Method for fast detecting low-frequency exogenous chromosome fragments of genes with SSR markers

A technology of markers and genetic markers, applied in the direction of biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of long-term, large manpower and material resources, etc.

Inactive Publication Date: 2013-01-23
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Abstract
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  • Claims
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Problems solved by technology

If one by one screening and identification, not only need a lot of manpower and material resources, but also take a long time to obtain the identification results

Method used

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  • Method for fast detecting low-frequency exogenous chromosome fragments of genes with SSR markers
  • Method for fast detecting low-frequency exogenous chromosome fragments of genes with SSR markers
  • Method for fast detecting low-frequency exogenous chromosome fragments of genes with SSR markers

Examples

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Embodiment 1

[0049] Example 1. Using SSR markers to quickly detect small fragments or genes of low-frequency exogenous chromosomes

[0050] In this embodiment, the method for rapidly detecting small fragments or genes of low-frequency exogenous chromosomes using SSR markers specifically includes the following steps:

[0051] (a) Arrange the N samples to be tested according to the matrix A shown in formula (1);

[0052] A = a 11 a 12 · · · a 1 n a 21 a ...

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Abstract

The invention discloses a method for detecting whether samples to be detected include target DNA. The method comprises the following steps: performing matrix arrangement to the samples to be detected; respectively mixing by rows and columns; respectively detecting whether the mixed samples include the target DNA or equivalent genetic markers thereof; marking the samples to be detected before mixing, corresponding to the mixed samples with positive results; if there is only one row mixed sample with the positive result and / or there is only one column mixed sample with the positive result, marking that the samples to be detected include the target DAN at two times and the rest of samples to be detected do not include the target DNA; if there are more than two row mixed samples with the positive result and column mixed samples with the positive result, respectively detecting whether the samples to be detected and marked at two times include the target DNA and the equivalent genetic markers thereof, wherein the samples to be detected with the positive result include the target DNA, and the rest of the samples to be detected do not include the target DNA. The method is used for tracking and identifying exogenous chromosome fragments of genes of plant genetic background so as to save the experiment cost, improve the working efficiency and quicken the experiment progress.

Description

technical field [0001] The invention relates to a method for detecting whether a sample to be tested contains target DNA, in particular to a method for rapidly detecting small fragments or genes of low-frequency exogenous chromosomes by using SSR markers. Background technique [0002] Plant genetic improvement is the process of achieving good gene aggregation through the selection of target genes. If the transferred gene and its carrier—exogenous chromosome or chromosome fragment are tracked and identified during the process of introducing the target gene into the plant, the accuracy of selection can be improved, thereby shortening the breeding cycle and improving breeding efficiency. Therefore, it is very important to effectively identify the exogenous chromosomes or chromosome fragments introduced into the plant background and the exogenous genes carried by them. At present, the methods for identifying foreign chromosomes or genes based on genetic marker systems mainly in...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 宿俊吉陈红相吉山邓福军吴嫚甘尚权刘洋
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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