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Method for carrying out detoxification and rapid propagation by virtue of garlic rachis and application of method

A technology of inflorescence rachis and garlic, which is applied in the field of agricultural bioengineering, can solve the problems of difficult domestication of plants, time-consuming and labor-consuming, application limitations, etc., and achieve the effects of ensuring genetic stability, reducing production costs, and saving cultivation time

Active Publication Date: 2017-05-10
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Many researchers obtained a large number of regenerated plants from callus using in vitro culture technology, but lost a large number of plants in the process of rooting induction and transplanting, which reduced the reproduction efficiency, was time-consuming and labor-intensive, and was limited in application
Some researchers have improved the survival rate of garlic regenerated plants by optimizing the transplanting conditions, but the plant domestication is difficult and the effect is not ideal

Method used

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  • Method for carrying out detoxification and rapid propagation by virtue of garlic rachis and application of method
  • Method for carrying out detoxification and rapid propagation by virtue of garlic rachis and application of method
  • Method for carrying out detoxification and rapid propagation by virtue of garlic rachis and application of method

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Experimental program
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Embodiment 1

[0039] (1) Induction of adventitious buds on inflorescence rachis: choose Baodi garlic "Liupethong", when the garlic enters the reproductive growth period, when the flower stem:pseudostem = 1-1.2, pick the inflorescence rachis of garlic and store at 4°C for two weeks. Rinse the inflorescence rachis after two weeks of low-temperature storage under tap water for 30 min with 0.1% HgCl 2 Disinfect for 20 min, rinse with distilled water for 3 times, then disinfect with 75% alcohol for 10 min, and rinse with distilled water for 3 times. Peel off the outer bract leaves and the degenerated flower primordium residues and membranous bracts on the surface, and remove the flower stem part, insert the inflorescence rachis into the adventitious bud induction medium, and insert 2 inflorescence rachis into each bottle. The medium for inducing adventitious buds is: MS+2.0mg L -1 KT+0.1mgL -1 NAA+ 30.0 g·L -1 Sucrose+7.5 g·L -1 Agar, pH=6.2. The number of adventitious buds induced by ea...

Embodiment 2

[0047] 1. Induction of adventitious buds induced by garlic inflorescence rachis

[0048] Since different hormone types (KT, 6-BA, and NAA) and concentrations have certain effects on the regeneration efficiency of adventitious buds, we designed 12 kinds of screening media, and inserted the garlic flower axes after two weeks of low temperature storage into the above screening media Among them, the adventitious bud induction rate was counted to determine the best induction culture conditions, and the results are shown in the following table:

[0049] Table 2 Effects of different hormone ratios on the proliferation of adventitious buds on inflorescence axis of Baodi garlic

[0050]

[0051] It can be seen from Table 2 that among the medium 1 to 6, the induction rate of the medium 2 is higher, and the number of adventitious buds induced by each inflorescence axis reaches 6.12. When the concentration of 6-BA was the same, the induction rate of the medium without adding NAA was h...

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Abstract

The invention discloses a method for carrying out detoxification and rapid propagation by virtue of a garlic rachis. The method mainly comprises the following steps: inducing adventitious buds into test tube bulbs by virtue of garlic rachises as an explant, carrying out low-temperature domestication on the test tube bulbs, and planting the test tube bulbs into soil, so as to obtain a complete system of a detoxified seed garlic, wherein the number of the adventitious buds induced by each garlic rachis can reach 11, a culture medium for inducing the test tube bulbs is MS+120g.L<1>soft sugar+6.8g.L<1>agar, and pH is 7.5. According to the method, the garlic rachises of Baodi garlic-''Liubanhong'' are taken as the explant, a complete garlic detoxification and rapid propagation method is constructed through technical links of high-frequency induction of the test tube bulbs, the low-temperature domestication of the test tube bulbs, the optimization of planting conditions and the like, the induction rate of the test tube bulbs induced by the adventitious buds can be as high as 98.78%, the number of the adventitious buds induced by each garlic rachis can be as high as 10.64, and the propagation coefficient is increased by above two times relative to that of a direct planting manner of the bulbs; technical supports are provided for the improvement of the detoxification and rapid propagation efficiencies of the garlic, the saving of the cultivation time and the lowering of the production cost.

Description

[0001] The invention was supported by the key project of Tianjin Agricultural Committee (201302030); the key project of Tianjin Normal University Applied Development Research Fund (52XK1210, 52XK1505). technical field [0002] The invention belongs to the technical field of agricultural bioengineering, and relates to methods for tissue culture and rapid propagation of garlic, more specifically, a new method for detoxification and rapid propagation of garlic inflorescence rachis. Background technique [0003] garlic( Allium sativum L. ) is an annual plant of the genus Allium in the family Liliaceae. Most of the garlic cultivars cannot form seeds, and they usually reproduce asexually. This limits the biodiversity and genetic characteristics of garlic, causes serious species degradation, and seriously affects the yield and quality of garlic. Long-term asexual reproduction also leads to the continuous accumulation of garlic viruses, seriously affecting the growth of garlic plan...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王振英范宝莉尚云涛徐李薇刘晓颖党光兰桂霞
Owner TIANJIN NORMAL UNIVERSITY
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