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A method for tissue culture and rapid propagation of folk medicine and food homologous plant Chicanyus chinensis

A technology of tissue culture and fast propagation of medicinal and food homologous plants, applied in the field of plant tissue culture, can solve the problems of no tissue culture technology of red vine, no patent application for tissue culture fast propagation of red vine, etc., and achieve a short cycle. , low cost, simple process effect

Inactive Publication Date: 2019-03-15
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the tissue culture technology of Chicanyus at home and abroad, and there is no application for a patent for tissue culture and rapid propagation of Chicanyus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Collection of explants: In the field, select the middle and upper part of the Cane vine plant that grows robustly and has no obvious diseases, and the canes with joints that are sunny and full are used as explants. Immediately after collection, water retention and moisturizing treatment are carried out and brought back to the laboratory in time. .

[0021] (2) Induction culture: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 10 seconds, and rinsed with sterile water for 3 times After drying the water with sterile filter paper, put it in 0.1% mercuric chloride solution for disinfection for 10 minutes, rinse it with sterile water for 3 times, dry it with sterile filter paper, cut it into 2.0-2.5cm canes with joints and cut them into Inoculate it into the induction medium and place it at 25°C for 25 days in total darkness to induce ...

Embodiment 2

[0027] (1) Collection of explants: In the field, select the middle and upper part of the Cane vine plant that grows robustly and has no obvious diseases, and the canes with joints that are sunny and full are used as explants. Immediately after collection, water retention and moisturizing treatment are carried out and brought back to the laboratory in time. .

[0028] (2) Induction culture: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 23 seconds, and rinsed with sterile water for 4 times After drying the water with sterile filter paper, put it in 0.1% mercuric chloride solution for disinfection for 15 minutes, rinse it with sterile water for 4 times, dry it with sterile filter paper, cut it into 2.0-2.5cm canes with joints and cut them into Inoculate into the induction medium and place it at 27°C for 28 days in total darkness to induce the...

Embodiment 3

[0034](1) Collection of explants: In the field, select the middle and upper part of the Cane vine plant that grows robustly and has no obvious diseases, and the canes with joints that are sunny and full are used as explants. Immediately after collection, water retention and moisturizing treatment are carried out and brought back to the laboratory in time. .

[0035] (2) Induction culture: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 15 seconds, and rinsed with sterile water for 5 times After drying the water with sterile filter paper, put it in 0.1% mercuric chloride solution for disinfection for 20 minutes, rinse it with sterile water for 5 times, dry it with sterile filter paper, cut it into 2.0-2.5cm canes with joints and cut them into Inoculate it into the induction medium and place it at 28°C for 30 days in total darkness to induce t...

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Abstract

The invention relates to a plant tissue culturing method in an agricultural biotechnology, and in particular relates to a tissue-culturing rapid-propagating method for a civilian medicinal and edible plant, erythropalum scandens. The method sequentially comprises the following steps of acquisition of explant of the erythropalum scandens, induction culture of the explant, subculture of adventitious buds, rooting culture of the adventitious buds and transplanting of test-tube plantlets. An induction medium applied to induced culture of the explant comprises an MS culture medium, 4.0-6.0mg / L 6-BA, 0.5-1.0mg / L NAA, 20-30g / L cane sugar, 3.5-4.0g / L agar and 0.5-1.0g / L active carbon, and pH value of the induction medium is 5.4-5.8. According to the method provided by the invention, the transplanting survival rate of the civilian medicinal and edible plant, erythropalum scandens is over 91%.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of the folk medicine and food homologous plant Chicane vine. Background technique [0002] Erythropalum scandens BL., also known as Erythropalum scandens BL., is a woody vine of the genus Erythropalum scandens in the family Ivyaceae. It is mainly distributed in Guangxi, Guangdong, Yunnan, and Guizhou in my country. It is common in low mountains, hills or mountain streams, valleys, dense or sparse forests, forest margins or thickets. Chi Cang Teng has the effect of clearing away heat and dampness, expelling wind and activating blood circulation, and is often used in the treatment of hepatitis, tumor, urethritis, acute nephritis and other diseases in Guangxi folk. In addition, Chicanterus is a kind of leafy vegetables in wild vegetables. It has a unique taste such as freshness, mellowness, and aroma....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 梁钧淞杨业容莫昭展韦敏
Owner YULIN NORMAL UNIVERSITY
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