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Application of Shp2 protein in preparation of kit for evaluating therapeutic effect of sorafenib on liver cancer

An efficacy evaluation and kit technology, which is applied in the application field of preparing a kit for evaluating the efficacy of sorafenib for liver cancer, and can solve problems such as no research report on Shp2 protein.

Inactive Publication Date: 2017-05-10
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether the prognosis of patients with low expression of Shp2 is different compared with those without sorafenib, that is to say, whether patients with low expression of Shp2 are sensitive to sorafenib has not yet been reported
[0009] At present, there is no research report on the application of Shp2 protein in the preparation of liver cancer Sorafenib efficacy evaluation kit

Method used

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  • Application of Shp2 protein in preparation of kit for evaluating therapeutic effect of sorafenib on liver cancer
  • Application of Shp2 protein in preparation of kit for evaluating therapeutic effect of sorafenib on liver cancer
  • Application of Shp2 protein in preparation of kit for evaluating therapeutic effect of sorafenib on liver cancer

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Experimental program
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Effect test

Embodiment 1

[0060] Collect 77 liver cancer tissues of patients taking sorafenib postoperatively and 71 liver cancer tissues of patients who did not take sorafenib postoperatively (the liver cancer tissues were all from the Eastern Hepatobiliary Surgery Hospital and were diagnosed as hepatocellular carcinoma by 2 pathologists ), using immunohistochemical methods to detect the expression of Shp2 protein in liver cancer tissues and calculate the immunohistochemical score, the specific steps are as follows:

[0061] (1) Prepare paraffin sections of liver cancer tissue, oven at 60℃ overnight;

[0062] (2) Dewax slices to water;

[0063] (Xylene I①10min→Xylene II②10min→Xylene III③10min→100% ethanol 5min→95% ethanol 5min→85% ethanol 5min→75% ethanol 5min→double distilled water 5min)

[0064] (3) 3%H 2 O 2 The solution is kept at room temperature for 20 minutes;

[0065] (4) Double-distilled water washing for 5min×3;

[0066] (5) Antigen retrieval: Put the slices into 0.01M citrate buffer (pH 6.0) and boil...

Embodiment 2

[0084] The experimental steps of the above immunohistochemical method were used to detect the expression of Shp2 protein in the paraffin sections of liver cancer tissues of 77 patients taking sorafenib after the operation, and the expression level of tumor tissue was determined according to the computer image processing software, and the patients were classified according to the expression. Divided into high and low expression groups (see figure 2 ).

Embodiment 3

[0086] Liver cancer patients were divided into groups according to the expression of Shp2 protein, SPSS software 18.0 was used for data analysis, Kaplan-Meier method was used for survival curve analysis, and log-rank test was used for comparison between high and low groups. The results showed that in patients with low Shp2 expression, the medication group had a better prognosis than the control group, and there was a statistical difference in survival analysis between the two groups (P=0.41). The results suggest that patients with low Shp2 expression have a good response to sorafenib and are suitable for sorafenib treatment. see image 3 .

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Abstract

The invention belongs to the technical field of biology. The expression quantity of Shp2 protein in liver cancer tissues is detected by adopting an immunohistochemical method so as to determine the sensitivity of liver cancer patients on sorafenib. The invention provides an application of Shp2 protein in the preparation of a kit for evaluating the therapeutic effect of sorafenib on liver cancer. The Shp2 protein is used as a protein molecular marker for determining the therapeutic effect of sorafenib on the liver cancer, and has an important guiding significance for a liver cancer patient as whether to use sorafenib for treatment or not.

Description

Technical field [0001] The invention relates to the field of biotechnology, and relates to an application of Shp2 protein, specifically, the application of Shp2 protein in preparing a kit for evaluating the therapeutic effect of liver cancer on sorafenib. Background technique [0002] Primary liver cancer is one of the most common malignant tumors in the world, among which hepatocellular carcinoma (HCC) accounts for about 90%. According to the "Global Latest Cancer Statistics Release" data, there were 782,000 new cases and 745,000 deaths worldwide in 2012. Among them, the number of new cases and deaths in China accounted for about half, and primary liver cancer has become the second leading cause of death in China. [0003] In recent years, the application of molecular targeted drugs in the treatment of liver cancer has gradually received attention and is becoming a new hot spot. The multi-target receptor tyrosine kinase inhibitor sorafenib (Nexavar) is currently the only drug ap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/577
CPCG01N33/57438G01N33/577
Inventor 丁劲王红阳李晓峰向代民程卓
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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