A kit for screening cancer brain metastases

A kit and brain transfer technology, applied in the field of molecular biology, can solve the problem that the function of lnc-MMP2-2 has not been reported yet.

A kit and brain transfer technology, applied in the field of molecular biology, can solve the problem that the function of lnc-MMP2-2 has not been reported yet.

CN106916900BActive Publication Date: 2021-04-16成都医学院第一附属医院
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  • A kit for screening cancer brain metastases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 The relationship between the expression level of long non-coding RNA lnc-MMP2-2 and patients with brain metastases from lung cancer

[0026] 1. Experimental materials

[0027] Lung adenocarcinoma tissue chip microarray (XT14-051): purchased from Shanghai Xinchao Biotechnology Co., Ltd., including 19 cases of normal lung tissue, 14 cases of in situ lung adenocarcinoma tissue (non-metastasis) and brain metastasis tissue of lung adenocarcinoma 11 cases;

[0028] FAM-lncRNA-MMP2-2 nucleic acid probe was designed and synthesized by Shanghai Gefan Biotechnology Co., Ltd., the sequence is: 5'-FAM-ACCCTAGGCTGCAGGCTCCTGCTTTGGGCT-3';

[0029] In situ hybridization kit (C004) was purchased from Shanghai Gefan Biotechnology Co., Ltd., the kit contains: 10X proteinase K, prehybridization solution, acetic anhydride, triethanolamine aqueous solution pH8.0, 20XSSC pH4.5, 20X SSC pH7.5 ;

[0030] Other self-prepared reagents (glycine, formamide, hydrochloric acid, xylene, 3...

Embodiment 2

[0060] Example 2 The composition and method of use of the kit for detecting the expression level of long-chain non-coding RNA lnc-MMP2-2 according to the present invention

[0061] 1. The composition of the kit

[0062] FAM-lncRNA-MMP2-2 nucleic acid probe, the sequence is:

[0063] 5'-FAM-ACCCTAGGCTGCAGGCTCCTGCTTTGGGCT-3';

[0064] For in situ hybridization: 10X proteinase K, prehybridization solution, acetic anhydride, triethanolamine aqueous solution pH8.0, 20XSSC pH4.5, 20X SSC pH7.5.

[0065] DEPC water and DAPI dye, antifade mounting medium.

[0066] 2. How to use the kit

[0067] The brain tissue of the sample to be tested was used to detect the expression level of lncRNA-MMP2-2 according to the in situ hybridization method.

[0068] For lung cancer patients with suspected metastases, tissue from abnormal parts of the brain and lung tissue can be collected to compare the expression level of lncRNA-MMP2-2, and then evaluate the possibility of lung cancer brain metast...

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Abstract

The invention discloses a kit for screening cancer brain metastases. The invention also discloses the use of the reagent for detecting the expression level of long-chain non-coding RNA lnc-MMP2-2 in the preparation of reagents for screening cancer brain metastasis. By detecting the expression level of lnc-MMP2-2, the kit of the present invention can determine the risk of brain metastases of lung cancer in the subject, can be used for auxiliary diagnosis of brain metastases of lung cancer, and provide effective basis for patients to take relevant treatment measures or decision-making , the clinical application prospect is good.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to a kit for screening cancer brain metastases. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. In the morbidity and mortality of malignant tumors, lung cancer accounts for 13.0% and 19.4% respectively, ranking first among malignant tumors. In addition, due to factors such as China's aging population and younger smoking population, the population base of lung cancer patients is still rising. Brain Metastases is the most common complication of lung cancer. With the increase of the incidence of lung cancer and the prolongation of the survival period of lung cancer patients, the incidence of brain metastases of lung cancer is increasing day by day. About 17% to 57% of lung cancer patients Brain metastases occur during the process, especially in lung adenocarcinoma. Brain metastases from lung cancer account for 40% to 70% of intracran...

Claims

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Application Information

Patent Timeline
16 Apr 2021
Publication
CN106916900B
IPC
C12Q1/6886
CPC
C12Q1/6886; C12Q2600/158; C12Q2600/178
Inventors
吴东明; 许颖