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Method for increasing content of GABA (gamma-aminobutyric acid) in brown rice

A technology of rice and GABA content, which is applied in the field of agriculture, can solve problems such as incomplete disclosure, achieve convenient operation, increase GABA content, and have obvious effects

Active Publication Date: 2017-05-24
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The existing technology has not fully revealed the mechanism of GABA accumulation in brown rice, is it also decarboxylated glutamic acid by glutamic acid decarboxylase? Whether the molecular mechanism for the further increase of GABA content in normal embryonic brown rice and giant embryonic brown rice during water immersion is due to Does glutamic acid synthesize GABA in large quantities under the action of glutamic acid decarboxylase?

Method used

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  • Method for increasing content of GABA (gamma-aminobutyric acid) in brown rice
  • Method for increasing content of GABA (gamma-aminobutyric acid) in brown rice
  • Method for increasing content of GABA (gamma-aminobutyric acid) in brown rice

Examples

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Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Molecular Mechanism of GABA Content Accumulation During Rice Seed Development

[0030] Arginine (Arg) can synthesize putrescine by arginine decarboxylase (ADC). Ornithine can be decarboxylated by ornithine decarboxylase (ODC) to synthesize putrescine. Polyamine oxidase (PAO) catalyzes the reverse synthesis of spermidine and spermine to form putrescine. Diamine oxidase (DAO) is the key enzyme for the production of GABA from putrescine.

[0031] Arginine decarboxylase (ADC), ornithine decarboxylase (ODC), polyamine oxidase (PAO) and diamine oxidase (DAO) belong to some important enzyme proteins in polyamine metabolic pathway respectively. Each of these enzyme proteins also has some isozyme members.

[0032] The normal-embryo rice "Chao 2-10" and the giant-embryo rice "Shangshida No. 5" were used as test materials and cultivated according to conventional methods. At 6 days, 10 days, 14 days, 18 days, 22 days, 26 days and 30 days after rice flowering, the cary...

Embodiment 2

[0052] Example 2 Changes in the content of polyamines caused by environmental changes enhance the accumulation of GABA content in brown rice

[0053] Yang et al. (Yang et al., J. Exp. Bot., 2007, 58(6):1545-1555) found that polyamine content in rice leaves would accumulate under drought conditions. There have been many reports that polyamines mostly accumulate under adversity. Polyamines are also associated with plant responses to environmental stress, including mineral nutrient deficiency, osmotic stress and drought, salt tolerance, low temperature, hypoxia and environmental pollution, etc. (Groppa et al., Amino Acids., 2008, 34:35-45; Alcazar et al., Planta, 2010, 231:1237-1249; Gill et al., Plant Signal Behav., 2010, 5:26-33).

[0054] This example is to explore two kinds of rice planted in different years. When the GABA content of brown rice is different, the factor affecting the GABA content in brown rice is the effect of the currently recognized glutamate pathway, or is...

Embodiment 3

[0060] Example 3 Brown rice immersion treatment increases GABA content

[0061] The normal embryo rice "Chao 2-10" and the giant embryo rice "Shangshida No. 5" were used as test materials. Soak brown rice in clear water at 25°C for 48 hours, change the clear water every 12 hours, drain the water after 48 hours and carry out germination at 28°C for 12 hours. Part of the germinated brown rice was frozen at -80°C for gene expression analysis of key enzymes in the GABA metabolic pathway, part of it was dried in the sun, then dried in an oven at 50°C, and stored in a refrigerator at 4°C for use with amino acid analyzers and The amino acid content, GABA content and three polyamine contents were analyzed by HPLC.

[0062] The expression level of glutamic acid decarboxylase gene OsGAD2 in the two kinds of rice tested was very low, but the other two glutamic acid decarboxylase genes OsGAD1 and OsGAD3 were expressed in the germinated brown rice of the two kinds of rice, but their expre...

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Abstract

The invention relates to the technical field of agriculture, and discloses a method for increasing content of GABA (gamma-aminobutyric acid) in brown rice. The method comprises the following steps of increasing the content of polyamine substance of related metabolites in a rice polyamine metabolic pathway, so as to reach the purpose of increasing the content of GABA in the brown rice; enhancing the expression of related genes in the polyamine metabolic pathway in the brown rice seeds brown rice, such as arginine decarboxylase genes, ornithine decarboxylase genes, polyamine oxidase genes or diamine oxidase genes; or performing adverse treatment on the rice, and inducing to increase the content of related polyamine metabolites in the brown rice. The method has the advantages that the content of GABA in the brown rice is greatly increased, the operation is convenient, and the effect is obvious; the method can be applied to common normal-embryo brown rice, and huge-embryo brown rice.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a new method for increasing the GABA content of brown rice. Background technique [0002] γ-aminobutyric acid, GABA for short, is a non-protein functional amino acid. GABA has very important health care functions for the human body. It has been reported that oral administration of rice germ rich in GABA can improve insomnia, depression and autonomic dysfunction symptoms in women with menopausal syndrome (Okada et al., J.Jpn.Soc.Food Sci., 2000, 47(8 ):596-603). American scholars Leventhal et al. published a report in the world-renowned magazine "Science" in 2003, confirming that GABA can improve the function of the visual cortex in aging monkeys (Leventhal et al., Science, 2003, 300 (5620): 812-815). Further research by Hua et al. found that in the visual cortex of aged cats, the density and proportion of neurons containing GABA were significantly less than those of youn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00A01G7/06
CPCA01G7/06C12N15/8251
Inventor 李建粤赵国超谢米雪张涛
Owner SHANGHAI NORMAL UNIVERSITY
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