A kind of method for preparing Qinling rock cabbage by biological tissue culture

Abstract

The invention provides a method for planting bergenia scopulosa through biological tissue culture. The method comprises the following steps: preparing a sterile female parent; trimming an explant, and inoculating the explant into a primary induction culture medium; transferring a test tube seedling after primary induction culture into a multiplying culture medium, and performing culture, wherein the multiplying culture medium comprises 0.5-2 mg / L MS+ZT, 0.05-1 mg / L KT, 0.02-0.2 mg / L IBA and 2-10 mg / L riboflavin; performing rooting culture on test tube seedlings grown from proliferation and differentiation in a culture medium of 0.5-2.0 mg / L MS+IBA and 2-10 mg / L riboflavin; when root systems of the test tube seedlings grow to 4-6 cm, transplanting into a mixed medium, and performing heat preservation and ventilation, wherein the mixed medium comprises perlite, turfy soil and peat; performing domestication in a greenhouse, and performing transplanting and outdoor culture. By adopting the method, the multiplication velocity of the bergenia scopulosa is increased, and standard production of the bergenia scopulosa can be achieved.

Description

Technical field

[0001] The invention belongs to the field of biology, relates to Qinling Bergenia, and specifically is a method for preparing Qinling Bergenia by biological tissue culture. Background technique

[0002] Qinling Bergenia (Bergenia scopulosa TPWang) is a perennial herb of Saxifrage family Bergenia. There are 10 species of this genus in the world, and 7 species are produced in China. Qinling Bergenia (its rhizome is in Meixian County and Qinling Mountains, Shaanxi) Known as Panlongqi) is not only one of the three endemic species in my country, but also the most primitive species of this genus. Domestic wild species are mostly distributed in Shaanxi, Sichuan and southeastern Gansu. In the winter, there are more withered and fallen leaves in the place of origin, but they bloom earlier, the flowers are pink, and have high ornamental value ( Figure 1 ~ Figure 2 ). The whole plant contains bergenin, and the rhizome contains relatively high drug content. It has been includ...

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