Peptide nucleic acid of UGT1A1 gene polymorphism detection primer and kit thereof

Abstract

The invention discloses peptide nucleic acid of a UGT1A1 gene polymorphism detection primer, and the nucleic acid comprises a forward primer UGT1A1-F1, a reverse primer UGT1A1-R1, a forward primer UGT1A1-F2, a reverse primer UGT1A1-R2 and peptide nucleic acid, wherein the nucleotide sequence of the forward primer UGT1A1-F1 is as shown in the SEQ ID NO.1; the nucleotide sequence of the reverse primer UGT1A1-R1 is as shown in the SEQ ID NO.2; the nucleotide sequence of the forward primer UGT1A1-F2 is as shown in the SEQ ID NO.3; the nucleotide sequence of the reverse primer UGT1A1-R2 is as shown in the SEQ ID NO.4; the nucleotide sequence of the peptide nucleic acid is as shown in the SEQ ID NO.5; and the 5' ends of the reverse primer UGT1A1-R1 and the reverse primer UGT1A1-R2 are provided with fluorescence marks. The UGT1A1 gene polymorphism detection kit disclosed by the invention has high sensitivity, low template amount, high speed, accuracy and low cost.

Description

technical field [0001] The invention relates to a gene polymorphism detection product and a detection system used in the product, belonging to the field of biotechnology. Background technique [0002] Irinotecan (CPT-11) belongs to a new family of cytotoxic drugs - camptothecin drugs, is a semi-synthetic soluble camptothecin derivative, is currently widely used in clinical topoisomerase I inhibitors agent. It is a broad-spectrum antineoplastic drug and has been widely used in the treatment of colorectal cancer, lung cancer, gastric cancer, cervical cancer, ovarian cancer and other malignant tumors. After entering the body, CPT-11 is converted into the active form 7-Ethyl-10-Hydroxycamptothecin (7-Ethyl-10-Hydroxycamptothecin, SN-38) under the action of carboxy esterases (CE); SN -38 is a topoisomerase I inhibitor. By inhibiting topoisomerase I, it prevents the repair of DNA single-strand breaks, thereby interfering with DNA replication, affecting transcription levels, lead...

AI Technical Summary

Problems solved by technology

[0005] At present, the detection of UGT1A1 gene polymorphism is mainly by sequencing method, which is cumbersome, costly, and requires a large amount of templates

However, a few methods using ABI capillary electrophoresis use the first-generation fluorescent dyes in the blue-green light region, and natural autofluorescence is also in the blue-green light region, so they are susceptible to natural autofluorescence interference and affect the accuracy of result interpretation.

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