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A method for tissue culture and rapid propagation of Pilocarpus tenifolia

A technology for the rapid propagation of Ilocarpus chinensis and tissue culture, which is applied in the field of plant tissue culture, can solve the problems of unstable output and short supply of Iocarpus chinensis, improve the reproduction coefficient and quality of seedlings, improve production efficiency, Effect of reduced incubation steps

Inactive Publication Date: 2018-05-08
三明市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a method for tissue culture and rapid propagation of Ilocarpus teniphyllum, the main purpose of which is to overcome the defects that the output of Ilocarpus tenifolia is not stable and the supply is often in short supply.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] step one

[0048] Selection of explants: cut semi-lignified vines of the vine of Pilocarpus chinensis, rinse them under tap water, and then cut the vines into 3-4 cm sections with a knot.

[0049] step two

[0050] Explant disinfection: Disinfect with 72-75% alcohol for 30-60 seconds under sterile conditions, then rinse with sterile water for 1-3 times; then disinfect with 0.1% mercury liter for 6-10 minutes, and then rinse with sterile water Rinse 3 to 5 times, and finally use sterile filter paper to dry the surface moisture for later use.

[0051] step three

[0052] Propagation and cultivation: Cut off the 0.5-1.0cm part of the two ends of the sterilized vine section that is in contact with the disinfectant, and inoculate the remaining vine section on the induction medium to obtain new seedlings; remove the leaves of the new seedlings, Cut into 1-3cm vine segments with nodules, spread them on the upper surface of the propagation solid medium, then implant 1 / 2-2 / 3 ...

Embodiment 2

[0064] step one

[0065] Selection of explants: cut semi-lignified vines of the vine of Pilocarpus chinensis, rinse them under tap water, and then cut the vines into 3-4 cm sections with a knot.

[0066] step two

[0067] Explant disinfection: Disinfect with 72-75% alcohol for 30-60 seconds under sterile conditions, then rinse with sterile water for 1-3 times; then disinfect with 0.1% mercury liter for 6-10 minutes, and then rinse with sterile water Rinse 3 to 5 times, and finally use sterile filter paper to dry the surface moisture for later use.

[0068] step three

[0069] Propagation and cultivation: Cut off the 0.5-1.0cm part of the two ends of the sterilized vine section that is in contact with the disinfectant, and inoculate the remaining vine section on the induction medium to obtain new seedlings; remove the leaves of the new seedlings, Cut into 1-3cm vine segments with nodules, spread them on the upper surface of the propagation solid medium, then implant 1 / 2-2 / 3 ...

Embodiment 3

[0081] step one

[0082] Selection of explants: cut semi-lignified vines of the vine of Pilocarpus chinensis, rinse them under tap water, and then cut the vines into 3-4 cm sections with a knot.

[0083] step two

[0084] Explant disinfection: Disinfect with 72-75% alcohol for 30-60 seconds under sterile conditions, then rinse with sterile water for 1-3 times; then disinfect with 0.1% mercury liter for 6-10 minutes, and then rinse with sterile water Rinse 3 to 5 times, and finally use sterile filter paper to dry the surface moisture for later use.

[0085] step three

[0086] Propagation and cultivation: Cut off the 0.5-1.0cm part of the two ends of the sterilized vine section that is in contact with the disinfectant, and inoculate the remaining vine section on the induction medium to obtain new seedlings; remove the leaves of the new seedlings, Cut into 1-3cm vine segments with nodules, spread them on the upper surface of the propagation solid medium, then implant 1 / 2-2 / 3 ...

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PUM

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Abstract

The invention relates to a method for tissue culture and rapid propagation of caulis piperis kadsurae. The method comprises the following steps: explant selection, explant sterilization, propagation and culture, rejuvenation, rooting and seedling integrated culture and test-tube plantlet transplantation. The method provided by the invention has the benefits that caulis piperis kadsurae seedlings are propagated through a plant tissue culture and rapid propagation technology, cannot be affected by seasons, substrates, scions and other factors and are produced throughout the year; a large quantity of caulis piperis kadsurae seedlings for field cultivation can be propagated within a shorter period of time; the caulis piperis kadsurae propagation coefficients and the seedling quality are obviously improved, and the production needs are met.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Chinese betelwort chinensis. Background technique [0002] Piper kadsura (Choisy) Ohwi is a plant of the family Peperaceae, mainly produced in Fujian, Guangdong, Hainan, Sichuan, Yunnan and Taiwan, etc. Ivy spinosa contains more volatile oils, has anti-inflammatory and analgesic pharmacological activities, and has the effects of dispelling rheumatism, dredging meridians, and relieving numbness. It is also widely used in medicated diet. [0003] Due to the good curative effect of Ivy chinensis, the amount of raw materials is constantly increasing, and the market is in short supply. In recent years, with the growing demand for Ipolocarpus spp. as snacks in Shaxian County, driven by economic interests, Yaonong has exhausted the wild resources, causing it to be on the verge of extinction. At present, the cuttings ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 周辉明林辉锋陈昌铭尚伟邓才生江秋萍夏朝水姚凤琴林发壮魏柳萍梁泉生
Owner 三明市农业科学研究院
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