The invention discloses a method for preparing 
delta 1-11 alpha,17 alpha-dihydroxyprogesterone with a one-pot 
continuous fermentation process, and belongs to the technical field of intermediate preparation. The method comprises the following specific steps: (1) inoculating slant culture of 
arthrobacter simplex into a first culture medium, performing culture for 20-30 hours, adding ground 17 alpha-
hydroxyprogesterone, and performing transformation; (2) inoculating 
fermentation liquor after finishing the step (1) into a second culture medium, inoculating slant culture of 
aspergillus ochraceus into the second culture medium, performing culture, and performing post-treatment to obtain 
delta 1-11 alpha,17 alpha-dihydroxyprogesterone. 
Delta 1-11 alpha,17 alpha-dihydroxyprogesterone is prepared with the one-pot 
continuous fermentation process, so that a step of filtering, extracting, refining and 
drying a 
dehydrogenation material is reduced, 
discharge of 50% pollutants is reduced, 
environmental pressure is reduced, the time is saved, the yield is increased, the efficiency is improved, the process is simplified, the whole reaction operation is convenient, and the method is suitable for industrialized popularization and application.