High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) system and method
A high-performance liquid chromatography and high-performance liquid chromatography technology, which is applied in the field of high-performance liquid chromatography tandem mass spectrometry analysis system and its analysis method, can solve the disadvantage of less sample and make full use of the instrument, the automatic sampler has a long delay time, and the sample analysis time is prolonged. problems, to achieve the effect of improving the utilization rate of the instrument, saving manpower and high selectivity
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[0030] The high-performance liquid phase tandem mass spectrometry (HPLC-MS / MS) analysis system used for drug screening and biological sample analysis in this embodiment, its system connection diagram is as follows figure 1 shown, including:
[0031] Japan Shimadzu high performance liquid chromatograph LC 20 AD (Shimadzu Inc), in the present embodiment, the chromatographic column is the C18 reversed-phase chromatographic column of Synergi Hydro-RP (Phenomenex.Inc) that the chromatographic column is 3cm long model; Japan Shimadzu high performance liquid phase Chromatograph, the system is binary pump chromatography, which is characterized by good compatibility with mass spectrometry, easy operation and relatively fast liquid phase pump, and its main function is to quickly provide gradient liquid phase changes;
[0032] API 4000 mass spectrometer (Applied Biosystems Inc), this mass spectrometer equipment is an analyzer, and its scanning speed is fast, can match with the fast injec...
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