Hydroponic domestication method of euonymus japonicus tissue culture seedlings

A technology of tissue culture seedlings and hydroponics, applied in botany equipment and methods, cultivation, culture medium, etc., can solve the problems of slow speed, low efficiency, and easy death of tissue culture seedlings during hardening, so as to promote rapid vegetative growth , the effect of shortening the rearing period

Inactive Publication Date: 2017-05-10
河南红枫生物高科股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, traditional methods such as cuttings and grafting are mostly used in production, which is slow and inefficient.
In recent years, tissue culture and rapid propagation techniques have been tried, but the tissue culture seedlings are prone to death during the hardening stage, and the survival rate of transplanting is extremely low, only reaching 30% to 45%, which hinders the production of Xuezhonghong, a new variety of colorful leaf plants. develop

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The implementation site is the Yellow River Beach Base of Henan Red Maple Seedlings in Wuzhi County, Jiaozuo County, Henan Province, and the time is spring March 2014. The specific steps are as follows:

[0029] 1. Open the lid and refine the seedlings:

[0030] Select tissue culture bottle seedlings with robust plants and differentiated roots, and transfer them from a constant temperature, sterile tissue culture room to an artificial climate room with an average temperature of 3-14℃, a maximum light intensity of 8000-10000Lx, and an average humidity of 75%, without opening them After 14 days of slowing the seedlings in the bottle, add 0.15% potassium permanganate or 1g / L thiophanate methyl and then open the cap to refine the seedlings for 7 days;

[0031] 2. Air cultivation to induce roots:

[0032] After opening the lid and refining the seedlings, add appropriate amount of warm water to the tissue culture seedlings, wash the root culture medium and pick them out separately, ...

Embodiment 2

[0040] The implementation site was Xinzheng Base of Henan Red Maple Seed Co., Ltd., Zhengzhou City, Henan Province, on June 3, 2014. The specific steps are as follows:

[0041] 1. Open the lid and refine the seedlings:

[0042] Choose tissue culture bottle seedlings with robust plants and differentiated roots, and transfer them from a constant temperature, sterile tissue culture room to a shade canopy with an average temperature of 20-31°C, a maximum light intensity of 10000-15000Lx, and an average humidity of 85%, without opening After bottled seedlings for 12 days, add 0.15% potassium permanganate or 1g / L thiophanate methyl and then open the cap to refine the seedlings for 7 days;

[0043] 2. Air cultivation to induce roots:

[0044] After opening the lid and smelting the seedlings, add appropriate amount of warm water to the tissue culture seedlings, wash the root medium and pick them out separately, soak them with 1000ppm carbendazim for 3 minutes, wrap them with sterilized sponge...

Embodiment 3

[0052] The implementation site is the east base of Henan Red Maple Seedling Co., Ltd., Zhengzhou City, Henan Province, on September 1, 2014. The specific steps are as follows:

[0053] 1. Open the lid and refine the seedlings:

[0054] Select tissue culture bottle seedlings with robust plants and differentiated roots, and transfer them from a constant temperature and sterile tissue culture room to an average temperature of 16-27°C, a maximum light intensity of 9000-11000Lx, and an average humidity of 70% in a grass-covered arch shed without opening them. After 15 days of slowing the seedlings in the bottle, add 0.15% potassium permanganate or 1g / L thiophanate methyl and then open the cap to refine the seedlings for 7 days;

[0055] 2. Air cultivation to induce roots:

[0056] After opening the lid and consolidating the seedlings, add appropriate amount of warm water to the tissue culture seedlings, wash the root medium and pick them out separately, soak them with 1000ppm chlorothaloni...

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PUM

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Abstract

The invention relates to the technical field of plant cultivation, and provides a hydroponic domestication method of euonymus japonicus tissue culture seedlings. The hydroponic domestication method comprises the following steps: uncovering for hardening the seedlings, performing aerial cultivation for inducing rooting, hydroponic cultivation for promoting growth, and performing domestication management. According to the hydroponic domestication method provided by the invention, the transplanting survival rate of the euonymus japonicus tissue culture seedlings reaches up to 85% or higher, rapid vegetative growth of the tissue culture seedlings is obviously promoted, and the nursing period of commercial seedlings is effectively shortened.

Description

Technical field [0001] The invention relates to the technical field of plant cultivation and cultivation, in particular to a method for hydroponic domestication of snow red tissue culture seedlings. Background technique [0002] Although the tissue cultured seedlings have certain photosynthesis ability, they have been exposed to high humidity, low light and low CO. 2 , Growing under constant temperature and heterotrophic conditions, so the tissue differentiation is not perfect, the photosynthetic autotrophic ability is weak, the adaptability is poor, the stomata are many and not easy to close, the chlorophyll synthesis is low, and the root hairs are sparse. The purpose of refining seedlings is to gradually improve the growth of their tissues by gradually changing their growth conditions, so as to finally adapt to the external natural conditions and environment. Life, in general, is to gradually reduce the temperature, increase the temperature difference, reduce the humidity, and i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00C05G3/00A01G31/00
CPCA01G24/00A01G31/00C05B7/00C05G3/00C05G5/23C05C3/00C05C5/02C05C5/04C05D1/02C05D5/00C05D9/00C05F11/10C05D9/02
Inventor 张丹张茂邓坤王东超刘少君
Owner 河南红枫生物高科股份有限公司
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