Pretreated corn steep liquor and direct spray using method thereof in livestock and poultry feed
A livestock and poultry feed, corn steep liquor technology, applied in animal feed, animal feed, application, etc., can solve the problems of unfavorable corn steep liquor feed composition application, high water content of corn steep liquor, and reduced feed utilization rate, so as to prevent feed nutrients The effect of loss, water content reduction, and improvement of enterprise production efficiency
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Embodiment 1
[0041] A pretreatment method for corn steep liquor, comprising the steps of:
[0042] (1) Add 1mol / L NaOH solution to the corn steep liquor, stir and process, and adjust the pH of the corn steep liquor to 5.3;
[0043] (2) Add liquid phytase to the corn steep liquor after pH adjustment, based on the enzyme activity of 5000U / ml, when the phytase acts, the temperature is 35°C, the rotation speed is 35r / min, the stirring reaction time is 3h; the addition amount is 1.0 L / m 3 meter, stir and mix;
[0044] (3) Insert cultured Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Aspergillus niger (Aspergillus niger) respectively into the corn steep liquor after enzymolysis obtained in step (2) to carry out fermentation culture; The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Aspergillus niger (Aspergillus niger) inoculation sequence and time: first inoculate Bacillus amyloliquefaciens, stirring speed 150r / min, aerobic fermentation 8h, then inoculate Aspergill...
Embodiment 2
[0047] A pretreatment method for corn steep liquor, comprising the steps of:
[0048] (1) Add 1mol / L NaOH solution to the corn steep liquor, stir and process, and adjust the pH of the corn steep liquor to 5.2;
[0049] (2) Add liquid phytase to the corn steep liquor after pH adjustment, based on the enzyme activity of 5000U / ml, when the phytase acts, the temperature is 40°C, the rotation speed is 25r / min, the stirring reaction time is 3h; the addition amount is 0.8 L / m 3 meter, stir and mix;
[0050] (3) Insert cultured Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Aspergillus niger (Aspergillus niger) respectively into the corn steep liquor after enzymolysis obtained in step (2) to carry out fermentation culture; The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Aspergillus niger (Aspergillus niger) inoculation sequence and time: first inoculate Bacillus amyloliquefaciens, stirring speed 100r / min, after aerobic fermentation for 12h, then inoculat...
Embodiment 3
[0053] A pretreatment method for corn steep liquor, comprising the steps of:
[0054] (1) Add 1mol / L NaOH solution to the corn steep liquor, stir and process, and adjust the pH of the corn steep liquor to 5.3;
[0055] (2) Add liquid phytase to the corn steep liquor after pH adjustment, based on the enzyme activity of 5000U / ml, when the phytase acts, the temperature is 37°C, the rotation speed is 30r / min, the stirring reaction time is 2h; the addition amount is 0.5 L / m 3 meter, stir and mix;
[0056] (3) Insert cultured Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Aspergillus niger (Aspergillus niger) respectively into the corn steep liquor after enzymolysis obtained in step (2) to carry out fermentation culture; The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Aspergillus niger (Aspergillus niger) inoculation sequence and time: first inoculate Bacillus amyloliquefaciens, stirring speed 150r / min, aerobic fermentation 8h, then inoculate Aspergill...
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