K16/30C07K2317/31C07K2317/73C07K2317/75C07K2317/76A61P35/00C07K2317/622C07K2317/92C07K2317/20C07K2317/21C07K2317/565
Inventor 约翰内斯·耶勒·范德弗利特蕾妮·科妮莉亚·格拉里达·德布鲁恩坦尼亚·丹妮丝·德格鲁伊杰亨德里克·马里纳斯·威廉·费尔霍伊尔
Owner LAVA THERAPEUTICS BV