Device for detecting DNA copy number variation of circulating tumor

A tumor and data technology, applied in the field of devices for detection of DNA copy number variation in circulating tumors, can solve the problems of increasing the difficulty of detection and the low proportion of ctDNA

Active Publication Date: 2017-05-10
ANNOROAD GENE TECH BEIJING +2
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Problems solved by technology

In addition, the proportion of ctDNA carrying CNV mutations released by tu

Method used

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  • Device for detecting DNA copy number variation of circulating tumor
  • Device for detecting DNA copy number variation of circulating tumor
  • Device for detecting DNA copy number variation of circulating tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081]The CNV situation of a peripheral blood sample of a female breast cancer patient is detected by using the device for detecting copy number variation of a circulating tumor DNA sample of the present invention.

[0082] 1.1 Extraction of cfDNA from peripheral blood samples

[0083] Blood cfDNA was extracted with MagMAX Cell-Free DNA Isolation Kit (Life Company) to obtain the extracted cfDNA, and the extraction method was referred to the user manual.

[0084] 1.2 End Repair

[0085] (1) Take out the required reagents from the kit stored at -20°C in advance. See Table 1 for the preparation amount of a single sample.

[0086] Table 1

[0087]

[0088] (2) End-repair reaction: After adding the DNA sample, place a 1.5 mL centrifuge tube in a Thermomixer for 30 minutes at 20°C. After the reaction, 1.8× nucleic acid purification magnetic beads were used to recover the DNA in the purification reaction system and dissolved in 32 μL EB.

[0089] 1.3 Add "A" at the end (A-Tail...

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Abstract

The invention relates to a device for detecting DNA copy number variation of circulating tumor, which has high detection sensitivity. The device provided by the invention comprises a sequencing data acquisition module, a sequence matching module, an early data processing module, a normalization module, a background library screening module, a data fluctuation eliminating module, a GC correction module and an output module.

Description

technical field [0001] The invention belongs to the field of molecular biology detection, and in particular relates to a device and a method for detection of circulating tumor DNA copy number variation. Background technique [0002] As early as 1948, Mandel and Metais detected free DNA fragments (cell-free, cfDNA) from normal human blood. In 1977, Leon et al. found that the content of cfDNA in tumor patients was significantly higher than that in healthy individuals, and the content of cfDNA in patients with advanced tumors was higher. With the deepening of the research, the researchers found that in the plasma and serum cfDNA of tumor patients, there is a DNA fragment identical to that of the tumor gene, which is named ctDNA (circulating tumor DNA). ctDNA is the genomic DNA released by tumor cells into the peripheral blood. Due to the short half-life of circulating DNA in peripheral blood, circulating tumor DNA can truly reflect the true situation of gene mutations in dise...

Claims

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Application Information

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IPC IPC(8): G06F19/20
CPCG16B25/00
Inventor 荆瑞琳张萌萌陈利斌王晓雯陈玉洁玄兆伶李大为梁峻彬陈重建
Owner ANNOROAD GENE TECH BEIJING
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