Application of substitutive 2,3-didetonindoline compound in preparing bacterial infection preventing and/or treating medicines
A technology of indolindione and bacterial infection, which is applied in the field of biomedicine and can solve problems such as unreported activity
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Embodiment 1
[0035] Example 1. The compound represented by formula I improves the survival rate of Caenorhabditis elegans infected by Enterococcus faecalis
[0036] 1. 54 hours before the nematode infection, the -80°C frozen Enterococcus faecalis (E.faecalis) was taken out of the refrigerator, streaked on the solid BHI medium, and cultivated at 37°C for 16 hours to obtain a single colony of Enterococcus faecalis.
[0037] 2. Inoculate the single colony of Enterococcus faecalis in step 1 into 5ml of BHI liquid medium, and culture on a shaker at 220rpm at 37°C for 14 hours.
[0038] 3. Take 100 μl of the Enterococcus faecalis cultured in step 2 and add it dropwise to the BHI solid plate medium and spread it evenly. After culturing at 25°C for 16 hours, turn to 15°C for 8 hours to obtain the Enterococcus faecalis BHI plate.
[0039] 4. 48 hours before nematode infection, place the L1-stage larvae of Caenorhabditis elegans of the same generation thermosensitive sterile type (glp-4:sek-1) on so...
Embodiment 2
[0049] Embodiment 2, the compound shown in formula I inhibits bacterial virulence gene expression
[0050] 1. Take the Enterococcus faecalis (E.faecalis) and Staphylococcus aureus (S.aureus) frozen at -80°C out of the refrigerator, streak them on solid BHI and TSB medium respectively, and culture them at 37°C for 16 hours Single colonies of E. faecalis and S. aureus were obtained.
[0051] 2. Inoculate the single clones of Enterococcus faecalis and Staphylococcus aureus in step 1 into 5ml of BHI and TSB liquid medium respectively, and cultivate them on a shaker at 37°C at 220rpm for 14 hours.
[0052]3. Take 100 μl of Enterococcus faecalis and Staphylococcus aureus cultured in step 2 and add it to 10ml of BHI and TSB liquid medium, culture on a shaker at 220rpm at 37°C for 5-6 hours, then add 200μl of the compound shown in formula Ⅰ at 10mg / ml Solution, continue to culture for 2 hours and then centrifuge the cells to extract the total RNA of Enterococcus faecalis and Staphylo...
Embodiment 3
[0057] Example 3. The compound represented by formula I regulates the expression of innate immune genes in Caenorhabditis elegans infected by Enterococcus faecalis
[0058] 1. 54 hours before the nematode infection, the -80°C frozen Enterococcus faecalis (E.faecalis) was taken out of the refrigerator, streaked on the solid BHI medium, and cultivated at 37°C for 16 hours to obtain a single colony of Enterococcus faecalis.
[0059] 2. Inoculate the single colony of Enterococcus faecalis in step 1 into 5ml of BHI liquid medium, and culture on a shaker at 220rpm at 37°C for 14 hours.
[0060] 3. Take 100 μl of the Enterococcus faecalis cultured in step 2 and add it dropwise to the BHI solid plate medium containing 20 μg / ml of the compound shown in formula Ⅰ and spread it evenly. After 16 hours of incubation at 25°C, turn to 15°C for 8 hours. Obtain E. faecalis BHI plates.
[0061] 4. 48 hours before the nematode infection, place the L1-stage larvae of the same-generation thermose...
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