Preparation method for multiplex immunocolloidal gold test strip used for apple latent viruses

An apple latent virus detection test paper technology, applied in the field of plant disease research, can solve the problems of complex operation, strong professional technology, and inability to meet the needs of field promotion and application, and achieve the effect of improving production quantity and product quality

Inactive Publication Date: 2017-04-26
山西师范大学
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  • Abstract
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  • Claims
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Problems solved by technology

The use of traditional serology, biology and molecular biology techniques to detect these viruses is complex, highly technical, and requires professional equipment, which cannot meet the needs of field promotion and application.

Method used

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  • Preparation method for multiplex immunocolloidal gold test strip used for apple latent viruses
  • Preparation method for multiplex immunocolloidal gold test strip used for apple latent viruses
  • Preparation method for multiplex immunocolloidal gold test strip used for apple latent viruses

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Embodiment Construction

[0035] The present invention will be further described below in conjunction with accompanying drawing:

[0036] The present invention comprises the following steps:

[0037] (1) Utensil cleaning:

[0038] Wash all the glassware to be used under running water, put them in acid solution (potassium dichromate 100g, concentrated sulfuric acid 50ml, add distilled water to 1000ml) and soak for 24h, rinse with tap water repeatedly, then soak in distilled water for 24h Wash with water three times, soak again in ultrapure water for 72 hours, dry in the oven for later use; be sure to maintain strict purity when preparing reagents, and use ddH for all reagents 2 0 preparation;

[0039] (2) Preparation of colloidal gold:

[0040] Take 1ml of 1% aqueous solution of chloroauric acid, add 99ml of ultrapure water, heat to boiling, quickly add 1.8ml of freshly prepared 1% trisodium citrate aqueous solution dropwise at one time while stirring, the color of the solution gradually turns from y...

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Abstract

The invention discloses a preparation method for a multiplex immunocolloidal gold test strip used for apple latent viruses. Compared with the prior art, the invention provides a novel means for rapid, specific and simple detection of main apple latent viruses. Research achievements in the invention allow the molecular variation, invasion prevalence and virulence status of latent viruses of apples produced in Shanxi Province to be determined; and the multiplex immunocolloidal gold test strip developed on the basis of the research achievements can accurately and rapidly diagnose whether a nursery stock or a rootstock carries a virus, so propagation and prevalence of a disease caused by the virus can be effectively controlled, and the output and quality of produced apples can be improved.

Description

technical field [0001] The invention relates to a method for studying plant diseases, in particular to a method for preparing an apple latent virus multiple immune colloidal gold detection test strip. Background technique [0002] Apple chlorotic leaf spot virus (Apple chlorotic leaf spot virus, ACLSV), apple stem pitting virus (Apple stem pitting virus, ASPV) and apple stem groove virus (Apple stem grooving virus, ASGV) have a very serious impact on apple yield and quality. These three types of latent viruses are widely distributed and have serious consequences. Using traditional serology, biology, and molecular biology techniques to detect these viruses requires complex operations, strong professional skills, and requires professional equipment, which cannot meet the needs of field promotion and application. Contents of the invention [0003] The object of the present invention is just to provide a kind of preparation method of apple latent virus multiple immune colloid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/532
CPCG01N33/56983G01N33/532
Inventor 陈伟樊泽璐王圣楠杨延祯蔡华成王祎玲郭瑞珍
Owner 山西师范大学
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