60results about How to "Speed ​​up the experiment process" patented technology

The invention relates to a graphene sensitivity enhancing ITO metamaterial U-shaped SPR (Surface Plasmon Resonance) sensor, which comprises a U-shaped optical fiber, wherein the U-shaped area of the U-shaped optical fiber is provided with a Gr/Au@ITO nanorod hybrid material structure. The preparation method comprises the steps of (1) preparing a U-shaped optical fiber, and preparing a gold nanoparticle layer in the U-shaped area of the U-shaped optical fiber; (2) preparing an ITO nano array structure; and (3) loading a graphene layer. According to the invention, the U-shaped optical fiber is adopted to serve as a transmission medium of light, the Gr/Au@ITO nanorod hybrid material structure is prepared on the surface of the U-shaped optical fiber, the sensitivity of the SPR sensor is enhanced by the highly enhanced local electromagnetic field generated in nanometer gaps between the uniform nanorod structures, and the sensitivity is further enhanced by the carrier flow brought by the graphene layer. The sensor has the outstanding advantages of small volume, light weight, easy carrying, high sensitivity, stable property, strong and short biological affinity and the like, and has wideapplication prospect in the detection aspects of trace gases, liquid, chemical elements, DNA and the like in the fields of biology, chemical engineering, medicine and the like.

The invention provides a constructing method and application for an osteoportic fracture disease animal model. According to the provided technical scheme, based on a constructed osteoporosis animal model, thighbone small lateral incision fracture molding of a model animal is conducted, then a femur fractured end bilateral inverse intramedullary nail inserting internal fixation is conducted, and the osteoportic fracture disease animal model is constructed. The method avoids influences of uncontrollable factors on an experimental result, ensures the uniformity of modeling, shortens the molding time, and accelerates the experiment process. The method is applied to the preparation of the osteoportic fracture disease animal model and is beneficial for meeting related requirements of the osteoportic fracture disease animal model, and the osteoportic fracture disease animal model is taken as a study object to study the pathogenesis, prevention and treatment of basic and clinical osteoportic fractures.

The invention relates to an on-orbit rapid calibration method for a synthetic aperture radar based on a natural uniform target. The method comprises the steps of carrying out cell division on an uncorrected image of a synthetic aperture radar needing to be calibrated; removing non-uniform area cells from the uncorrected image; averaging all azimuth strip data of the uncorrected image, fitting a distance strip, solving an antenna gain G, and performing relative correction on the uncorrected image to obtain a relative corrected image; taking the corrected image as a reference image, and registering the two images based on the feature points of the reference image and the uncorrected image; and calculating an absolute calibration parameter solution according to the processed reference image and the relative correction image, and solving an absolute calibration coefficient and an offset coefficient. According to the method, the on-orbit synthetic aperture radar is quickly calibrated basedon the distributed uniform targets in the calibrated synthetic aperture radar reference image and the relative correction image, so that the process of placing artificial reference equipment is omitted, the consumed manpower and material resources are reduced, and the calibration experiment process is accelerated.

The invention relates to a middle school physical experiment demonstration device and a learning method thereof and relates to an experiment device. An opening is arranged on an outer side wall of a cylinder, multiple plugging boards in interference fit with two inner side walls of the opening are arranged in the opening on the outer side wall of the cylinder, outer sides of the multiple pluggingboards are detachably provided with reducing type leakage nozzles, an outer surface of the cylinder is matched with annular boards matched with the cylinder in a gap matching mode, the annular boardsare provided with liquid collection boxes, the cylinder is clamped on a support, an upper portion of the support is hung with a spring dynamometer, a lower portion of the spring dynamometer is provided with a tightening sleeve rope, and an inner bottom surface of the cylinder is provided with a bottom fixed pulley. The experiment equipment is advantaged in that the experiment device not only can carry out demonstration teaching of the physical buoyancy experiment, but also can carry out targeted experimental demonstration on the buoyancy of the object at different depths in the solution, greatly deepens understanding and mastery of the physical buoyancy of students and greatly improves quality of teaching of teachers.

The invention relates to a cement mortar fluidity tester. The cement mortar fluidity tester comprises a jumping table and a cleaning device, wherein the jumping table comprises a machine frame base (1), a cam (3), a first motor (4), a sleeve (5), a push rod (6), a fixed table top (9) and a jumping table top (12). The machine frame base (1) is composed of three steel vertical ribbed plates (2) which are fixed to a circular steel plate and fixedly connected with the sleeve (5) at 120 degrees. The push rod (6) is a cylindrical rod in the sleeve. The cement mortar fluidity tester has the beneficial effects that the table top can be cleaned, the diameter of the cement mortar can be measured, and meanwhile, the best experimental value can be selected by comparison; and meanwhile, the cement mortar can be put back into a net paste mixing pot, time is saved for the whole experimental process, the experimental process is speeded up, and manpower is further saved at a certain extent.

The invention, which belongs to the field of geotechnical experiments, discloses a radial seepage instrument and an operation method thereof, thereby realizing the radial seepage experiment from the center to the periphery as well as the radial seepage experiment from the periphery to the center and realizing conversion between the two kinds of radial seepage experiments. The radial seepage instrument mainly includes a lower cover, an upper cover, an outer sleeve, a water-permeable cylinder and a water-permeable rod. A soil sample is in a circular ring shape. The water-permeable rod is inserted into the hole and is connected with the upper cover and the lower cover. Two main water paths flowing through the center hole and the periphery of the soil sample respectively are arranged in the radial seepage instrument to control water entrance and exit of the water paths; and thus water on one water path penetrates into the other water path along different radial directions of the soil sample to be discharged to a water volume measuring container. Therefore, internal gas can be exhausted as soon as possible conveniently at the early stage of the experiment; an effect of integration of one camera with two purposes is realized; and the structure is simple and resources and time are saved.

The invention discloses a method for detecting whether samples to be detected include target DNA. The method comprises the following steps: performing matrix arrangement to the samples to be detected; respectively mixing by rows and columns; respectively detecting whether the mixed samples include the target DNA or equivalent genetic markers thereof; marking the samples to be detected before mixing, corresponding to the mixed samples with positive results; if there is only one row mixed sample with the positive result and/or there is only one column mixed sample with the positive result, marking that the samples to be detected include the target DAN at two times and the rest of samples to be detected do not include the target DNA; if there are more than two row mixed samples with the positive result and column mixed samples with the positive result, respectively detecting whether the samples to be detected and marked at two times include the target DNA and the equivalent genetic markers thereof, wherein the samples to be detected with the positive result include the target DNA, and the rest of the samples to be detected do not include the target DNA. The method is used for tracking and identifying exogenous chromosome fragments of genes of plant genetic background so as to save the experiment cost, improve the working efficiency and quicken the experiment progress.

A flat-panel PET system for in vitro cell radionuclide experiments, including a data acquisition module, which includes two sets of parallel and oppositely placed PET detection flat panels to detect gamma photons emitted by cell samples; a data processing module to obtain PET detection flat panels The energy information, position information and time information of the received gamma photons are processed to obtain the projection data; the image reconstruction module: process the projection data to obtain the reconstructed image; and the cell culture plate fixing module is used to set the cell culture plate between the two PET detectors Between the plates, the mechanical control module is used to adjust the relative positions of the cell culture plate and the two groups of PET detection plates so that the cell culture plate is located at the center of the field of view of the flat PET system. It further improves the sensitivity of PET, can detect samples with lower activity, and is more suitable for the conditions of in vitro cell experiments. It can also obtain more data, obtain a higher image signal-to-noise ratio, and improve the accuracy of quantification.

The invention discloses a construction method and device for a depression-induced breast cancer animal model and application. The construction method comprises the following steps that a mouse is placed in a constraining device not to act, the mouse is constrained for 4 hours every day, light-dark circulation is maintained for 12 hours for 3 weeks or more, and constructing is carried out to obtain the spontaneous breast cancer transgenic mouse depressive disorder animal model with depressive disorder related phenotypes. The invention further discloses a constraint device of the animal model. The animal model is used for simulating the human breast cancer and depression process and screening diagnosis and treatment drugs or therapies. The animal model has the advantages of good repeatability, small individual difference, short modeling time, low animal death rate and the like, and can be widely applied to simulation of human depression-induced breast cancer and related disease pathology and screening and curative effect verification of diagnosis and treatment substances.

The invention relates to a composite pile foundation of open caisson pile adding. The composite pile foundation comprises a single open caisson foundation, steel rod parts with threads, a side wall steel plate with a ring pull and counterweight pressing blocks, the centre of the lower part of the single open caisson foundation is provided with a plurality of steel rod parts with the threads, the upper ends of the steel rod parts with the threads areprovided with the counterweight pressing blocks, the counterweight pressing blocks are fixedly arranged at the bottom of the singleopen caisson foundation, and the outer side wall of thesingleopen caisson foundation is provided with the side wall steel plate with the ring pull. According to the composite pile foundation of the open caisson pileadding, the composite pile foundation is a novel open caisson pile adding composite foundation form with the adjustable length of a pile body, an adjustable stress position of a caisson body and the adjustable self-weight of the caisson body, and further a open caisson pile adding composite foundation form with an adjustableposition of a stress action point of the open caisson foundation, and thevariable length of the pile body and self-weight of the open caisson foundation in an experiment,indoor experiment operation of open caisson stress mechanism research is more convenient and fast, working efficiency is improved, and experimenting is more accurate.

The invention discloses an animal experiment device used for generating knee joint wear. The animal experiment device comprises a base body, the left part of the base body is provided with a high position base table, the high position base table is used for fixing a knee joint and an upper part arm of the knee joint of an experiment animal, the right part of the base body is provided with a low position base table, the low position base table is provided with a leg oscillation mechanism used for extruding and oscillating a shank of the experiment animal and an acting force adjustment mechanism used for automatically adjusting the amount of the stress of the knee joint, and the leg oscillation mechanism is fixedly installed on the action force adjustment mechanism. According to the animal experiment device used for generating the knee joint wear, animal knee joint motion wear inflammation needed by experiment personnel can be formed in a short time, the energy cost by the experiment personnel is relieved, the degree of the knee joint wear and the grade of the formed inflammation of the experiment animal can also be accurately controlled in the whole experiment forming process, and subsequent experiment operation is better benefited.

The invention discloses an identification method for a cucumber-sour cucumber alien addition line material. The identification method comprises: carrying out backcross on a Cucumis allotriploid and diploid cultivation cucumber to obtain a cucumber-sour cucumber alien addition line candidate material, wherein a Cucumis amphidiploid new variety and diploid cucumber are subjected to reciprocal hybridization to obtain the Cucumis allotriploid; labeling a cucumber centromere specific repetitive sequence Type III by using a probe; taking the root tip of the cucumber-sour cucumber alien addition line candidate material, carrying out enzymolysis, and preparing a slice by using a flame drying method to obtain a metaphase chromosome slice having good dispersion; and denaturing the cucumber centromere specific repetitive sequence Type III probe, hybridizing the denatured probe onto the denatured metaphase chromosome slice, and making the Type III produce the clear and distinguishable fluorescent signal by using highly stringency hybridization conditions, such that the cucumber-sour cucumber alien addition line material and the number of the added sour cucumber chromosomes are identified. According to the present invention, the identification method creates the conditions for the transfer of the wild sour cucumber excellent gene in the alien addition line material and the cultivation of the new cucumber variety.

The invention provides a reverse transcription primer. The reverse transcription primer is composed of an RNA template complementary part and an artificially introduced mismatched part. In a specific embodiment, the sequence of the reverse transcription primer is SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 10, and/or SEQ ID NO: 14. The invention also provides a method for designing the reverse transcription primer. By using the primer in RT-PCR, RNA can be specifically amplified, and DNA of the same sequence cannot be amplified.

The invention provides an efficient quantitative characterization method for a complex-phase steel body-centered cubic structure phase, and belongs to the technical field of steel and iron material microscopic structure determination. The method comprises the following steps: firstly, preparing a metallographic sample, then carrying out constant-voltage electrolysis on the sample in an electrolyte, carrying out EBSD characterization on the electrolyzed sample, and selecting a step size which is 1/10 of the average grain size of the sample; the method comprises the following steps: carrying out EBSD original data processing by using OIM commercial software, independently exporting an Image Quality distribution table of a body-centered cubic structure phase, importing the obtained data into Excel for IQ numerical value standardization processing, importing the data into origin for Gauss multi-peak fitting, and finally obtaining the volume fractions of ferrite, bainite and martensite in a sample through the integral area proportion and position interval of each peak. According to the method, efficient and accurate quantitative characterization of the phase proportions of various body-centered cubic structures in the complex phase steel is achieved, and a new shortcut is provided for rapid and accurate quantitative analysis of multi-phase structures in research, development and analysis of complex phase steel materials.

The invention discloses a bidirectional radial seepage instrument and a using method thereof, and belongs to the field of geotechnical experiments. The bidirectional radial seepage instrument can be used for the radial seepage experiments from the center to the periphery and can also be used for the radial seepage experiments from the periphery to the center, and mainly comprises a combination ofa base, an outer sleeve and a water-permeable cylinder, an upper cover and a loading frame. The radial seepage instrument is internally provided with a lower gasket, a soil sample and an upper gasketin sequence from bottom to top. The soil sample takes the shape of a circular column and a water-permeable rod is inserted into the hole. The radial seepage instrument is internally provided with twomain water channels flowing through the center and the periphery of the soil sample to control the inflow and drainage of the water channels, so that the water of one water channel can penetrate intothe other water channel along different radial directions of the soil sample, so as to flow into a water quantity measurement container. The bidirectional radial seepage instrument is convenient to discharge the internal gas of the instrument as soon as possible in the initial stage of the experiment, few in component and dual-purpose, and is capable of saving the resources and time.

The invention provides a fluorescence in situ hybridization method, wherein fluorescence in situ hybridization with multiple probes is performed based on chromosomes in the meiosis pachytene stage of cucumber as a target DNA (Deoxyribonucleic Acid). The method comprises the following steps of: selecting high quality films with good chromosome dispersion and clear morphological structure, degenerating in 70% deionized formamide at 80 DEG C, and dehydrating and drying in gradient alcohol; extracting a target plasmid DNA by alkaline lysis, marking by an incision translation method, preparing a hybrid mixed liquor, degenerating at 90 DEG C; and loading to a glass slide to hybridize the target DNA and probe; and finally detecting signals. The fluorescence in situ hybridization technology provided by the invention can be applied to drawing a physical map of chromosomes of cucumber. As multiple probes can be added at one time, repeated hybridization to one film can be avoided, so that the experimental efficiency is greatly improved. According to the invention, appropriate whole genome DNA and Cot-1DNA of cucumber are added in the process of configuring the hybrid mixed liquor for blocking spurious signals and repetitive sequence signals, so that the identification rate of target signals is improved and the accuracy of experimental result is increased.

The invention discloses a damp-heat aging test method for a photovoltaic module, which comprises the following steps: preparing a sample: providing a bare battery piece, and welding a welding strip on the front surface of the bare battery piece to obtain the sample; the sample testing step comprises the steps that initial testing is carried out on the sample to obtain an initial testing result, and the initial testing comprises power testing, PL testing and EL testing; putting the sample subjected to the initial test into a damp-heat aging oven for testing, and setting the testing conditions of the damp-heat aging oven as follows: the testing temperature is 85 +/-1 DEG C, the relative humidity is 85 +/-1%, and the testing time is 24 hours; and a sample result analysis step: performing post-detection on the sample to obtain a post-test result, the post-detection comprising power test, PL test and EL test, and calculating an attenuation value according to the initial test result and the post-test result. The method is closer to the state of a bare battery piece in an actual photovoltaic module product, the corrosion speed of the sample is increased, the experiment process is accelerated, and a large number of test resources are saved.

The invention discloses a circulating salt spray accelerated corrosion test device, and relates to the technical field of salt spray test devices, the circulating salt spray accelerated corrosion test device comprises a box body, a support plate is arranged in the box body, a fixed plate is fixedly connected to the middle of the upper surface of the support plate, two hydraulic cylinders are fixedly connected to the upper surface of the support plate, and telescopic ends of the hydraulic cylinders are fixedly connected with a movable plate; limiting plates are fixedly connected to the front and rear sides of the movable plate, a rotating shaft is fixedly connected to the middle of the lower surface of the supporting plate, a motor is arranged in the middle of the lower surface of the box, and the bottom end of the rotating shaft penetrates the middle of the bottom end of the box and is fixedly connected with an output shaft of the motor. Under the cooperation of the hydraulic cylinder, the movable plate and the fixed plate, the signal processing plates are convenient to fix, so that a plurality of signal processing plates are convenient to test, and the problem that an existing salt spray corrosion test device can only test one signal processing plate and accordingly test inaccuracy is caused by contingency is solved.