SPR (surface plasma resonance) analyzing method for determining rutin and protein binding level
A technology of surface plasmon and analysis method, which is applied in the field of surface plasmon resonance analysis to determine the binding level of rutin and protein, and can solve the problems of rutin solubility reduction and other problems
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[0044] Embodiment: The analysis method of the present invention is used to determine the equilibrium constant of the interaction between rutin and human serum albumin (HSA). Specific steps are as follows.
[0045] 1. Use a new sensor chip, which is a glass plate coated with a bare gold film. Install it into the SPR instrument, start the instrument, and maintain the temperature of the chip area at 37°C.
[0046] 2. Use 5mmol / L 3-mercaptopropionic acid aqueous solution to circulate for 30 minutes at a flow rate of 5 microliters / minute, and control the temperature of the chip area at 37°C. After completion, rinse the pipeline and chip surface with water.
[0047] 3. Dissolve HSA in acetic acid-sodium acetate buffer solutions with a sodium ion concentration of 10 mmol / L and a pH of 4.0, 4.5, 5.0, and 5.5, respectively, and circulate for 5 minutes at a flow rate of 5 μl / min. The pH of the solution with the largest signal change at the end of the circulation is 5.5, so the buffer ...
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