Method for detecting benzene series in biological sample blood
A measurement method and technology of biological samples, which are applied in the field of determination of benzene series in biological sample blood, can solve the problems of complex blood components and physical and chemical properties, and the method of benzene series is not suitable for blood detection, etc., so as to avoid inaccurate quantification, good separation effect
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Embodiment 1
[0077] Take 5 mL of blood from Wang, a resident of a key polluted area, and put it into a 40 mL sample bottle according to the above method, followed by adding 200 μL of isotope internal standard solution, a drop of antifoaming agent and 20 mL of pure water. Seal the vial and shake it up and down 20 times. Then fill the sample bottle with pure water and seal the sample bottle. Shake in an ultrasonic oscillator for 1 min, and measure on the machine. The measurement results are shown in Table 5.
[0078]
[0079] It can be seen that all six benzene series were detected in Wang's blood, among which the concentrations of benzene and toluene in the sample were higher, and the concentrations of ethylbenzene, m / p-xylene and o-xylene were lower. It is suggested that the residents should pay attention to prevent long-term direct or indirect contact with benzene series, and should take a series of prevention and control measures to protect their physical and mental health.
Embodiment 2
[0081] Take 5 mL of blood from Si, a resident in a key polluted area, and add it to a 40 mL sample bottle according to the above method, followed by adding 200 μL of isotope internal standard solution, a drop of antifoaming agent and 20 mL of pure water. Seal the vial and shake it up and down 20 times. Then fill the sample bottle with pure water and seal the sample bottle. Shake in an ultrasonic oscillator for 1 min, and measure on the machine. The measurement results are shown in Table 6.
[0082]
[0083] Note:
[0084] It can be seen that benzene and o-xylene were detected in Si's blood, and the concentration of benzene in the sample was relatively high, while the concentration of o-xylene was low. It is suggested that the residents should pay attention to prevent long-term direct or indirect contact with benzene series, and should take a series of prevention and control measures to protect their physical and mental health.
Embodiment 3
[0086] Take 5 mL of blood from Yao, a resident of a key polluted area, and put it into a 40 mL sample bottle according to the above method, followed by adding 200 μL of isotope internal standard solution, a drop of antifoaming agent and 20 mL of pure water. Seal the vial and shake it up and down 20 times. Then fill the sample bottle with pure water and seal the sample bottle. Shake in an ultrasonic oscillator for 1 min, and measure on the machine. The measurement results are shown in Table 7.
[0087]
[0088] Note:
[0089] It can be seen that benzene and toluene were detected in Yao's blood, and the concentration of benzene in the sample was relatively high, while the concentration of toluene was relatively low. It is suggested that the residents should pay attention to prevent long-term direct or indirect contact with benzene series, and should take a series of prevention and control measures to protect their physical and mental he...
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