Preparation method of rabbit retinitis pigmentosa model

Abstract

The invention discloses a preparation method of a rabbit retinitis pigmentosa model. The preparation method includes steps of applying a certain concentration and dosage of sodium iodate to a rabbit through a method of carotid artery injection, so that the sodium iodate reaches the rabbit's eyes. Through above method, the sodium iodate dosage is few, and toxicity to the whole animal body is small, the animal life safety is not influenced; the method is simple and feasible, good in repeatability, small in individual model difference, and high in success rate. The preparation method provides a reliable model for the study of the retinitis pigmentosa, and also provides an animal model for the drug research and development and preclinical evaluation for treating retinitis pigmentosa.

Description

technical field [0001] The invention relates to the fields of biotechnology and medicine, in particular to a preparation method of a rabbit model of retinitis pigmentosa. Background technique [0002] Retinitis pigmentosa is one of the major causes of blindness today. Retinitis pigmentosa is a progressive degenerative retinal degeneration disease, which mainly involves photoreceptors and pigment epithelium function, and is a hereditary eye disease. At present, there are still no clear and effective therapeutic drugs and methods for retinitis pigmentosa. The development of a stable and reproducible ideal animal model is the key to solving related research and drug preclinical drug evaluation. [0003] Rabbits have large eyeballs, clear and rich background data, and are suitable for ophthalmology research and preclinical drug evaluation. Sodium iodate can damage the retinal pigment epithelium after entering the ocular circulation. At present, rabbits have been used to induc...

AI Technical Summary

Problems solved by technology

At present, rabbits have been used to induce the establishment of retinitis pigmentosa disease models through intravenous injection of sodium iodate. Although the method of intravenous injection of sodium iodate is simpler to operate, because sodium iodate returns with the venous blood, it enters the tissues of the body through the heart and enters the eyes. The sodium iodate in the tissue is only a small part. In order to meet the requirements of retinitis pigmentosa, a higher dose of sodium iodate must be given. Under this condition, obvious systemic toxicity can be seen, especially in the liver, kidney and digestive tract, which can easily cause The failure or failure of one or more organs of an animal, eventually leading to the death of the animal

In the method of intravenous injection of sodium iodate, the dose of retinitis pigmentosa caused by it is similar to the lethal dose of animals, and the physical differences among individual animals lead to low modeling rate, poor repeatability, and easy death of animals

In 1996, Chen Qin et al. reported the injection of 10 mg / ml iodic acid into New Zealand rabbits through the marginal ear vein in the article "Scanning Electron Microscopic Observation of Retinal Pigment Epithelium Induced by Epidermal Growth Factor Therapy with Sodium Iodate" (Ophthalmology Research, No. 4). Sodium (dose 30 mg / kg) induces retinitis pigmentosa, but the dose of this method is low, so repeated injections are required, and the interval between repeated injections has a great influence on the results

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