Method for preparing pork powder standard substance with clenbuterol

A standard material, pork meal technology, applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of good uniformity and stability, easy to popularize, and simple to operate

Active Publication Date: 2017-06-20
NAT INST OF METROLOGY CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Announcement No. 235 of the Ministry of Agriculture of my country clearly stipulates that clenbuterol is a prohibited drug and shall not be detected in animal food

Method used

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  • Method for preparing pork powder standard substance with clenbuterol
  • Method for preparing pork powder standard substance with clenbuterol
  • Method for preparing pork powder standard substance with clenbuterol

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Embodiment Construction

[0027] Preferred embodiments of the present invention are described below, and it should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0028] In this example, the raw material first needs to be obtained, that is, a pork sample containing clenbuterol.

[0029] Source: Healthy pigs (Yorkshire Large White) fed clenbuterol.

[0030] Positive feeding plan: body weight 60-75kg, mix an appropriate amount of medicine into one day's pig feed (corn feed made by the farm), and stir evenly. Feed and drinking water were provided ad libitum, fed continuously for 28-30 days, slaughtered on the day of drug withdrawal, and pork samples were obtained.

[0031] 1. Pork sample pretreatment:

[0032] (1) Take the positive pig muscle part, remove residual fat, fascia, etc., and cut into pieces. Freeze and grind until pulverized, as a raw material.

[0033] (2) Take...

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Abstract

The invention discloses a method for preparing a pork powder standard substance with clenbuterol. The method comprises the following steps: performing simulated feeding so as to obtain a pork raw material polluted by clenbuterol, crushing a sample, directly mixing with water in a proper ratio, preparing freeze-dried pork by using a freeze-drying method directly without homogenation, and implementing steps of grinding, screening, uniform mixing, radiation and the like, thereby obtaining freeze-dried pork powder in good uniformity. Value confirmation, uniformity inspection and stability monitoring of standard substances are implemented by using a liquid chromatogram isotope dilution mass spectrometry (LC-ID-MS/MS) method for verifying accuracy through international comparison. The pork powder standard substance with clenbuterol, which is prepared by using the method, is good in uniformity and stability, and can be applied to analysis method evaluation for residue detection on clenbuterol in pork, quality control in analysis process and laboratory capability evaluation.

Description

technical field [0001] The invention relates to a method for preparing a pork powder matrix standard substance containing clenbuterol (clenbuterol). The clenbuterol standard substance in pork powder is a measurement standard and is mainly used for calibrating measuring instruments and evaluating measurement methods Accuracy. Background technique [0002] Clenbuterol hydrochloride (Clenbuterol), English common name: Clenbuterol HCl; chemical name: a[(tert-butylamino)methyl]-4-amino-3,5-dichlorobenzyl alcohol hydrochloride; 1-( 4-amino-3, 5-dichloro-phenyl)-2-(tert-butylamino)ethanol; molecular formula: C 12 h 18 Cl 2 N 2 O; molecular weight: 277.19; CAS registration number: 37148-27-9; hydrochloride CAS registration number: 21898-19-1, the structural formula is as follows figure 1 shown. [0003] Clenbuterol hydrochloride is a synthetic β2-adrenergic receptor agonist. People who eat high-content residual food can cause symptoms such as palpitations, chest tightness, tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 李秀琴张庆合阚莹李红梅郑思珩
Owner NAT INST OF METROLOGY CHINA
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