Preparation method of rabbit retinitis pigmentosa model

A retinal pigment and model technology, applied in the fields of biotechnology and medicine, can solve the problems of large impact on results, low molding rate, animal death, etc., and achieve the effects of simple and feasible operation, small model differences, and good repeatability.

Inactive Publication Date: 2017-06-13
JOINN LAB (SUZHOU) INC
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Problems solved by technology

At present, rabbits have been used to induce the establishment of retinitis pigmentosa disease models through intravenous injection of sodium iodate. Although the method of intravenous injection of sodium iodate is simpler to operate, because sodium iodate returns with the venous blood, it enters the tissues of the body through the heart and enters the eyes. The sodium iodate in the tissue is only a small part. In order to meet the requirements of retinitis pigmentosa, a higher dose of sodium iodate must be given. Under this condition, obvious systemic toxicity can be seen, especially in the liver, kidney and digestive tract, which can easily cause The failure or failure of one or more organs of an animal, eventually leading to the death of the animal
In the method

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  • Preparation method of rabbit retinitis pigmentosa model
  • Preparation method of rabbit retinitis pigmentosa model
  • Preparation method of rabbit retinitis pigmentosa model

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preparation example Construction

[0022] A method for preparing a rabbit model of retinitis pigmentosa is provided, specifically as follows:

[0023] 1. The main reagents in the experiment:

[0024] Fluorescein sodium injection (Guangzhou Baiyunshan Mingxing Pharmaceutical Co., Ltd.), sodium iodate (Sigma, the United States).

[0025] 2. Main instruments

[0026] Optical coherence tomography (Heidelberg, Germany), fundus digital camera (Chongqing Kanghua Ruiming), eye electrophysiology (Diagnosys, USA).

[0027] 3. Experimental animals

[0028] Rabbits were purchased from Qingdao Kangda Biotechnology Co., Ltd.; 8 rabbits, 14 weeks old, weighing 2.5-3 kg.

[0029] (1) Animal feeding and management

[0030] Animal feeding and management were performed in accordance with the SOP of Zhaoyan (Suzhou) New Drug Research Center Co., Ltd., and followed the 8th edition of the Guidelines for the Care and Use of Experimental Animals (Institute of Laboratory Animal Resources, Commission on Life Sciences, National Resea...

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Abstract

The invention discloses a preparation method of a rabbit retinitis pigmentosa model. The preparation method includes steps of applying a certain concentration and dosage of sodium iodate to a rabbit through a method of carotid artery injection, so that the sodium iodate reaches the rabbit's eyes. Through above method, the sodium iodate dosage is few, and toxicity to the whole animal body is small, the animal life safety is not influenced; the method is simple and feasible, good in repeatability, small in individual model difference, and high in success rate. The preparation method provides a reliable model for the study of the retinitis pigmentosa, and also provides an animal model for the drug research and development and preclinical evaluation for treating retinitis pigmentosa.

Description

technical field [0001] The invention relates to the fields of biotechnology and medicine, in particular to a preparation method of a rabbit model of retinitis pigmentosa. Background technique [0002] Retinitis pigmentosa is one of the major causes of blindness today. Retinitis pigmentosa is a progressive degenerative retinal degeneration disease, which mainly involves photoreceptors and pigment epithelium function, and is a hereditary eye disease. At present, there are still no clear and effective therapeutic drugs and methods for retinitis pigmentosa. The development of a stable and reproducible ideal animal model is the key to solving related research and drug preclinical drug evaluation. [0003] Rabbits have large eyeballs, clear and rich background data, and are suitable for ophthalmology research and preclinical drug evaluation. Sodium iodate can damage the retinal pigment epithelium after entering the ocular circulation. At present, rabbits have been used to induc...

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Application Information

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IPC IPC(8): A61K33/18
CPCA61K33/18A61K9/0019
Inventor 姜来生蔡可阳廖琴孙辉业左丛林冯宇霞
Owner JOINN LAB (SUZHOU) INC
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