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Rapid detection method for nitrification effect of nitrobacteria

A technology of nitrifying bacteria and detection methods, applied in biochemical equipment and methods, determination/inspection of microorganisms, material analysis by observing the influence of chemical indicators, etc. Simple, fast results

Inactive Publication Date: 2017-07-04
无锡市中渔健宝生物有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the prior art, the actual content level of nitrite in the water body can be determined according to the national standard GB / T 7493-1987 Spectrophotometric method for water quality nitrosated nitrogen, and various forms and specifications of water quality produced at home and abroad can also be used. However, these methods are not methods for directly measuring the nitrification effect of nitrifying bacteria

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  • Rapid detection method for nitrification effect of nitrobacteria
  • Rapid detection method for nitrification effect of nitrobacteria
  • Rapid detection method for nitrification effect of nitrobacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0061] Nitrous nitrogen NO in field aquaculture water 2 -N content estimation:

[0062] Firstly, the standard working solution of nitroso-state nitrogen with a concentration of 2ppm was prepared, and then gradually diluted by 50% with pure water to obtain 5 different concentrations of nitroso-state nitrogen standard solution of 2ppm, 1ppm, 0.5ppm, 0.25ppm and 0.125ppm. After colorimetry, the colorimetric tube spectra of different nitroso nitrogen concentrations can be obtained.

[0063] At the same time, when the aquaculture water to be detected is compared with the standard working solutions of the above five configurations at the same time, according to the comparison of color development, it can be known that the concentration of the aquaculture water is between 0.5ppm and 0.25ppm. In order to further determine the concentration range, the 0.5ppm standard working solution was diluted with water into 0.3ppm and 0.4ppm standard dilutions, and then compared with the aquacultu...

Embodiment 2

[0065] Rapid detection test of nitrifying bacteria on the nitrification effect of nitroso nitrogen standard working solution:

[0066] Use 6 empty bottles of mineral water, add 100, 200, 300, 400, 500 and 600mL of 2.02ppm nitroso nitrogen standard working solution to each bottle, then add 1mL of nitrifying bacteria to each bottle, mix well, and form The nitration reaction system was immediately put into a constant temperature box, adjusted to 25°C, left to stand, and maintained for 22 hours to carry out the nitration reaction. After the reaction, the colorimetric detection and nitrification effect evaluation were carried out. The results showed that the nitration reaction solution in the previous 500mL bottle was colorless after colorimetry, but the 600mL reaction bottle was reddish after colorimetry. This bottle was diluted with a standard working solution. The colorimetric method is compared and detected, and the calculation determines that the nitrite removal rate of this b...

Embodiment 3

[0068] Rapid detection test of nitrification effect of nitrifying bacteria in aquaculture water

[0069] Take aquaculture water, the water quality measuring instrument measures its nitroso state nitrogen to be 2.95ppm, and use nitrifying bacteria to carry out the nitrite reduction effect test on this aquaculture water: use 6 empty mineral water bottles, add in aquaculture water 100mL, 200mL, 300mL, 400mL, 500mL and 600mL, then add 1g of nitrifying bacteria to each bottle and mix well to form a different volume gradient nitrification reaction system. Then put it into a constant temperature box, adjust the constant temperature at 35°C, let it stand still, and carry out the nitration reaction for 16 hours. The results of the colorimetric test showed that the reaction liquids of the 100mL, 200mL and 300mL nitration reaction bottles were colorless, the reaction liquids of the 400mL nitration reaction bottles were pink, and the reaction liquids of the 500mL and 600mL nitration react...

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Abstract

The invention discloses a rapid detection method for nitrification effect of nitrobacteria. The method comprises the following steps: 1) preparing a nitrite liquid, which comprises preparation of nitrite nitrogen NO2-N standard operating fluid or taking of nitrite-containing aquatic product culture water; 2) preparing nitrated reaction systems with different volume gradients; 3) performing the nitrification for 16-22 hours in a thermotank at the temperature of 25-35 DEG C; 4) preparing a colorimetric reagent; 5) performing colorimetric detection; and 6) performing assessment on the colorimetric result and the nitrification effect. The method is simple, visual and practical, the obtained result is rapid; direct and rapid detection is carried out on the nitrification effect of nitrobacteria by a culture filed, whether the nitrification effect is generated on the nitrobacteria or not can be qualitative, the level of the quantification nitrification effect is carried out, and the method has guidance meaning for monitoring aquatic product culture water quality and solving the problem of the aquatic product culture.

Description

technical field [0001] The invention belongs to the field of aquaculture, and specifically refers to a rapid detection method for the nitrification effect of nitrifying bacteria. Background technique [0002] Nitrifying bacteria is an environmental ecological microorganism, which plays a huge role in promoting the nitrogen cycle of aquatic ecosystems and maintaining a healthy aquaculture environment. In recent years, with the increasing scale and intensification of aquaculture, the problem of water pollution has become increasingly serious. Among the many pollutants formed in aquaculture waters, nitrite is particularly prominent and the most harmful, and it has significant toxicity to farmed animals. If the content of nitrite in the water body is high, it indicates that the water quality will deteriorate, which will cause poisoning of farmed animals, and many disease symptoms will occur as a result, and severe cases will lead to death. The general law of aquaculture is tha...

Claims

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Application Information

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IPC IPC(8): C12Q1/02G01N21/78
CPCC12Q1/02G01N21/78
Inventor 邹益东邹苏燕薛宏基邢华刘凤琴
Owner 无锡市中渔健宝生物有限公司
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