Beautiful millettia root tissue culture seedling raising method

A tissue culture and powerful technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficult industrialization, poor quality, many split ends, etc., to achieve tidy growth, convenient material acquisition, and excellent properties. Effect

Inactive Publication Date: 2017-05-24
黄振忠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are mainly low seedling rate, only 40% to 55%, many forks, slow growth, low yield, poor quality, and plants that...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: a kind of vigorous tissue culture seedling cultivation of cattle, the concrete steps are as follows:

[0017] 1. Seed collection: choose a strong, disease-free wild cattle plant as the mother plant for seed collection. After the frost solar term passes, the seeds are mature, and the seeds are collected;

[0018] 2. Cultivate aseptic seedlings: Soak and sterilize Niu Dali seeds in 0.3% mercuric chloride solution for 20 minutes, soak them with sterile water on the ultra-clean workbench for 3 times, each time for 10 minutes, and then sow the seeds on The culture medium was cultivated under natural scattered light indoors;

[0019] 3. Prepare induction medium: use MS as the basic medium, add plant hormone benzylpurine (BA) for induction culture, the concentration is 1.5mg / L, add 3% sucrose and 0.6% agar at the same time, and maintain the temperature of the medium 28°C, pH 5.5;

[0020] 4. Cluster bud induction: when the sterile seedlings cultivated in step 2...

Embodiment 2

[0024] Embodiment 2: a kind of vigorously tissue-cultured seedling cultivation of cattle comprises the following steps:

[0025] 1. Seed collection: choose a strong, disease-free wild cattle plant as the mother plant for seed collection. After the frost solar term passes, the seeds are mature, and the seeds are collected;

[0026] 2. Cultivate sterile seedlings: Soak and disinfect the seeds of Niu Dali in 0.3% mercuric chloride solution for 22 minutes, soak them with sterile water on the ultra-clean workbench for 3 times, each time for 10 minutes, and then sow the seeds on The culture medium was cultivated under natural scattered light indoors;

[0027] 3. Preparation of induction medium: use MS as the basic medium, add plant hormone benzylpurine (BA) for induction culture, the concentration is 2.0mg / L, add 3% sucrose and 0.7% agar at the same time, and maintain the temperature of the medium 29°C, pH 5.6;

[0028] 4. Induction cluster induction: when the sterile seedlings cu...

Embodiment 3

[0032] Embodiment 3: a kind of vigorous tissue culture seedling cultivation of cattle comprises the following steps:

[0033] 1. Seed collection: choose a strong, pest-free wild cattle plant as the mother plant for seed collection. After the frost solar term passes, the seeds are mature, and the seeds can be collected;

[0034] 2. Cultivate aseptic seedlings: Soak and sterilize Niu Dali seeds in 0.3% mercuric chloride solution for 25 minutes, soak them in sterile water on an ultra-clean workbench for 3 times, each time for 10 minutes, and then sow the seeds in The culture medium was cultivated under natural scattered light indoors;

[0035] 3. Prepare induction medium: use MS as the basic medium, add plant hormone benzylpurine (BA) for induction culture, the concentration is 2.5mg / L, add 3% sucrose and 0.8% agar at the same time, the medium temperature is maintained 30°C, pH 5.8;

[0036] 4. Cluster bud induction: when the sterile seedlings cultivated in step 2 grow to 8 cm,...

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PUM

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Abstract

The invention discloses a beautiful millettia root tissue culture seedling raising method, belonging to the technical field of traditional Chinese medicinal materials culture. The method comprises the following steps: collecting seeds, culturing sterile seedlings, preparing an induced culture medium, inducing clustered shoots, carrying out subculture proliferation on the clustered shoots, inducing rooting and hardening seedlings. The method effectively overcomes the defects of low planting percent, low growth speed, low yield, poor quality and difficulty in implementing industrialization. The method is convenient for taking materials; the inductivity of clustered shoots reaches 65-80%; the planting percent is up to 85% or above; and the seedlings have the advantages of regular growth, favorable characters and great quantity of root systems, can all expand into potatoes, can satisfy the artificial abundant culture demands, can grow quickly after being transplanted, and can implement large-area artificial culture of beautiful millettia roots and implement industrialization.

Description

technical field [0001] The invention relates to the cultivation technology of Chinese medicinal materials, in particular to a method for growing seedlings of Niu Dali, especially a method for raising seedlings by tissue culture of Niu Dali, and belongs to the technical field of cultivation of Chinese medicinal materials. Background technique [0002] Niu Dali is a plant of the genus Millettia specisoa of the leguminous family, scientific name: Millettia specisoa Champ, the root of Niu Dali is used as medicine, and its main ingredients are protein, starch and alkaloids. It belongs to the same origin of medicine and food, which can be used as medicine or food. The medicinal value of Niu Dali includes: nourishing the kidney and tonifying deficiency, strengthening tendons and activating collaterals, calming the liver and nourishing the lungs, treating kidney deficiency, and treating qi deficiency, backache, leg pain, rheumatism, chronic hepatitis, bronchitis, cough, tuberculosis...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 黄振忠
Owner 黄振忠
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