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60results about How to "Speed ​​up the experiment process" patented technology

High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) system and method

The invention discloses a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) system and method. The system comprises a high performance liquid chromatograph, a mass spectrometer, a pulse sampler, an electronic pulse device and an automatic data processing module. The method comprises the following steps: (1) generating an in vitro biological sample after a sample to be tested is subjected to an in vitro organism incubation experiment; (2) adding a stop solvent to stop a reaction; and (3) centrifuging the stopped biological sample, taking the supernatant and carrying out sample injection after dilution to carry out HPLC-MS / MS. By adopting the method, the analysis time can be greatly saved, the utilization rate of the instruments can be improved and the labor cost and other costs can be saved, thus saving lots of time in the new drug screening stage and speeding up the experiment process.
Owner:WUXI BIOLOGICS CO LTD +1

Automatic liquid volume quantifying and concentrating method with adjustable precision

The invention relates to an automatic liquid volume quantifying and concentrating method with adjustable precision, belonging to the field of testing. The functions of rapid concentrating with multiple choices and automatic volume quantifying with high precision are realized through the following assemblies: a basic assembly, a temperature control assembly, an air blow assembly, a negative pressure extraction and condensing assembly, a rotational shaking evaporation assembly and an automatic liquid level detection assembly which are arranged on a basic component. With the automatic liquid volume quantifying and concentrating method, the concentrating ways including heating, rotational evaporating, negative pressure extracting, air blowing and the like can be simultaneously provided for a user for selecting, so that the selectivity is wide, the flexibility is high, meanwhile, the experiment process is accelerated, and time and cost are saved; in addition, through the position match of two groups of sensors with low precision, the purpose of improving precision detection is improved, and the manufacturing cost is reduced. The method can be widely applied to the field of high-precision liquid level detection during the quantifying concentration process of chemical sample pretreatment.
Owner:PREEKEM SCI INSTR

Graphene sensitivity enhancing ITO metamaterial U-shaped SPR (Surface Plasmon Resonance) sensor

The invention relates to a graphene sensitivity enhancing ITO metamaterial U-shaped SPR (Surface Plasmon Resonance) sensor, which comprises a U-shaped optical fiber, wherein the U-shaped area of the U-shaped optical fiber is provided with a Gr / Au@ITO nanorod hybrid material structure. The preparation method comprises the steps of (1) preparing a U-shaped optical fiber, and preparing a gold nanoparticle layer in the U-shaped area of the U-shaped optical fiber; (2) preparing an ITO nano array structure; and (3) loading a graphene layer. According to the invention, the U-shaped optical fiber is adopted to serve as a transmission medium of light, the Gr / Au@ITO nanorod hybrid material structure is prepared on the surface of the U-shaped optical fiber, the sensitivity of the SPR sensor is enhanced by the highly enhanced local electromagnetic field generated in nanometer gaps between the uniform nanorod structures, and the sensitivity is further enhanced by the carrier flow brought by the graphene layer. The sensor has the outstanding advantages of small volume, light weight, easy carrying, high sensitivity, stable property, strong and short biological affinity and the like, and has wideapplication prospect in the detection aspects of trace gases, liquid, chemical elements, DNA and the like in the fields of biology, chemical engineering, medicine and the like.
Owner:SHANDONG NORMAL UNIV

Method for designing experimental scheme of pipe-conveying process evaluation based on complete relation active network

The invention relates to a method for designing an experimental scheme of pipe-conveying process evaluation based on a complete relation active network. The design method comprises the steps of: putting forward the complete relation active network based on the active network (AOV (Activity On Vertex) network), modeling and describing the experimental scheme by using the complete relation active network, examining logic of the scheme based on a relation rule library, and analyzing conflicts of the scheme by traversing the active network. The method can be used for describing complete equipment motion relation and control parameters conveniently during complex experiment process, so that the scheme is convenient to parse and execute and understood by process experimental staff. The scheme is designed in a visible editing manner and can generate an open scheme and a screening scheme required by a simulation experiment of pipe-conveying process evaluation. The design method disclosed by the invention provides the advanced and efficient scheme design method for the simulation experiment of crude oil pipe-conveying process evaluation and improves the automaticity of the pipe-conveying process evaluation experiment.
Owner:NANKAI UNIV

Constructing method and application for osteoportic fracture disease animal model

The invention provides a constructing method and application for an osteoportic fracture disease animal model. According to the provided technical scheme, based on a constructed osteoporosis animal model, thighbone small lateral incision fracture molding of a model animal is conducted, then a femur fractured end bilateral inverse intramedullary nail inserting internal fixation is conducted, and the osteoportic fracture disease animal model is constructed. The method avoids influences of uncontrollable factors on an experimental result, ensures the uniformity of modeling, shortens the molding time, and accelerates the experiment process. The method is applied to the preparation of the osteoportic fracture disease animal model and is beneficial for meeting related requirements of the osteoportic fracture disease animal model, and the osteoportic fracture disease animal model is taken as a study object to study the pathogenesis, prevention and treatment of basic and clinical osteoportic fractures.
Owner:FIRST PEOPLES HOSPITAL OF YUNNAN PROVINCE

On-orbit rapid calibration method for synthetic aperture radar based on natural uniform target

The invention relates to an on-orbit rapid calibration method for a synthetic aperture radar based on a natural uniform target. The method comprises the steps of carrying out cell division on an uncorrected image of a synthetic aperture radar needing to be calibrated; removing non-uniform area cells from the uncorrected image; averaging all azimuth strip data of the uncorrected image, fitting a distance strip, solving an antenna gain G, and performing relative correction on the uncorrected image to obtain a relative corrected image; taking the corrected image as a reference image, and registering the two images based on the feature points of the reference image and the uncorrected image; and calculating an absolute calibration parameter solution according to the processed reference image and the relative correction image, and solving an absolute calibration coefficient and an offset coefficient. According to the method, the on-orbit synthetic aperture radar is quickly calibrated basedon the distributed uniform targets in the calibrated synthetic aperture radar reference image and the relative correction image, so that the process of placing artificial reference equipment is omitted, the consumed manpower and material resources are reduced, and the calibration experiment process is accelerated.
Owner:ACAD OF OPTO ELECTRONICS CHINESE ACAD OF SCI +1

Method for preparing needle point of atomic force microscope

The invention relates to the field of atomic force microscopes, and provides a method for preparing a needle point of the atomic force microscope. A current feedback loop is composed of a power supply U, a resistor, a current feedback system, a cantilever, a needle point, a gap between the needle point and a sample, and the sample; concave pits with the diameter of 100nm and the depth of 30nm are formed in the surface of a first specific sample every 100nm; bulges with the height of 20nm and the diameter of 10nm are formed on the surface of a second specific sample every 20nm; a needle-shaped object is generated by the first specific sample, and a needle-shaped object, which is vertical to the surface of the sample, grows by utilizing the second specific sample; a parameter for determining whether the needle point is sharp or not is checked: the other sample with a relatively flat surface is used and works at a phase shaft mode; an initial amplitude is set to be AO, a scanning distance is set to be 15nm and a pre-set value is 0.6AO; if the phase shift on the flat surface is negative, the needle point is a sharp needle point capable of meeting experiment requirements; if the phase shift is not negative, a process of applying the power supply to form a high electric field in the gap between the needle point and the sample is repeated.
Owner:JINHUA VOCATIONAL TECH COLLEGE

Middle school physical experiment demonstration device and learning method thereof

The invention relates to a middle school physical experiment demonstration device and a learning method thereof and relates to an experiment device. An opening is arranged on an outer side wall of a cylinder, multiple plugging boards in interference fit with two inner side walls of the opening are arranged in the opening on the outer side wall of the cylinder, outer sides of the multiple pluggingboards are detachably provided with reducing type leakage nozzles, an outer surface of the cylinder is matched with annular boards matched with the cylinder in a gap matching mode, the annular boardsare provided with liquid collection boxes, the cylinder is clamped on a support, an upper portion of the support is hung with a spring dynamometer, a lower portion of the spring dynamometer is provided with a tightening sleeve rope, and an inner bottom surface of the cylinder is provided with a bottom fixed pulley. The experiment equipment is advantaged in that the experiment device not only can carry out demonstration teaching of the physical buoyancy experiment, but also can carry out targeted experimental demonstration on the buoyancy of the object at different depths in the solution, greatly deepens understanding and mastery of the physical buoyancy of students and greatly improves quality of teaching of teachers.
Owner:张策

Ultrasonic/microwave water heating/meto-super-critical reaction kettle

The invention discloses an ultrasonic / microwave water heating / meto-super-critical reaction kettle which comprises a kettle body, a kettle cover, an ultrasonic generator, a microwave generator, a controller, a microwave heating waveguide tube and a vibrator, wherein the kettle cover is positioned at the top end of the kettle body; the opening of the kettle body is sealed; a first connector for connecting the vibrator is arranged on the kettle body; at least one vibrator is provided and is connected with the ultrasonic generator; a second connector is arranged on the side wall of the bottom of the kettle body; the microwave heating waveguide tube is arranged at the second connector; the microwave heating waveguide tube is connected with the microwave generator; the controller is connected with the ultrasonic generator and the microwave generator respectively and used for controlling the working states of the ultrasonic generator and the microwave generator; a heat-preservation system andan electric heating system are arranged outside the kettle body; the electric heating system is connected with the controller; and the controller is used for controlling the working states of the electric heating system. By adopting the reaction kettle, the combination of multiple single experiment environments or multiple experiment environments can be achieved inside one reaction kettle, and diverse working modes are achieved.
Owner:SOUTHWEAT UNIV OF SCI & TECH

Cement mortar fluidity tester

The invention relates to a cement mortar fluidity tester. The cement mortar fluidity tester comprises a jumping table and a cleaning device, wherein the jumping table comprises a machine frame base (1), a cam (3), a first motor (4), a sleeve (5), a push rod (6), a fixed table top (9) and a jumping table top (12). The machine frame base (1) is composed of three steel vertical ribbed plates (2) which are fixed to a circular steel plate and fixedly connected with the sleeve (5) at 120 degrees. The push rod (6) is a cylindrical rod in the sleeve. The cement mortar fluidity tester has the beneficial effects that the table top can be cleaned, the diameter of the cement mortar can be measured, and meanwhile, the best experimental value can be selected by comparison; and meanwhile, the cement mortar can be put back into a net paste mixing pot, time is saved for the whole experimental process, the experimental process is speeded up, and manpower is further saved at a certain extent.
Owner:SHANXI UNIV

Radial seepage instrument and operation method thereof

The invention, which belongs to the field of geotechnical experiments, discloses a radial seepage instrument and an operation method thereof, thereby realizing the radial seepage experiment from the center to the periphery as well as the radial seepage experiment from the periphery to the center and realizing conversion between the two kinds of radial seepage experiments. The radial seepage instrument mainly includes a lower cover, an upper cover, an outer sleeve, a water-permeable cylinder and a water-permeable rod. A soil sample is in a circular ring shape. The water-permeable rod is inserted into the hole and is connected with the upper cover and the lower cover. Two main water paths flowing through the center hole and the periphery of the soil sample respectively are arranged in the radial seepage instrument to control water entrance and exit of the water paths; and thus water on one water path penetrates into the other water path along different radial directions of the soil sample to be discharged to a water volume measuring container. Therefore, internal gas can be exhausted as soon as possible conveniently at the early stage of the experiment; an effect of integration of one camera with two purposes is realized; and the structure is simple and resources and time are saved.
Owner:ZHENGZHOU UNIV

Method for fast detecting low-frequency exogenous chromosome fragments of genes with SSR markers

The invention discloses a method for detecting whether samples to be detected include target DNA. The method comprises the following steps: performing matrix arrangement to the samples to be detected; respectively mixing by rows and columns; respectively detecting whether the mixed samples include the target DNA or equivalent genetic markers thereof; marking the samples to be detected before mixing, corresponding to the mixed samples with positive results; if there is only one row mixed sample with the positive result and / or there is only one column mixed sample with the positive result, marking that the samples to be detected include the target DAN at two times and the rest of samples to be detected do not include the target DNA; if there are more than two row mixed samples with the positive result and column mixed samples with the positive result, respectively detecting whether the samples to be detected and marked at two times include the target DNA and the equivalent genetic markers thereof, wherein the samples to be detected with the positive result include the target DNA, and the rest of the samples to be detected do not include the target DNA. The method is used for tracking and identifying exogenous chromosome fragments of genes of plant genetic background so as to save the experiment cost, improve the working efficiency and quicken the experiment progress.
Owner:XINJIANG ACADEMY OF AGRI & RECLAMATION SCI

A flat pet system and its application

A flat-panel PET system for in vitro cell radionuclide experiments, including a data acquisition module, which includes two sets of parallel and oppositely placed PET detection flat panels to detect gamma photons emitted by cell samples; a data processing module to obtain PET detection flat panels The energy information, position information and time information of the received gamma photons are processed to obtain the projection data; the image reconstruction module: process the projection data to obtain the reconstructed image; and the cell culture plate fixing module is used to set the cell culture plate between the two PET detectors Between the plates, the mechanical control module is used to adjust the relative positions of the cell culture plate and the two groups of PET detection plates so that the cell culture plate is located at the center of the field of view of the flat PET system. It further improves the sensitivity of PET, can detect samples with lower activity, and is more suitable for the conditions of in vitro cell experiments. It can also obtain more data, obtain a higher image signal-to-noise ratio, and improve the accuracy of quantification.
Owner:湖北锐影科技有限公司

Isolation clamping device special for thin film thermocouple calibration

The invention discloses an isolation clamping device for thin film thermocouple calibration. The isolation clamping device comprises: a furnace tube cover fixed to an upper end of a temperature measuring furnace tube; a bracket having one end fixed on a surface of the furnace tube cover and the other end being fixedly connected to a guide barrel; the guide barrel whose center is fixedly aligned with a center position of the surface of the furnace tube cover; a sliding rod extending from the furnace tube cover and having an upper end being placed in the guide barrel, wherein a position of a thin film thermocouple to be tested in the temperature measuring furnace tube can be adjusted by changing the length of the sliding rod inserted into the temperature measuring furnace tube; and a clamping structure which is fixed on a lower end of the sliding rod and is capable of adjusting the strength of clamping the thin film thermocouple to be tested. The clamping structure is fixed on the lowerend of the sliding rod and can adjust the position of a sliding clamping sheet so as to clamp the thin film thermocouples to be tested of different sizes. The invention has the advantages of realizingseparation of a cold end from a hot end of the thin film thermocouple, reducing the adverse effect of an external environment on the stability of a temperature field in a temperature measuring furnace, and avoiding falling of a compensation wire.
Owner:DALIAN JIAOTONG UNIVERSITY

Virus culture method

The invention discloses a virus culture method. The virus culture method disclosed in the invention comprises the following steps: culturing animal cells by a three-dimensional cell culture method to obtain three-dimensional cultured cells; inoculating the three-dimensional cultured cells with a virus to perform the culture of the virus, wherein the virus is hosted in an animal; the animal is the following a1) or a2): a1) a mammal and a2) a human; the virus is the following 1) or 2): 1) a virus which cannot be cultured in a two-dimensional cell; 2) a virus which can be cultured in the two-dimensional cells but has low culture efficiency; the virus is the following b1) or b2): b1) respiratory virus; b2) a baculovirus or a group C rhinovirus; the animal cells are mammalian cells. Experiments show that the virus culture method provided by the invention can successfully culture the virus which cannot be cultured in the two-dimensional cells or can be cultured but has low culture efficiency.
Owner:INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI +1

Equivalent verification test method for reliability of thermal protection coating under high-temperature, high-pressure and high-frequency working conditions

The invention discloses an equivalent verification test method for reliability of a thermal protection coating under high-temperature, high-pressure and high-frequency working conditions, which comprises the following steps of: placing an equivalent test sample on an equivalent test bed, positively loading pressure stress on the coating under the high-temperature, high-pressure and high-frequency working conditions, performing a reliability test, and realizing verification when the coating is broken, wherein the equivalent test sample comprises a base body and a thermal protection coating arranged on the base body, the equivalent test bench comprises an upper clamp and a lower clamp, a guide rail is arranged on the lower clamp, a sliding block is arranged on the guide rail, a roller for placing a sample is vertically arranged on the sliding block, an upper clamp is arranged above the roller, and a pressure head for applying a load to the sample is arranged at the bottom end of the upper clamp. According to the method, the size of an equivalent bending sample is calculated from a non-equilibrium heat conduction equation and a bending stress formula, and then a bending fatigue test is carried out. The method is clear in principle, simple in test, good in equivalence, greatly shortened in period and low in test condition requirement.
Owner:XI AN JIAOTONG UNIV

Composite pile foundation of open caisson pile adding

The invention relates to a composite pile foundation of open caisson pile adding. The composite pile foundation comprises a single open caisson foundation, steel rod parts with threads, a side wall steel plate with a ring pull and counterweight pressing blocks, the centre of the lower part of the single open caisson foundation is provided with a plurality of steel rod parts with the threads, the upper ends of the steel rod parts with the threads areprovided with the counterweight pressing blocks, the counterweight pressing blocks are fixedly arranged at the bottom of the singleopen caisson foundation, and the outer side wall of thesingleopen caisson foundation is provided with the side wall steel plate with the ring pull. According to the composite pile foundation of the open caisson pileadding, the composite pile foundation is a novel open caisson pile adding composite foundation form with the adjustable length of a pile body, an adjustable stress position of a caisson body and the adjustable self-weight of the caisson body, and further a open caisson pile adding composite foundation form with an adjustableposition of a stress action point of the open caisson foundation, and thevariable length of the pile body and self-weight of the open caisson foundation in an experiment,indoor experiment operation of open caisson stress mechanism research is more convenient and fast, working efficiency is improved, and experimenting is more accurate.
Owner:NORTHEASTERN UNIV

Animal experiment device used for generating knee joint wear

The invention discloses an animal experiment device used for generating knee joint wear. The animal experiment device comprises a base body, the left part of the base body is provided with a high position base table, the high position base table is used for fixing a knee joint and an upper part arm of the knee joint of an experiment animal, the right part of the base body is provided with a low position base table, the low position base table is provided with a leg oscillation mechanism used for extruding and oscillating a shank of the experiment animal and an acting force adjustment mechanism used for automatically adjusting the amount of the stress of the knee joint, and the leg oscillation mechanism is fixedly installed on the action force adjustment mechanism. According to the animal experiment device used for generating the knee joint wear, animal knee joint motion wear inflammation needed by experiment personnel can be formed in a short time, the energy cost by the experiment personnel is relieved, the degree of the knee joint wear and the grade of the formed inflammation of the experiment animal can also be accurately controlled in the whole experiment forming process, and subsequent experiment operation is better benefited.
Owner:FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE

Spatial memory behavior training system guided by visual information

The invention provides a spatial memory behavior training system guided by visual information. The spatial memory behavior training system comprises a maze, a rotatable bracket fixedly connected with the maze, and an animal behavior analysis device which is used for main control and data acquisition and analysis and comprises a controller, an industrial camera, a first feeder, a second feeder and a third feeder, the maze comprises a square box body, two bosses, a starting arm, a first decision arm, a second decision arm, a first return arm, a second return arm and five lifting type automatic doors, wherein the square box body is composed of a bottom plate, a display and three frame plates, the upper end of the square box body is open, the two bosses are arranged in the square box body, the starting arm is composed of the two bosses and the square box body, and the lifting type automatic doors are arranged in the five arms respectively; and an experimental animal starting point area, a decision area and a reward area are arranged in the maze, and the starting arm, the first decision arm and the second decision arm present different visual prompts during an experiment. When the system is used, space information and visual information can be effectively separated in animal space guiding, interference of human factors is reduced to the maximum extent, and excessive extra interference information caused by back-and-forth movement of animals is reduced.
Owner:FUDAN UNIV

Identification method for cucumber-sour cucumber alien addition line material

The invention discloses an identification method for a cucumber-sour cucumber alien addition line material. The identification method comprises: carrying out backcross on a Cucumis allotriploid and diploid cultivation cucumber to obtain a cucumber-sour cucumber alien addition line candidate material, wherein a Cucumis amphidiploid new variety and diploid cucumber are subjected to reciprocal hybridization to obtain the Cucumis allotriploid; labeling a cucumber centromere specific repetitive sequence Type III by using a probe; taking the root tip of the cucumber-sour cucumber alien addition line candidate material, carrying out enzymolysis, and preparing a slice by using a flame drying method to obtain a metaphase chromosome slice having good dispersion; and denaturing the cucumber centromere specific repetitive sequence Type III probe, hybridizing the denatured probe onto the denatured metaphase chromosome slice, and making the Type III produce the clear and distinguishable fluorescent signal by using highly stringency hybridization conditions, such that the cucumber-sour cucumber alien addition line material and the number of the added sour cucumber chromosomes are identified. According to the present invention, the identification method creates the conditions for the transfer of the wild sour cucumber excellent gene in the alien addition line material and the cultivation of the new cucumber variety.
Owner:NANJING AGRICULTURAL UNIVERSITY

Virtual simulation experiment device capable of realizing multi-angle display

The invention discloses a virtual simulation experiment device capable of realizing multi-angle display. The device comprises an operation table positioned on a base, a display screen is arranged on the operation table, a plurality of rows of first sliding grooves are formed in the operation table in the horizontal direction of the operation table at intervals, a plurality of columns of second sliding grooves which penetrate through each row of first sliding grooves in the middle and are connected with each row of first sliding grooves in the two ends are formed in the operation table in the vertical direction of the operation table at intervals, and a clamping groove is formed in the intersection point of each first sliding groove and each second sliding groove. The experiment result canbe displayed on multiple screens, the experiment result can be displayed in a multi-angle and multi-dimensional three-dimensional mode according to the experiment requirement, the experiment result ismore three-dimensional, the experiment is operated by one person and the experiment result can be watched by multiple persons, the experiment efficiency is greatly improved, and the experiment process is accelerated.
Owner:QILU UNIV OF TECH

Primer for specifically amplifying RNA as well as design method and application of primer

The invention provides a reverse transcription primer. The reverse transcription primer is composed of an RNA template complementary part and an artificially introduced mismatched part. In a specific embodiment, the sequence of the reverse transcription primer is SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 10, and / or SEQ ID NO: 14. The invention also provides a method for designing the reverse transcription primer. By using the primer in RT-PCR, RNA can be specifically amplified, and DNA of the same sequence cannot be amplified.
Owner:NINGBO HEALTH GENE TECHNOLOGIES CO LTD

A kind of ethanol strain screening equipment

The invention discloses ethanol strain screening equipment. The ethanol strain screening equipment comprises an electromagnetic track fixedly arranged on a framework, wherein an electromagnetic slideblock is arranged on the lower end surface of the electromagnetic track in a sliding manner, a tank body A is fixedly arranged on the lower end surface of the electromagnetic slide block, a transmission cavity is formed in the tank body A, a transverse through cavity located in the tank body A is formed below the transmission cavity, a motor A is fixedly arranged in the upper end wall of the transmission cavity, a fixed plate is fixedly arranged on the lower end surface of an output shaft of the motor A, a rotating shaft which penetrates through the lower end wall of the transmission cavity and is in rotatable matching connection with the upper and lower end walls of the through cavity is fixedly arranged on the lower end surface of the fixed plate, a swing arm located in the through cavity is fixedly arranged on the outer surface of the rotating shaft, a fixed block is fixedly arranged on the right end surface of the swing arm, and a motor B is arranged in the right end surface of thefixed block. The ethanol strain screening equipment is simple in structure and convenient to use; and by utilizing the synergetic working of multiple motors, the automation of an ethanol strain screening process is realized, and the experimental efficiency is effectively improved.
Owner:五河县纬立农业科技有限公司

Efficient quantitative characterization method for complex-phase steel body-centered cubic structure phase

The invention provides an efficient quantitative characterization method for a complex-phase steel body-centered cubic structure phase, and belongs to the technical field of steel and iron material microscopic structure determination. The method comprises the following steps: firstly, preparing a metallographic sample, then carrying out constant-voltage electrolysis on the sample in an electrolyte, carrying out EBSD characterization on the electrolyzed sample, and selecting a step size which is 1 / 10 of the average grain size of the sample; the method comprises the following steps: carrying out EBSD original data processing by using OIM commercial software, independently exporting an Image Quality distribution table of a body-centered cubic structure phase, importing the obtained data into Excel for IQ numerical value standardization processing, importing the data into origin for Gauss multi-peak fitting, and finally obtaining the volume fractions of ferrite, bainite and martensite in a sample through the integral area proportion and position interval of each peak. According to the method, efficient and accurate quantitative characterization of the phase proportions of various body-centered cubic structures in the complex phase steel is achieved, and a new shortcut is provided for rapid and accurate quantitative analysis of multi-phase structures in research, development and analysis of complex phase steel materials.
Owner:UNIV OF SCI & TECH BEIJING

Bidirectional radial seepage instrument and using method thereof

The invention discloses a bidirectional radial seepage instrument and a using method thereof, and belongs to the field of geotechnical experiments. The bidirectional radial seepage instrument can be used for the radial seepage experiments from the center to the periphery and can also be used for the radial seepage experiments from the periphery to the center, and mainly comprises a combination ofa base, an outer sleeve and a water-permeable cylinder, an upper cover and a loading frame. The radial seepage instrument is internally provided with a lower gasket, a soil sample and an upper gasketin sequence from bottom to top. The soil sample takes the shape of a circular column and a water-permeable rod is inserted into the hole. The radial seepage instrument is internally provided with twomain water channels flowing through the center and the periphery of the soil sample to control the inflow and drainage of the water channels, so that the water of one water channel can penetrate intothe other water channel along different radial directions of the soil sample, so as to flow into a water quantity measurement container. The bidirectional radial seepage instrument is convenient to discharge the internal gas of the instrument as soon as possible in the initial stage of the experiment, few in component and dual-purpose, and is capable of saving the resources and time.
Owner:ZHENGZHOU UNIV

Fluorescence in situ hybridization method for high resolution chromosomes of cucumber

The invention provides a fluorescence in situ hybridization method, wherein fluorescence in situ hybridization with multiple probes is performed based on chromosomes in the meiosis pachytene stage of cucumber as a target DNA (Deoxyribonucleic Acid). The method comprises the following steps of: selecting high quality films with good chromosome dispersion and clear morphological structure, degenerating in 70% deionized formamide at 80 DEG C, and dehydrating and drying in gradient alcohol; extracting a target plasmid DNA by alkaline lysis, marking by an incision translation method, preparing a hybrid mixed liquor, degenerating at 90 DEG C; and loading to a glass slide to hybridize the target DNA and probe; and finally detecting signals. The fluorescence in situ hybridization technology provided by the invention can be applied to drawing a physical map of chromosomes of cucumber. As multiple probes can be added at one time, repeated hybridization to one film can be avoided, so that the experimental efficiency is greatly improved. According to the invention, appropriate whole genome DNA and Cot-1DNA of cucumber are added in the process of configuring the hybrid mixed liquor for blocking spurious signals and repetitive sequence signals, so that the identification rate of target signals is improved and the accuracy of experimental result is increased.
Owner:NANJING AGRICULTURAL UNIVERSITY

Humidity-heat aging test method for photovoltaic module

The invention discloses a damp-heat aging test method for a photovoltaic module, which comprises the following steps: preparing a sample: providing a bare battery piece, and welding a welding strip on the front surface of the bare battery piece to obtain the sample; the sample testing step comprises the steps that initial testing is carried out on the sample to obtain an initial testing result, and the initial testing comprises power testing, PL testing and EL testing; putting the sample subjected to the initial test into a damp-heat aging oven for testing, and setting the testing conditions of the damp-heat aging oven as follows: the testing temperature is 85 + / -1 DEG C, the relative humidity is 85 + / -1%, and the testing time is 24 hours; and a sample result analysis step: performing post-detection on the sample to obtain a post-test result, the post-detection comprising power test, PL test and EL test, and calculating an attenuation value according to the initial test result and the post-test result. The method is closer to the state of a bare battery piece in an actual photovoltaic module product, the corrosion speed of the sample is increased, the experiment process is accelerated, and a large number of test resources are saved.
Owner:ZHEJIANG JINKO SOLAR CO LTD +1

Circulating salt spray accelerated corrosion test device

The invention discloses a circulating salt spray accelerated corrosion test device, and relates to the technical field of salt spray test devices, the circulating salt spray accelerated corrosion test device comprises a box body, a support plate is arranged in the box body, a fixed plate is fixedly connected to the middle of the upper surface of the support plate, two hydraulic cylinders are fixedly connected to the upper surface of the support plate, and telescopic ends of the hydraulic cylinders are fixedly connected with a movable plate; limiting plates are fixedly connected to the front and rear sides of the movable plate, a rotating shaft is fixedly connected to the middle of the lower surface of the supporting plate, a motor is arranged in the middle of the lower surface of the box, and the bottom end of the rotating shaft penetrates the middle of the bottom end of the box and is fixedly connected with an output shaft of the motor. Under the cooperation of the hydraulic cylinder, the movable plate and the fixed plate, the signal processing plates are convenient to fix, so that a plurality of signal processing plates are convenient to test, and the problem that an existing salt spray corrosion test device can only test one signal processing plate and accordingly test inaccuracy is caused by contingency is solved.
Owner:CENT TESTING INT GRP CO LTD

High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) system and method

The invention discloses a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) system and method. The system comprises a high performance liquid chromatograph, a mass spectrometer, a pulse sampler, an electronic pulse device and an automatic data processing module. The method comprises the following steps: (1) generating an in vitro biological sample after a sample to be tested is subjected to an in vitro organism incubation experiment; (2) adding a stop solvent to stop a reaction; and (3) centrifuging the stopped biological sample, taking the supernatant and carrying out sample injection after dilution to carry out HPLC-MS / MS. By adopting the method, the analysis time can be greatly saved, the utilization rate of the instruments can be improved and the labor cost and other costs can be saved, thus saving lots of time in the new drug screening stage and speeding up the experiment process.
Owner:WUXI BIOLOGICS CO LTD +1
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