Ramie germplasm resource hydroponic storage method

A preservation method, ramie technology, applied in planting substrates, botanical equipment and methods, cultivation, etc., can solve problems such as different tissue culture methods, obstacles to the establishment and promotion of vitrification cryopreservation technology systems, and avoid the mixing of germplasm resources , easy to share and use, and prevent underground diseases and pests from invading the effect

Active Publication Date: 2017-06-13
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

However, due to the complexity of ramie germplasm, the tissue culture methods are different with different germplasms. At present, no general tissue culture method has been found, which poses a great obstacle to the establishment and promotion of a general vitrification cryopreservation technology system.

Method used

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  • Ramie germplasm resource hydroponic storage method
  • Ramie germplasm resource hydroponic storage method
  • Ramie germplasm resource hydroponic storage method

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Embodiment Construction

[0043] The present invention is further illustrated in conjunction with specific implementation examples.

[0044] On September 27, 2015, four germplasm young shoots of "Dongkou Qingpima", "Mazuibaima", "Lvzhubai" and "White-skinned Hemp" were taken from the National Germplasm Changsha Ramie Garden, about 25cm long, and each Take 100 germplasms. Cut off the leaves, leave the top 3 to 4 leaves, arrange the stems with a knife, remove the injured part, keep the stems with more than 3 nodes, and obtain the ramie germplasm resources to be preserved ( figure 2 ).

[0045] Disinfect the stem wound with 5% carbendazim, insert it into the Karen soilless cultivation hydroponic device, the length of the tender stem is 1.5-2.5cm, the water in the hydroponic device is clean water (without adding any nutrient solution), and cultivate for 10 days . Change the water and add nutrient solution. The nutrient solution is commercially available, and the amount in the field for the first time i...

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Abstract

The invention provides a ramie germplasm resource hydroponic storage method. As ramie is a unique Chinese commercial crop, germplasm resource safety storage is highly concerned by national and scientific workers all the time, and an important progress is made in ramie germplasm resource storage. In order to store ramie germplasm resources safely and stably, make it convenient for germplasm resource exchange and sharing and overcome defects caused by field planting storage of the ramie germplasm resources, improvement and innovation of an existing storage method are necessary. The ramie germplasm resource hydroponic storage method integrates techniques of storage environments, hydroponic devices, material pretreatment, daily storage management and the like and is available for indoor implementation, safe in germplasm resource storage, simple in process, convenient to manage and low in external environment influences.

Description

technical field [0001] The invention relates to a technology for preserving germplasm resources, in particular to a technology for hydroponic preservation of ramie germplasm resources. Background technique [0002] Ramie (Boehmeria nivea (L.) Gaud.) belongs to Urticaceae (Urticaceae) Ramie (Boehmeria), a perennial plant, known as "Chinese grass". Germplasm resources are strategic resources that human beings rely on for survival and development, and an important material basis for the selection and breeding of new crop varieties, scientific research and production development. Ramie is a unique economic crop in China. Since the 1950s, the safe preservation of germplasm resources has been highly concerned by the state and scientific and technological workers. Important progress has been made in the preservation of ramie germplasm resources. At present, the national germplasm ramie garden with the largest area and the most abundant species and quantity has been built in the wo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00C05G1/00
CPCA01G24/00A01G31/00C05B7/00C05G5/23C05C1/00C05C5/02C05D1/00C05D3/00C05D5/00C05D9/02
Inventor 许英陈建华粟建光戴志刚栾明宝王晓飞唐蜻谭志坚程超华杨泽茂孙志民
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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