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Method for detecting benzene series in biological sample blood

A measurement method and technology of biological samples, which are applied in the field of determination of benzene series in biological sample blood, can solve the problems of complex blood components and physical and chemical properties, and the method of benzene series is not suitable for blood detection, etc., so as to avoid inaccurate quantification, good separation effect

Inactive Publication Date: 2017-07-14
CHENGDU TME SOFTWARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many detection methods for benzene series in air and water, and each method has been well known by the public. Bacteria-based methods are not suitable for testing blood
There is no report on the detection technology of benzene series in blood of biological samples

Method used

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  • Method for detecting benzene series in biological sample blood
  • Method for detecting benzene series in biological sample blood
  • Method for detecting benzene series in biological sample blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Take 5 mL of blood from Wang, a resident of a key polluted area, and put it into a 40 mL sample bottle according to the above method, followed by adding 200 μL of isotope internal standard solution, a drop of antifoaming agent and 20 mL of pure water. Seal the vial and shake it up and down 20 times. Then fill the sample bottle with pure water and seal the sample bottle. Shake in an ultrasonic oscillator for 1 min, and measure on the machine. The measurement results are shown in Table 5.

[0078]

[0079] It can be seen that all six benzene series were detected in Wang's blood, among which the concentrations of benzene and toluene in the sample were higher, and the concentrations of ethylbenzene, m / p-xylene and o-xylene were lower. It is suggested that the residents should pay attention to prevent long-term direct or indirect contact with benzene series, and should take a series of prevention and control measures to protect their physical and mental health.

Embodiment 2

[0081] Take 5 mL of blood from Si, a resident in a key polluted area, and add it to a 40 mL sample bottle according to the above method, followed by adding 200 μL of isotope internal standard solution, a drop of antifoaming agent and 20 mL of pure water. Seal the vial and shake it up and down 20 times. Then fill the sample bottle with pure water and seal the sample bottle. Shake in an ultrasonic oscillator for 1 min, and measure on the machine. The measurement results are shown in Table 6.

[0082]

[0083] Note: <LOQ means below the detection limit

[0084] It can be seen that benzene and o-xylene were detected in Si's blood, and the concentration of benzene in the sample was relatively high, while the concentration of o-xylene was low. It is suggested that the residents should pay attention to prevent long-term direct or indirect contact with benzene series, and should take a series of prevention and control measures to protect their physical and mental health.

Embodiment 3

[0086] Take 5 mL of blood from Yao, a resident of a key polluted area, and put it into a 40 mL sample bottle according to the above method, followed by adding 200 μL of isotope internal standard solution, a drop of antifoaming agent and 20 mL of pure water. Seal the vial and shake it up and down 20 times. Then fill the sample bottle with pure water and seal the sample bottle. Shake in an ultrasonic oscillator for 1 min, and measure on the machine. The measurement results are shown in Table 7.

[0087]

[0088] Note: <LOQ means below the detection limit

[0089] It can be seen that benzene and toluene were detected in Yao's blood, and the concentration of benzene in the sample was relatively high, while the concentration of toluene was relatively low. It is suggested that the residents should pay attention to prevent long-term direct or indirect contact with benzene series, and should take a series of prevention and control measures to protect their physical and mental he...

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Abstract

The invention relates to a method for detecting benzene series in a biological sample blood. The method for detecting benzene series in a biological sample blood is a method for simultaneously detecting the content of six benzene series in the blood by a purging and trapping / gas chromatography / mass spectrometry technology. Purging and trapping experiment conditions are optimized to enrich and desorb the six benzene series in the blood, and qualitative and quantitative analysis is carried out by gas chromatography / mass spectrometry and isotope internal standard method selected characteristic ions. The method has the advantages of simplicity in operation, short analysis period, good separation degree, and high accuracy and precision meeting the requirements of analysis and testing, and is suitable for the simultaneous detection and analysis of the benzene series in the biological sample blood.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for measuring benzene series in biological sample blood. Background technique [0002] Benzene series are important raw materials and solvents in chemical, pharmaceutical, pesticide, leather and other industries. Because benzene series have strong volatility and widely exist in the environment, they have become an important occupational hazard factor because of their wide exposure routes. Because benzene series are extremely harmful to human health, such as causing damage to the hematopoietic system and causing teratogenic and carcinogenic effects. Therefore, how to effectively measure the internal exposure content of benzene series is a hot spot in the field of environmental medicine and an urgent problem to be solved. [0003] At present, there are many detection methods for benzene series in air and water, and each method has been well known ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045
Inventor 秦小江侯晓敏聂继盛张坤张红梅
Owner CHENGDU TME SOFTWARE
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