Human urine detection reagent for monohydroxyphenyl metabolites

A technology for monohydric phenols and detection reagents, which is applied in the field of human urine detection reagents for monohydric phenol metabolites, and can solve problems such as misdiagnosis, short storage time of reagents, and limited application value

Active Publication Date: 2017-08-18
浙江道家医疗科技有限公司
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the complex composition of urine, certain trace components or abnormal metabolic products caused by other causes will also combine with mercury ions and mercury ions in the detection reagent to form color precipitates, which will seriously interfere with the accuracy of the test results and cause misdiagnosis. limit its application value
[0005] In addition, mercury ions and mercury ions in the detection reagent are affected by the environment and are easily hydrolyzed, so the storage time of the reagent is short and the stability is poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Human urine detection reagent for monohydroxyphenyl metabolites
  • Human urine detection reagent for monohydroxyphenyl metabolites
  • Human urine detection reagent for monohydroxyphenyl metabolites

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] In one embodiment, the preparation method of the described mercury ion mesoporous silica comprises the following steps:

[0035](1) Preparation of mesoporous silica: Add ethylene oxide-propylene oxide-ethylene oxide triblock copolymer and nitric acid into the reactor, and stir until the solid is completely dissolved at a temperature of 40°C. Then ethyl orthosilicate and sodium dodecylsulfonate were added dropwise to the solution, the reaction was continued for 2 hours, and then the temperature was adjusted to 70° C. for 72 hours. The obtained gel was soaked in alcohol, stirred and washed 3 times, filtered and dried in an oven at 100°C, and finally the obtained sample was calcined in a muffle furnace at 550°C for 6 hours to remove the template agent to obtain the mesoporous silica;

[0036] (2) Preparation of modified mesoporous silica: add mesoporous silica, toluene, 1,3-bis(3-aminopropyl)-1,1,3,3-tetramethyl to the reactor Disiloxane, mixed into a suspension, refluxe...

Embodiment 1

[0052] The raw materials for the preparation of the monohydric phenol metabolite human urine detection reagent include mercuric sulfate solution, mercurous nitrate solution, nickel sulfate solution, silicotungstic acid solution, trichloroacetic acid solution, mercurous ion mesoporous silica, acetic acid / Sodium acetate buffer solution; the concentration of the mercuric sulfate solution is 2.1mol / L; the concentration of the mercurous nitrate solution is 0.3mol / L; the concentration of the nickel sulfate solution is 0.02mol / L; the silicotungstic acid The concentration of solution is 3.2mmol / L; The mass fraction of described mercury ion mesoporous silica is 4.5%; The concentration of described acetic acid / sodium acetate buffer solution is 3mol / L; Described silicotungstic acid solution and described The concentration ratio of trichloroacetic acid solution is 1:0.64;

[0053] A preparation method of a monohydric phenol metabolite human urine detection reagent, comprising the followi...

Embodiment 2

[0060] The raw materials for the preparation of the monohydric phenol metabolite human urine detection reagent include mercuric sulfate solution, mercurous nitrate solution, nickel sulfate solution, silicotungstic acid solution, trichloroacetic acid solution, mercurous ion mesoporous silica, acetic acid / Sodium acetate buffer solution; the concentration of the mercuric sulfate solution is 2.1mol / L; the concentration of the mercurous nitrate solution is 0.3mol / L; the concentration of the nickel sulfate solution is 0.02mol / L; the silicotungstic acid The concentration of solution is 3.2mmol / L; The mass fraction of described mercury ion mesoporous silica is 4.5%; The concentration of described acetic acid / sodium acetate buffer solution is 3mol / L; Described silicotungstic acid solution and described The concentration ratio of trichloroacetic acid solution is 1:0.5;

[0061] A preparation method of a monohydric phenol metabolite human urine detection reagent, comprising the followin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a human urine detection reagent for monohydroxyphenyl metabolites. The human urine detection reagent for the monohydroxyphenyl metabolites comprises mercury sulfate solution, mercury nitrate solution, nickel sulfate solution, silicotungstic acid solution, trichloroacetic acid solution and mercury ion (i) mesoporous silica. The concentration of the mercury sulfate solution is 0.7-3 mol / L; the concentration of the mercury nitrate solution is 0.1-0.6 mol / L; the concentration of the nickel sulfate solution is 0.01-0.05 mol / L; the concentration of the silicotungstic acid solution is 1.0-4.0 mmol / L; the concentration of the trichloroacetic acid solution is 0.6-5.0 mmol / L; the mass fraction of the mercury ion (i) mesoporous silica is 3-10%.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular, the invention relates to a human urine detection reagent for monohydric phenol metabolites. Background technique [0002] Cancer mortality ranks first among all diseases and is the number one killer of human health. It has become a major cause of threat to human life and health. Therefore, the prevention and treatment of cancer has become one of the priorities of global health strategies, and the key to treating cancer is Early detection, early diagnosis and early treatment. Various imaging detection techniques, such as X-ray, B-ultrasound, CT, and magnetic resonance, as important auxiliary diagnostic methods for tumors, usually cannot find small early tumors, and require special and expensive equipment investment, so there are limitations in application. Certain limitations, especially not suitable for large-scale tumor screening and use in grassroots units or remote a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50C01B33/12
CPCC01B33/12G01N33/50
Inventor 胡振皖
Owner 浙江道家医疗科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap