Virus culture method

A virus culture and culture method technology, applied in the direction of virus, antiviral agent, 3D culture, etc., to achieve the effect of easy laboratory operation, speed up the experiment process, and easy operation

Active Publication Date: 2017-05-31
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is how to cultivate viruses, especially viruses that cannot or are difficult to cultivate in traditional in vitro cell culture. Viroids are characterized by inability to culture in two-dimensional cells, or in two-dimensional cells that can be cultured but cannot or are not easily separated or have low separation efficiency

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  • Virus culture method
  • Virus culture method

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Experimental program
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Embodiment 1

[0055] Embodiment 1, the cultivation of virus

[0056] 1. Prepare cells:

[0057] Resuscitate frozen primary or passaged respiratory epithelial cells (human primary respiratory epithelial cells HAE and passaged human bronchial epithelial-like cells BEAS-2B), at 37°C, 5.0% (volume percentage) CO 2 Culture in an incubator, and then perform three-dimensional cell culture on primary human respiratory epithelial cells HAE and passaged human bronchial epithelial cells BEAS-2B according to the method in step 2 below.

[0058] 2. Three-dimensional cell culture:

[0059] The following plan 1, plan 2 and plan 3 were respectively adopted for three-dimensional cell culture:

[0060] Option 1: Three-dimensional cells are cultivated in Matrigel culture system. The culture process is as follows: figure 1 As shown, the specific steps are as follows:

[0061] A. Select the primary or passaged respiratory epithelial cells in good condition for ordinary two-dimensional culture, and use them ...

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Abstract

The invention discloses a virus culture method. The virus culture method disclosed in the invention comprises the following steps: culturing animal cells by a three-dimensional cell culture method to obtain three-dimensional cultured cells; inoculating the three-dimensional cultured cells with a virus to perform the culture of the virus, wherein the virus is hosted in an animal; the animal is the following a1) or a2): a1) a mammal and a2) a human; the virus is the following 1) or 2): 1) a virus which cannot be cultured in a two-dimensional cell; 2) a virus which can be cultured in the two-dimensional cells but has low culture efficiency; the virus is the following b1) or b2): b1) respiratory virus; b2) a baculovirus or a group C rhinovirus; the animal cells are mammalian cells. Experiments show that the virus culture method provided by the invention can successfully culture the virus which cannot be cultured in the two-dimensional cells or can be cultured but has low culture efficiency.

Description

technical field [0001] The invention relates to a virus culture method in the field of biotechnology. Background technique [0002] Virus infection is one of the important causes of high morbidity and mortality of respiratory diseases in children worldwide. Many viruses have the characteristics of strong infectivity, rapid transmission, short incubation period, acute onset, and unsustainable immunity after illness. Disease pandemic. However, the development of virus research is often closely related to virus culture. At present, the in vitro culture isolation rate (reproduction efficiency) of some emerging respiratory viruses (such as human coronavirus and human metapneumovirus, etc.) is very low, and some emerging respiratory viruses (such as human bocavirus and group C rhinovirus, etc.) ) lacks an in vitro cell culture model. This has greatly hampered research efforts on emerging respiratory viruses. Therefore, it is urgent to establish a new generation of cell technol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N5/071A61K39/23A61K39/125A61P31/14A61P31/20
CPCA61K39/12C12N5/0625C12N7/00C12N2513/00C12N2750/14051C12N2750/14134C12N2770/32334C12N2770/32351
Inventor 胡步荣陈亚雄段招军潘冬杜亚蓉谢广成庞立丽宋敬东
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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