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A kind of in vitro culture and rapid propagation method of mantle orchid

A technology of in vitro culture and Trifolium pratense, applied in the field of plant tissue culture, can solve the problems of not being widely developed and applied, low reproductive efficiency, sharp reduction in quantity, etc. Effect

Inactive Publication Date: 2018-11-27
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, mantle orchids are mainly propagated by ramets, the reproductive efficiency is low, and seedlings are scarce, which leads to their not being widely developed and applied. deteriorating, the number has dropped sharply, and it is in a near-endangered state, so it is necessary to establish a rapid propagation system for in vitro culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Collection of explants: In the spring of 2013, when new buds germinated, select robust cylindrica plants without obvious diseases in the field, use a scalpel to cut the newly-born vegetative buds with unopened leaf sheaths from the base, and perform simple water retention and moisturizing treatments Then bring it back to the laboratory for disinfection treatment in time.

[0022] (2) Induction of rhizomes: Rinse the explants overnight under running water, peel off the outer leaf sheaths until only two leaf sheaths are left, and place them on a clean bench and sterilize them in 0.1% mercury solution for 5 minutes. After washing twice with sterile water, peel off a layer of leaf sheath, and then put it in 0.1% mercury solution for disinfection for 1 minute. After rinsing with sterile water for 3 times, peel off the last layer of leaf sheath and rinse with sterile water for 5 times. Use sterile filter paper to absorb the water on the surface of the explant, and cut the ex...

Embodiment 2

[0030] (1) Explant collection: In the spring of 2014, when the sprouts of Phyllanthus spp. sprout, select robust plants with no obvious diseases in the field, use a scalpel to cut the newly-born nutrient buds with unopened leaf sheaths from the base, and perform water retention and moisturizing treatments in time Take it back to the laboratory for disinfection.

[0031] (2) Induction of rhizomes: Rinse the explants overnight under running water, peel off the outer leaf sheaths until only two leaf sheaths remain, and place them on a clean bench and sterilize them in a 0.1% mercury solution for 8 minutes. After rinsing with sterile water twice, peel off a layer of leaf sheath, and then put it in a 0.1% mercury solution for disinfection for 2 minutes. After rinsing with sterile water for 4 times, peel off the last layer of leaf sheath and rinse with sterile water for 6 times. Use sterile filter paper to absorb the water on the surface of the explant, and cut the explant into 0.3~0.5...

Embodiment 3

[0039] (1) Explant collection: In the spring of 2015, when the sprouts of Phyllanthus sp Take it back to the laboratory for disinfection.

[0040] (2) Induction of rhizomes: Rinse the explants overnight under running water, peel off the outer leaf sheaths until only two leaf sheaths are left, and place them on a clean bench and sterilize them in a 0.1% mercury solution for 10 minutes. After rinsing with sterile water 3 times, peel off a layer of leaf sheath, and then put it in 0.1% mercury solution for 3 minutes. After rinsing with sterile water for 5 times, peel off the last layer of leaf sheath and rinse with sterile water for 7 times. Use sterile filter paper to absorb the water on the surface of the explant, and cut the explant into 0.3~0.5 lengthwise with a sterile scalpel. The shoot point of about cm is inoculated into the induction medium and cultured in the dark for 120 days to induce the formation of rhizomes with a length of about 2 to 3 cm. The pollution rate is less ...

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PUM

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Abstract

The invention relates to a method of plant tissue culture in the agricultural biotechnology, in particular to a hippeophyllum sinicum in-vitro culture and rapid propagation method which comprises the following steps: acquisition of an explant of a hippeophyllum sinicum plant, induction culture of the explant, multiplication culture of a rhizome, differentiation culture of the rhizome, strong seedling culture, seedling adaptation and seedling transplantation. An explant induction culture medium for an explant induction culture operation is prepared from a KC culture medium, 0.5-1.0mg / L of 6-BA, 0.01-0.05mg / L of ZT, 0.1-1.0mg / L IBA, 15-25g / L of sucrose, 3.5-5.0g / L of agar and 30-50ml / L of a coconut juice, and a pH value of the explant induction culture medium is 5.4-5.8. According to the method, by selecting a new vegetative bud of hippeophyllum sinicum as the explant and performing the steps of induction culture, multiplication culture, differentiation culture, strong seedling culture, seedling adaptation and seedling transplantation, the induction rate of the explant reaches 68 percent or above, the survival rate of the explant reaches 94 percent or above, tissue culture and rapid propagation of the hippeophyllum sinicum are realized, and thus the purpose of the invention is achieved.

Description

Technical field [0001] The invention relates to a method for culturing plant tissues in agricultural biotechnology, in particular to a method for in vitro culture and rapid propagation of Phyllanthus. Background technique [0002] "Bamboo has knots and sips flowers. Plum has flowers and sips leaves, pine has leaves and scents. Only orchids have both." This is people's personal evaluation of orchids known as "gentleman flowers." Orchid not only has the supremacy in the appreciation of natural beauty, but also covers the excellent character of all flowers and becomes the most perfect "personality flower", but in recent years, due to people's inexhaustible mining, its number has decreased sharply, including Many orchid plants including Hippeophyllum sinicum are endangered. Tetraphyllum is a kind of epiphytic orchid of Hippeophy Schltr. of Orchidaceae (Orchidaceae). There are about 6 species of Phyllanthus in Malaysia, Indonesia, the Philippines, New Guinea, Solomon Islands and my ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G22/63
CPCA01G22/00A01H4/001A01H4/008
Inventor 梁钧淞杨业容韦敏
Owner YULIN NORMAL UNIVERSITY
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