Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kits for the detection of juvenile osteoporosis

An osteoporosis and kit technology, applied in the field of detection, can solve the problem of not being able to popularize and use in a large area, and achieve the effect of convenient, timely and accurate genetic diagnosis and detection, and reducing the deformity rate.

Active Publication Date: 2020-06-16
SUZHOU UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the need for special instruments, this method cannot be widely used in some poor areas.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kits for the detection of juvenile osteoporosis
  • Kits for the detection of juvenile osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment one: see figure 1 As shown, Example 1 screening of juvenile osteoporosis patients and screening of gene variation in normal people:

[0027] 1. Research objects: Case samples were collected from the First Affiliated Hospital of Soochow University and the Second Affiliated Hospital of Soochow University, as follows:

[0028] Juvenile osteoporosis group: 150 juvenile osteoporosis patients admitted to the orthopedics department of the hospital were selected, 25 males and 25 females, the youngest was 20 years old and the oldest was 50 years old. Inclusion criteria: in line with the "Recommended Diagnostic Criteria for Osteoporosis in China". No obvious heart, liver, kidney, pulmonary insufficiency, no various endocrine diseases causing secondary osteoporosis, no tumor, diabetes and other serious diseases that interfere with bone metabolism.

[0029] Normal group: select 20 healthy volunteers aged 8-15, 10 males and 10 males.

[0030] There was no significant d...

Embodiment 2

[0034] Embodiment two: PCR amplification identification

[0035] (1) Primer design

[0036]PCR amplification primers were designed according to the sequence of DKK-1 gene AF177394.1 in Genbank, and synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd. The specific primer sequences are shown in SEQ ID NO: 1-2. Housekeeping genes were used as controls.

[0037] The PCR reaction system is: DNA template 1 μl, upstream and downstream primers 0.5 μl each, PCR Mix 12.5 μl, ultrapure water supplemented to 25 μl;

[0038] PCR reaction conditions: pre-denaturation at 95°C for 5 minutes, entering into a cycle of 95°C for 60s, 57.5°C for 1min and 72°C for 1min, cycling 35 times, and finally extending at 72°C for 5min.

[0039] The polymerase chain reaction system was purchased from Shanghai Sangong.

[0040] (2) Acquisition of templates: the blood DNA of another 30 groups of juvenile osteoporosis patients and 10 groups of normal people was extracted, and the speci...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit for detecting juvenile osteoporosis. According to the kit, an obvious mutant site which is a site 1020 is discovered to exist in a DKK1 gene in a patient suffering from the juvenile osteoporosis and a normal crowd through genetic comparison. Through detecting the mutant site, particularly provided one pair of specific primer pairs, a human body which suffers from the juvenile osteoporosis can be specifically identified. Compared with the method in the prior art, a detection method has the advantages of being quick for detection, accurate and quick, and is suitable to popularize and use.

Description

technical field [0001] The invention belongs to the detection field and relates to a kit for juvenile osteoporosis detection. Background technique [0002] Osteoporosis (osteoporosis) is a systemic bone disease in which bone density and bone quality decrease due to various reasons, bone microstructure is destroyed, bone fragility increases, and fractures are prone to occur. In the early years, it was generally believed that osteopenia was osteoporosis, while in the United States, fractures in the elderly were considered osteoporosis. It was not until the Third International Osteoporosis Symposium held in Denmark in 1990 and the Fourth International Osteoporosis Symposium held in Hong Kong in 1993 that osteoporosis had a clear definition and was recognized worldwide. Background: Primary osteoporosis is a systemic skeletal disease characterized by decreased bone mass and degeneration of bone microstructure, resulting in increased bone fragility and prone to fractures. Every ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 裴育芳张垒张永红
Owner SUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com