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Bacillus safensis YJC-4 and application thereof to prevention and control of tobacco black shank and growth promotion

A technology of bacillus saphobia and YJC-4, applied in the application, pest control, plant growth regulator and other directions, can solve the problem of insufficient control of tobacco black shank, and achieve difficult drug resistance, good biocontrol effect, and good The effect of antagonistic activity

Active Publication Date: 2017-05-10
中国烟草总公司海南省公司 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is also commercialized Bacillus subtilis at present, but there are still deficiencies in the prevention and treatment of tobacco black shank and growth promotion

Method used

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  • Bacillus safensis YJC-4 and application thereof to prevention and control of tobacco black shank and growth promotion
  • Bacillus safensis YJC-4 and application thereof to prevention and control of tobacco black shank and growth promotion
  • Bacillus safensis YJC-4 and application thereof to prevention and control of tobacco black shank and growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Screening of Bacillus shamfu bacillus YJC-4 and its antagonism to black shank bacteria

[0045] 1.1 Isolation and screening of antagonistic bacteria:

[0046] A total of 87 soils were collected from Kaiyang, Qingzhen, Guizhou, Panzhihua, Xichang, Sichuan, Zhucheng, Yishui, Jimo, Shandong, and other places. In order to obtain highly effective antagonistic bacteria, the root soil of black shank diseased plants at a depth of 5-10cm and the adjacent soil were selected. The root soil of healthy plants was marked and stored in a -20°C refrigerator until use.

[0047] The 87 parts of soil collected were treated as follows: firstly, the roots, stems, leaves and other sundries in the soil were removed, crushed and passed through a 20-mesh sieve. Weigh 5g of the treated soil with an electronic balance, put it into a 100mL Erlenmeyer flask, add water to make up to 50mL, shake evenly, take 1mL of the supernatant and dilute to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -...

Embodiment 2

[0072] The pot experiment of embodiment 2 shamfu bacillus YJC-4 preventing and treating tobacco black shank

[0073] Take three potted plants of Safflower Dajinyuan tobacco plants that have grown to 5-6 true leaves, and inoculate them with black shank bacteria under the same conditions: gently scratch the roots of the tobacco plants with a knife, and inoculate the cultivated black shank bacteria Inoculate into the wound.

[0074] Figure 8 Shown are the growth conditions of tobacco plants with three different treatments: No. 1 is after the inoculation of black shank bacteria, and the roots are immediately irrigated with 20mL (2±0.5)×10 6 The growth status of cfu / mL YJC-4 antagonistic bacteria 7 days after inoculation; No. 2 is 3 days after the inoculation of black shank bacteria, and the disease status of tobacco plants without YJC-4 antagonistic bacteria; No. 3 is 7 days after inoculation of black shank bacteria , No use of YJC-4 antagonists, the disease status of tobacco p...

Embodiment 3

[0075] Example 3 Pot experiment on the prevention and treatment of tobacco black shank by Bacillus shamfu bacillus YJC-4

[0076] Shake cultured YJC-4 bacteria solution, the concentration of the bacteria solution is about (2±0.5)×10 8 cfu / mL, after diluting 100 times, root-irrigate the potted tobacco plants that have grown to 5-6 true leaves, and at the same time inoculate the tobacco plants with blackleg bacteria under the same conditions: scratch lightly with a knife Injure the roots of tobacco plants, and inoculate the cultured blackleg bacteria into the wound. The medicine was applied every 7 days for a total of 3 times. The control agent is sprayed with 58% nail cream · manganese zinc wettable powder and 100 billion / g Bacillus subtilis powder respectively 500 times liquid to inoculate the tobacco strain of black shank bacteria, and the blank control is treated with an equal amount of sterile water, and the others are the same. See Table 2 for specific operations. 20 pl...

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Abstract

The invention provides bacillus safensis YJC-4. The class name is bacillus safensis and the bacillus safensis YJC-4 is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation date is April 15, 2016, the preservation address is #3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.12356. The strain has the following advantages that (1) the bio-control effect is good; in a pot culture experiment, YJC-4 bacterium liquid is used for treating tobacco plants inoculated with tobacco black shank bacteria and the prevention and control effect reaches 81.59 percent and is remarkably better than that of 58 percent metalaxyl.mancozeb and a biological preparation, namely bacillus subtilis; the bacillus safensis YJC-4 has a potential application value in the aspect of biological prevention and control of the tobacco black shank and has good development and application prospects; the biological prevention and control is environment-friendly, does not easily have drug resistance and is safe to people and livestock, and conditions are created for green and prevention and control development of tobaccos; (2) the growth promoting effect is good; the biological amount and agronomic characters of YJC-4 bacterium liquid tobacco plants are remarkably promoted and the promoting effect is remarkably different from a control group.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to a strain of Bacillus suffolus YJC-4 and its application in preventing and controlling tobacco black shank and promoting growth. Background technique [0002] Tobacco black shank (Phytophthora parasitica var.nicotianae) is a facultative parasite, which can induce tobacco black shank (Tobacco Black Shank), and has devastating damage to tobacco. Black shank bacteria like high temperature and high humidity. When the temperature is 28-30 ℃ and the humidity reaches above 80%, it will create favorable conditions for the release of sporangia and the spread of zoospores. At this time, it is most likely to cause the epidemic of tobacco black shank And occur, causing a large area of ​​tobacco plant death. Tobacco, as its host plant, reaches its peak during the period from the cluster stage to the vigorous growth period, and mainly damages the roots and stem bases of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 李锡宏刁朝强陈德鑫徐同伟简胜义杨艺炜文锦涛张晓强黄翔聂忠扬
Owner 中国烟草总公司海南省公司
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