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Disposable cartridge for electroporation

A one-time, electroporation technology, applied in the direction of using electricity/wave energy to treat microorganisms, electrotherapy, and electricity/wave energy to treat enzymes, etc.

Pending Publication Date: 2017-05-24
ミルテニイビオテックベーファーウントコーカーゲー
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, it is not possible to automate the mixing of nucleic acid and cell suspension prior to the electroporation step

Method used

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  • Disposable cartridge for electroporation
  • Disposable cartridge for electroporation
  • Disposable cartridge for electroporation

Examples

Experimental program
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Embodiment Construction

[0032] The device of the present invention enables transfection of cells by mixing at least two fluids (the at least two fluids comprising cells and at least one compound to be electroporated into the cells) according to a predetermined volumetric mixing ratio and Apply an electric field to the mixed fluid.

[0033] The compound to be electroporated into the cell can be any compound known in the art to be useful for electroporation, such as nucleic acids, oligonucleotides, polynucleotides, DNA, RNA, peptides, proteins, and small molecules (such as hormones, cytokines, chemokines, drugs or prodrugs). In the following, the term "nucleic acid" as the compound to be electroporated is used for all such compounds to be electroporated (e.g. oligonucleotides, polynucleotides, DNA, RNA, peptides, proteins and small A synonym for a molecule such as a hormone, cytokine, chemokine, drug, or prodrug.

[0034]In order to perform electroporation on a homogeneously mixed fluid and prevent l...

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Abstract

The invention relates to a disposable cartridge for electroporation, and is directed to disposable for electroporation of cells, comprising a fluid compartment in an interior of the disposable; a first fluid port for providing cell suspension to the fluid compartment, and a second fluid port for delivering a fluid comprising at least one compound to be electroporated into the cells to the fluid compartment; a first electrode and a second electrode disposed in the fluid compartment; at least one exit port which delivers the fluid from the fluid compartment wherein the first and second fluid port have a fluid communication to a mixing channel which has a fluid communication to the fluid compartment.

Description

technical field [0001] The present invention relates to a disposable device for performing electroporation of cells, a process for electroporating cells using a disposable and a closed system comprising the disposable. Background technique [0002] Electroporation or electropermeabilization is a molecular biology technique in which an electric field is applied to cells in order to increase the permeability of the cell membrane, thereby allowing the introduction of nucleic acids into the cells. [0003] Electroporation is used to introduce nucleic acids into cells, especially mammalian cells. The transferred nucleic acid can be used for transient protein expression. In particular mRNA electroporation is used to transiently express proteins of interest. Plasmids can be used for transient expression as well as for stable integration. Integration of the plasmid into the genome can be improved by introducing double-strand breaks and homologous sequences that will replace the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12N13/00
CPCC12M23/16C12M35/02C12N13/00C12N15/87C12M23/28A61N1/327
Inventor E.卡巴哈J.博登贝格S.美天施R-P.彼得斯
Owner ミルテニイビオテックベーファーウントコーカーゲー
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