A kind of pretreated corn steep liquor and its direct injection method in livestock and poultry feed
A corn steep liquor and pretreatment technology, applied in animal feed, animal feed, application, etc., can solve the problems of unfavorable corn steep liquor feed composition application, lower feed utilization rate, high water content of corn steep liquor, etc., and prevent the loss of feed nutrients , Improve the production efficiency of the enterprise, and reduce the water content
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Embodiment 1
[0041] A pretreatment method for corn steep liquor, comprising the steps of:
[0042] (1) Add 1mol / L NaOH solution to the corn steep liquor, stir and process, and adjust the pH of the corn steep liquor to 5.3;
[0043] (2) Add liquid phytase to the corn steep liquor after pH adjustment, based on the enzyme activity of 5000U / ml, when the phytase acts, the temperature is 35°C, the rotation speed is 35r / min, the stirring reaction time is 3h; the addition amount is 1.0 L / m 3 meter, stir and mix;
[0044] (3) Insert cultured Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Aspergillus niger (Aspergillus niger) respectively into the corn steep liquor after enzymolysis obtained in step (2) to carry out fermentation culture; The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Aspergillus niger (Aspergillus niger) inoculation sequence and time: first inoculate Bacillus amyloliquefaciens, stirring speed 150r / min, aerobic fermentation 8h, then inoculate Aspergill...
Embodiment 2
[0047] A pretreatment method for corn steep liquor, comprising the steps of:
[0048] (1) Add 1mol / L NaOH solution to the corn steep liquor, stir and process, and adjust the pH of the corn steep liquor to 5.2;
[0049] (2) Add liquid phytase to the corn steep liquor after pH adjustment, based on the enzyme activity of 5000U / ml, when the phytase acts, the temperature is 40°C, the rotation speed is 25r / min, the stirring reaction time is 3h; the addition amount is 0.8 L / m 3 meter, stir and mix;
[0050] (3) Insert cultured Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Aspergillus niger (Aspergillus niger) respectively into the corn steep liquor after enzymolysis obtained in step (2) to carry out fermentation culture; The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Aspergillus niger (Aspergillus niger) inoculation sequence and time: first inoculate Bacillus amyloliquefaciens, stirring speed 100r / min, after aerobic fermentation for 12h, then inoculat...
Embodiment 3
[0053] A pretreatment method for corn steep liquor, comprising the steps of:
[0054] (1) Add 1mol / L NaOH solution to the corn steep liquor, stir and process, and adjust the pH of the corn steep liquor to 5.3;
[0055] (2) Add liquid phytase to the corn steep liquor after pH adjustment, based on the enzyme activity of 5000U / ml, when the phytase acts, the temperature is 37°C, the rotation speed is 30r / min, the stirring reaction time is 2h; the addition amount is 0.5 L / m 3 meter, stir and mix;
[0056] (3) Insert cultured Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Aspergillus niger (Aspergillus niger) respectively into the corn steep liquor after enzymolysis obtained in step (2) to carry out fermentation culture; The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Aspergillus niger (Aspergillus niger) inoculation sequence and time: first inoculate Bacillus amyloliquefaciens, stirring speed 150r / min, aerobic fermentation 8h, then inoculate Aspergill...
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