A group of peptides and their pharmaceutical compositions and applications
A composition and drug technology, applied in the field of peptides or their pharmaceutically acceptable salts, preparation of pharmaceutical compositions, and new peptides, can solve problems such as difficult folding, limited production, cost and production bottlenecks
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[0056] 1. Experimental animals, instruments and reagents
[0057] Adult Sprague-Dawley male rats (more than 8 weeks old), weighing 180g-220g, were purchased from Shandong Jinan Pengyue Experimental Animal Breeding Co., Ltd.
[0058] Carrageenan (Carrageenan, abbreviated as Carr) is a product of the U.S. sigma company, and 1% carrageenan is prepared respectively (0.1g carrageenan is dissolved in 10ml 0.9% normal saline solution, and is divided into 10 EP tubes) and 2% carrageenan (0.2g carrageenan dissolved in 10ml 0.9% normal saline solution, distributed to 10 EP tubes), stored at -20°C, taken out half an hour before use and placed on ice.
[0059] GsMTx-4 was purchased from alomone labs in the United States. All peptides from SEQ ID No.1 to SEQ ID No.8 were prepared into 5mM or 10mM mother solutions with high-temperature sterilized deionized water (DD water), and distributed in EP tubes. Store at -80°C and dilute with saline to the concentration required for the experiment w...
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