High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) system and method

Abstract

The invention discloses a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) system and method. The system comprises a high performance liquid chromatograph, a mass spectrometer, a pulse sampler, an electronic pulse device and an automatic data processing module. The method comprises the following steps: (1) generating an in vitro biological sample after a sample to be tested is subjected to an in vitro organism incubation experiment; (2) adding a stop solvent to stop a reaction; and (3) centrifuging the stopped biological sample, taking the supernatant and carrying out sample injection after dilution to carry out HPLC-MS / MS. By adopting the method, the analysis time can be greatly saved, the utilization rate of the instruments can be improved and the labor cost and other costs can be saved, thus saving lots of time in the new drug screening stage and speeding up the experiment process.

Description

technical field [0001] The invention relates to a liquid phase tandem mass spectrometry (LC-MS / MS) system and method, in particular to a high performance liquid phase tandem mass spectrometry system and an analysis method for drug screening and biological sample analysis. Background technique [0002] High throughput screening (HTS) technology is an indispensable part of the new drug development process. The long cycle of new drug development requires that the development efficiency must be improved. Therefore, high-throughput screening methods have been vigorously promoted. [0003] LC-MS / MS is a separation, analysis and detection instrument that is used in series with liquid chromatography and mass spectrometer. It mainly uses the excellent separation effect of liquid chromatography and the high sensitivity and high selectivity of mass spectrometry to complete the chemical analysis of compounds in the field of medicine. High-throughput screening and further research. [...

AI Technical Summary

Problems solved by technology

[0004] There are many kinds of LC-MS / MS coupling methods. At present, ordinary HPLC (High Performance Liquid Chromatography, high performance liquid chromatography) and CTC autosampler (CTC company in Switzerland) are used in series with mass spectrometry, and there are also self-contained ones. The HPLC or UPLC (Ultra Performance Liquid chromatography) of the autosampler is used in series with the mass spectrometer. The disadvantage of both is that the delay time of the autosampler is too long, which leads to the prolongation of the entire sample analysis time and waste. In addition, because the built-in autosampler has fewer plates, the number of samples that can be carried at one time is relatively small, which is not conducive to making full use of the instrument.

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