A kind of method for preparing Qinling rock cabbage by biological tissue culture

A technology of biological tissue and rock cabbage, applied in the field of biology, can solve the problems of low reproductive rate of wild Qinling rock cabbage, achieve the effect of improving survival rate and obvious technical effect

Active Publication Date: 2019-03-29
SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of method that biological tissue culture prepares rock cabbage Qinling, the cultivation method of described this rock cabbage Qinling will solve the technical problem of low reproduction rate of wild rock cabbage Qinling in the prior art

Method used

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  • A kind of method for preparing Qinling rock cabbage by biological tissue culture
  • A kind of method for preparing Qinling rock cabbage by biological tissue culture
  • A kind of method for preparing Qinling rock cabbage by biological tissue culture

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Experimental program
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Effect test

Embodiment 1

[0027] 1Materials and methods

[0028] 1.1 Test materials

[0029] Qinling rock cabbage is taken from the Qinling Mountains at an altitude of 1800m. After half a year of cultivation in Shanghai, select healthy, disease-free and pest-free plants as the sampling female parent, take the female parent's apical buds and the seedling apical buds from the ramets as the starting explants, remove the roots and leaves, and trim the stem tips for disinfection After the initial culture.

[0030] 1.2 Culture conditions

[0031] The sterile seedlings are cultivated under light conditions, with an illumination of 1500~2500lx, a photoperiod of 10~14h / d, and a temperature of 25℃. The pH of each medium was adjusted to 5.75, sucrose 3%, agar 0.64%, and kept in an autoclave at 121°C for 18 minutes after sub-packaging.

[0032] 1.3 Obtaining aseptic vaccine

[0033] The cutting and treatment of Qinling Bergenia explants are as follows: After rinsing with tap water, remove the leaves and thick fleshy rhizo...

Embodiment 2

[0047] Example 2 Differentiation and Proliferation Culture

[0048] 1. Medium formula design

[0049] In the proliferation stage, MS is used as the basic medium, and ZT is set to 5 concentrations of 0.1, 0.5, 1.0, 2.0, and 3.0 mg / L; KT is set to 6 concentrations of 0.01, 0.05, 0.1, 0.5, 1, 2 mg / L ; IBA is set to 6 concentrations of 0.01, 0.02, 0.05, 0.1, 0.2, 0.3mg / L; at the same time, riboflavin 2~10mg / L is added.

[0050] 2. Differentiation and proliferation

[0051] The test-tube plantlets induced on the primary induction medium, when they gradually grow up and a certain amount of test-tube plantlets are obtained, select the test-tube plantlets with a height of 0.5 cm and a bright green color, and transfer them to the proliferation medium in the same batch to select the appropriate proliferation medium. . The results showed that when the ZT concentration was 0.5-2 mg / L, the proliferation coefficient reached 2 or more, and when the concentration was 1 mg / L, the proliferation coeff...

Embodiment 3

[0060] Example 3 Rooting culture

[0061] At the rooting stage, MS was used as the basic medium, and IBA was set to 6 concentrations of 0.1, 0.25, 0.5, 1.0, 2.0, and 3.0 mg / L, and riboflavin was added at 2-10 mg / L. When the concentration of the additional auxin IBA is lower than 2.0mg / L, the rooting rate increases with the increase of the concentration, and the time to produce adventitious roots decreases with the increase of the concentration. When it exceeds this threshold, the rooting rate begins to decrease, and a small amount is produced at the same time Callus. In the concentration range of 0.5~2.0mg / L, the root primordium is partially formed in the seedling base after 15 days of culture, and it can grow to 2~4cm after 30 days. The rooting rate reaches 65% or more. The leaf color is dark green and stretched, forming a complete test tube seedling. . Screening 0.5~2.0mg / L as the appropriate concentration for rooting of Qinling Rock Cabbage. When IBA is 1.0mg / L, the rooting ...

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Abstract

The invention provides a method for planting bergenia scopulosa through biological tissue culture. The method comprises the following steps: preparing a sterile female parent; trimming an explant, and inoculating the explant into a primary induction culture medium; transferring a test tube seedling after primary induction culture into a multiplying culture medium, and performing culture, wherein the multiplying culture medium comprises 0.5-2 mg / L MS+ZT, 0.05-1 mg / L KT, 0.02-0.2 mg / L IBA and 2-10 mg / L riboflavin; performing rooting culture on test tube seedlings grown from proliferation and differentiation in a culture medium of 0.5-2.0 mg / L MS+IBA and 2-10 mg / L riboflavin; when root systems of the test tube seedlings grow to 4-6 cm, transplanting into a mixed medium, and performing heat preservation and ventilation, wherein the mixed medium comprises perlite, turfy soil and peat; performing domestication in a greenhouse, and performing transplanting and outdoor culture. By adopting the method, the multiplication velocity of the bergenia scopulosa is increased, and standard production of the bergenia scopulosa can be achieved.

Description

Technical field [0001] The invention belongs to the field of biology, relates to Qinling Bergenia, and specifically is a method for preparing Qinling Bergenia by biological tissue culture. Background technique [0002] Qinling Bergenia (Bergenia scopulosa TPWang) is a perennial herb of Saxifrage family Bergenia. There are 10 species of this genus in the world, and 7 species are produced in China. Qinling Bergenia (its rhizome is in Meixian County and Qinling Mountains, Shaanxi) Known as Panlongqi) is not only one of the three endemic species in my country, but also the most primitive species of this genus. Domestic wild species are mostly distributed in Shaanxi, Sichuan and southeastern Gansu. In the winter, there are more withered and fallen leaves in the place of origin, but they bloom earlier, the flowers are pink, and have high ornamental value ( Figure 1 ~ Figure 2 ). The whole plant contains bergenin, and the rhizome contains relatively high drug content. It has been includ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 吕秀立冯永辉张春英姚春霞沈烈英施季森
Owner SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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