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Gene PtCYP85A3 of poplar tree and application

A technology of poplar and gene, applied in the direction of application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2017-06-16
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] However, whether the poplar PtCYP85A3 gene involved in the present invention can be overexpressed in plants to increase the content of BRs in plants, and then improve the biomass of plants, fruit yield and enhance the stress resistance of plants has not yet been reported.

Method used

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  • Gene PtCYP85A3 of poplar tree and application
  • Gene PtCYP85A3 of poplar tree and application
  • Gene PtCYP85A3 of poplar tree and application

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Embodiment Construction

[0042] The present invention will be described in detail through specific examples below. These embodiments are provided for a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0043] "Includes" or "comprises" mentioned throughout the specification and claims is an open term, so it should be interpreted as "including but not limited to". The subsequent description in the specification is a preferred implementation mode for implementing the present invention, but the description is for the purpose of the general principles of the specification, and is not intended to limit the scope of the present invention. The scope of protection of the present invention should be defined by the appended claims.

[0044] 1. Materials and methods:

[0045] 1. Medium

[0046] MS 0 Medium: 4.43g / L MS salts, 20g / L sucrose, pH adjusted to 5.8 with NaOH;

[0047] LB medium: 10g / L peptone (Typtone), 5g / L ye...

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Abstract

The invention discloses a gene PtCYP85A3 of a poplar tree and application. The gene can increase the biomass and the fruit yield and improve the salt and drought resistance of a plant. By a specific test, the gene PtCYP85A3 is introduced into a tomato and a Shan xin yang poplar tree to indicate that the gene PtCYP85A3 plays an important role in controlling the growth and the stress resistance of the tomato and the poplar tree and has important application value to the plant genetic engineering and a heredity improvement gene.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a poplar PtCYP85A3 gene and its application in improving plant biomass, fruit yield and enhancing plant stress resistance. Background technique [0002] Poplar is one of the important tree species in the world. It is widely distributed all over the world. It has the characteristics of rapid growth and excellent material. It can provide board and papermaking raw materials. Genetic manipulation of poplar is also relatively easy, so scientists study it as a model woody plant (Taylor G, 2002). Poplar is rich in lignocellulose and perennial, and it is also an ideal raw material tree species for biomass energy. Not long ago, the genome sequencing of poplar has been completed (Tuskan GA et al, 2006). Scientists plan to transform poplar on this basis to make it grow faster and have a smaller canopy. , New species with lower lignin content, which is more conducive to the production of cellulo...

Claims

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Application Information

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IPC IPC(8): C12N15/53C12N9/02C12N15/82A01H5/00
CPCC12N9/0071C12N15/8261C12N15/8273
Inventor 张洪霞晋艳丽唐仁杰于春燕郭笑彤
Owner LUDONG UNIVERSITY
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