A protein and application for inducing experimental autoimmune encephalomyelitis animal model in cynomolgus monkeys

An autoimmune and encephalomyelitis technology, which is applied to the protein and application field of the animal model of experimental autoimmune encephalomyelitis induced in cynomolgus monkeys, can solve the problems of adverse effects and poor results, and achieve docile and economical The effect of the test product and short breeding cycle

Active Publication Date: 2021-06-11
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most drugs, especially biotechnology drugs, have remarkable effects when used in animal models such as rodents, rabbits, and pigs, but the clinical evaluation has poor effects or even adverse effects. and pathophysiological differences

Method used

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  • A protein and application for inducing experimental autoimmune encephalomyelitis animal model in cynomolgus monkeys
  • A protein and application for inducing experimental autoimmune encephalomyelitis animal model in cynomolgus monkeys
  • A protein and application for inducing experimental autoimmune encephalomyelitis animal model in cynomolgus monkeys

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Experimental program
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Effect test

Embodiment 1

[0030] 1. Construction of recombinant plasmid pET28a-rhMOG 30-154 and corresponding recombinant engineered bacteria

[0031] The amino acid sequence of hMOG was checked by NCBI (GenBank: AAH35938.1), and its 30th-154th amino acid sequence (polypeptide) was named rhMOG 30-154 , the specific amino acid sequence is shown in SEQ ID NO.1, rhMOG was synthesized by Shanghai Jierui Bioengineering Co., Ltd. 30-154 The coding gene of the pET-28a(+) plasmid itself is used to select the appropriate restriction site NcoI and XhoI, and insert the same NcoI and XhoI double digestion site after NcoI and XhoI double digestion In the pET28a vector plasmid, construct the recombinant plasmid pET28a-rhMOG 30-154 , the specific construction method is as figure 1 shown.

[0032] The recombinant plasmid pET28a-rhMOG 30-154 Transformed into Escherichia coli DH5a competent cells, spread on LB solid medium (containing 50 μg / mL Kan), cultured at 37°C for 12-24 hours, picked a single colony from the ...

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Abstract

The invention discloses a protein for inducing an animal model of cynomolgus monkey experimental autoimmune encephalomyelitis and its application. The rhMOG 30‑154 Protein, the amino acid sequence of which is shown in SEQ ID NO.1. The invention innovatively uses human MOG extracellular antigen domain to recombine human MOG 30‑154 (hereinafter referred to as rhMOG 30‑154 ) subcutaneous multi-point immunization method to establish a relapsing-remitting cynomolgus monkey EAE animal model (cynomolgus monkey experimental autoimmune encephalomyelitis model) that is more similar to human pathological characteristics.

Description

Technical field: [0001] The invention belongs to the field of medical research, in particular to a protein for inducing an animal model of cynomolgus monkey experimental autoimmune encephalomyelitis and its application. Background technique: [0002] Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS), manifested by focal inflammatory infiltration (optic nerve, brain, spinal cord), demyelination, and axonal damage , Gliosis, etc., are difficult and serious diseases of the clinical nervous system. Appropriate animal models are needed for the study of its pathological process and pathogenesis, as well as the screening and evaluation of therapeutic drugs. Experimental autoimmune encephalomyelitis (EAE) is a commonly used animal model of multiple sclerosis. This model can demyelinate white matter and dark gray matter, and its biochemical, immune and pathological characteristics are very similar to those of MS. Therefore, it is widely used...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/705A01K67/027
CPCA01K67/0271A01K2207/10A01K2227/106A01K2267/0325C07K14/705
Inventor 孙云霄彭真张礼标吴洁何向阳彭兴文
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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