Tuna bone collagen peptide capable of promoting bone cell growth and preparation method thereof

A technology of tuna and collagen, applied in the field of biomedicine, can solve the problems of insufficient utilization, waste of resources, etc., and achieve the effects of improving production efficiency, reducing energy consumption, and having no toxic and side effects

Active Publication Date: 2017-05-31
BEIJING UNIV OF CHEM TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the utilization of leftovers from aquatic product processing is not sufficient, resulting in waste of resources

Method used

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  • Tuna bone collagen peptide capable of promoting bone cell growth and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Taking tuna fish bone as raw material to prepare and promote osteoblast growth as an example, the following process steps are carried out in sequence:

[0029] (1) Decalcification treatment: thaw the tuna fish bones, wash them with cold water, soak the fish bones in 0.4wt% hydrochloric acid solution at room temperature for 7.5 hours, replace the acid solution every 1.5 hours, and remove calcium salts; % sodium chloride to soak fish bones for 12 hours to remove miscellaneous proteins (miscellaneous proteins are non-collagen proteins);

[0030] (2) Compound enzyme fish bone degreasing enzymatic hydrolysis: clean the fish bone, drain, add deionized water at a weight ratio of fish bone to deionized water of 1:10, adjust the pH to 7, and add it to medium according to the concentration of 0.4wt% lipase (triacylglycerol acyl hydrolase), a collagenase was added at a concentration of 0.5wt%, papain was added at a concentration of 0.5wt%, the enzymolysis temperature was adjusted ...

Embodiment 2

[0035] Taking tuna fish bone as raw material to prepare and promote osteoblast growth as an example, the following process steps are carried out in sequence:

[0036] (1) Decalcification treatment: thaw the tuna fish bones, wash them with cold water, soak the fish bones in 0.5wt% hydrochloric acid solution at room temperature for 6 hours, replace the acid solution every 1.5 hours, and remove calcium salts; Soak the fish bones with 100% sodium chloride for 10 hours to remove miscellaneous proteins (miscellaneous proteins are non-collagen proteins);

[0037] (2) Compound enzyme fish bone degreasing enzymatic hydrolysis: clean the fish bone, drain, add deionized water at a weight ratio of fish bone to deionized water of 1:10, adjust the pH to 9, and add alkali according to the concentration of 0.4wt% Sexual lipase (triacylglycerol acyl hydrolase), add alkaline protease (Novo type serine protease) according to the concentration of 0.5wt%, adjust the enzymolysis temperature to 50°C...

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Abstract

The invention relates to tuna bone collagen peptide capable of promoting bone cell growth and a preparation method thereof. The method comprises the steps of decalcification treatment, compound enzyme fish bone degreasing enzymolysis and purification and drying; for the tuna bone collagen peptide obtained through the method, the molecular weight is 500-2,000 Da, the contact angle is 10.0-25.0 degrees, and MC3T3-E1 cell growth can be promoted under the concentration of 5-200 microgram/mL. Accordingly, the preparation technology is scientific and reasonable, fish bones which are not utilized in aquatic product processing are fully utilized as raw materials, collagen peptide with potential market value is developed, compound enzyme degreasing enzymolysis method is adopted, the compound enzyme enzymolysis condition is adjusted, and the tuna bone collagen peptide which is capable of promoting the bone cell growth, safe, free of toxic and side effects and the like is obtained. The production efficiency is improved, energy consumption is lowered, the technology is scientific and reasonable, and operation is easy.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a fish bone collagen polypeptide and a preparation method thereof, in particular to a tuna fish bone collagen polypeptide for promoting bone cell growth and a preparation method thereof. Background technique [0002] Osteoporosis is a common human disease, because it seriously endangers human health, so people pay more and more attention to the treatment of osteoporosis. Bone is composed of organic / inorganic composite materials, namely collagen and hydroxyapatite, so the loss of collagen will inevitably affect the calcium content in bone. Therefore, increasing the content of collagen in bone is a reliable method for treating osteoporosis. Collagen polypeptide is a hydrolyzate obtained by hydrolyzing collagen under certain conditions. Compared with collagen, collagen peptides have lower molecular weight, better water solubility, and are easily absorbed by the body. At the same time, co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78
CPCC07K14/78C12P21/06
Inventor 黄雅钦丁丁李雪娟孟兆亭
Owner BEIJING UNIV OF CHEM TECH
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